Search Results (88)

Triticale (Triticosecale Wittmack) is a versatile forage crop valued for its high yield, balanced nutrition, and environmental adaptability. However, the dough-stage triricale has higher dry matter and starch content but lower water-soluble carbohydrate levels than earlier stages, posing fermentation challenges that may impair silage quality. This study aimed to investigate the effects of lactic acid bacteria inoculation on the fermentation quality, bacterial community, and metabolome of whole-plant triticale silage at the dough stage. Fresh triticale was ensiled for 30 days without or with an inoculant containing Lactiplantibacillus plantarum and Streptococcus bovis. Fermentation quality, bacterial succession, and metabolic profiles were analyzed at multiple time points. Inoculation significantly improved fermentation quality, characterized by a rapid pH drop, increased lactic acid production, and better preservation of fiber components. Microbial analysis revealed that inoculation successfully established Lactobacillus as the dominant genus while suppressing spoilage bacteria like Enterobacter and Clostridium. Metabolomic analysis on day 30 identified numerous differential metabolites, indicating that inoculation primarily altered pathways related to amino acid and purine metabolism. In conclusion, inoculating dough-stage triticale with this LAB combination effectively directs the fermentation trajectory. It enhances silage quality not only by optimizing organic acid profiles and microbial succession but also by modulating key metabolic pathways, ultimately leading to improved nutrient preservation. Full article

After legumes, cereals are the most important source of protein for humans and livestock worldwide. One way to meet growing nutritional demands is to increase the grain protein content (GPC) of cereals. Breeding advances in this regard should be supported by optimized agricultural practices. The GPCs of winter rye, winter triticale, spring barley, and spring oats grown in 2018–2022 in northeast Poland were evaluated to determine the influence of the crop sequence system (continuous monocropping, crop rotation), cultivar (two for each species), plant protection level (control treatment, herbicide, herbicide, and fungicide), and interactions among these factors. The cultivar selection was a significant GPC determinant in all cereals. Growing triticale in crop rotation after a legume increased its GPC compared to continuous monocropping, but decreased the GPC of rye and had no effect on the GPCs of spring cereal that followed non-legume crops. Using herbicides and herbicides combined with fungicides promoted the GPC of rye and oats, but not of triticale and barley. The heterogeneity of the interaction effects of the studied agricultural practices on the GPCs of the individual cereals prevents the identification of a universal combination that would ensure the highest GPC levels. The inter-annual weather variability played a significant role in shaping the GPCs of cereals and in modifying the influence of the controlled factors. Full article

Triticale (× Triticosecale Wittmack) is a valuable dual-purpose crop due to its adaptability to marginal environments and its potential for both high-quality grain and forage production. However, a comprehensive evaluation of its forage quality characteristics and agronomic performances is still needed. This study evaluated the grain and forage yield potentials and nutritional compositions of 11 triticale genotypes over two consecutive years in a semi-arid region located in Shanxi province, China. Forage quality was assessed using several key parameters, including nutrient composition, fiber digestibility, mineral content, and energy density, while grain quality parameters, including nutrient composition as well as carbohydrate and fiber characteristics, were also analyzed. Significant genetic variation was observed in these traits, indicating the influence of genotype–environment interactions on these traits. The tested genotypes exhibited grain yields ranging from 4.83 to 6.92 t ha⁻¹ and fresh forage biomass yields between 20.06 and 29.78 t ha⁻¹, demonstrating their potential for sustainable forage and grain production under semi-arid conditions. Genotypes from our breeding programs, including Shengnongsicao 1 and Jinsicao 1, demonstrated superior adaptability, maintaining stable forage and grain yield potentials under adverse conditions. Their favorable nutritional characteristics further enhance their suitability for semi-arid livestock systems. High levels of essential minerals, particularly calcium and potassium, further enhanced the nutritional value of these genotypes. These results provide valuable insights for triticale breeding programs and suggest triticale’s potential as a reliable crop in semi-arid regions, where maximizing land productivity is essential. Full article

Small-grain producers in the southern Pannonian Plain prefer winter barley production in poor soils and drought-prone areas, assuming higher resource use efficiency in barley than in wheat. Similarly, triticale is known to perform well in low-fertility soils and dry environments. However, information about the comparative performance of these crops within the same trials is less available for the Pannonian environment. Therefore, this study aimed to compare the grain yield and nitrogen use efficiency traits of winter wheat, triticale, and two-rowed and six-rowed barley cultivars across different N applications in different growing seasons and locations in the Pannonian Plain. The study was conducted over two seasons at three locations (Novi Sad, Sremska Mitrovica, and Sombor) using a split-plot design. Treatments consisted of winter wheat, triticale, and two-rowed and six-rowed barley under three nitrogen fertilization levels of low, moderate, and high. Averaged across species, the reduction in grain yield in 0 N compared to 100 N was 1218 kg ha⁻¹ (15.7%) in wheat, 1037 kg ha⁻¹ (11.6%) in triticale, 1128 kg ha⁻¹ (13.7) in two-rowed barley, and 1340 kg ha⁻¹ (17.1%) in six-rowed barley. Grain yield was closely related to nitrogen uptake, showing a relationship (R²) from 0.652 in triticale to 0.956 in six-rowed barley. Nitrogen use efficiency showed a positive relationship with nitrogen uptake efficiency, while the relationship with nitrogen utilization efficiency was insignificant. There was a notable difference between crops in terms of grain yield and nitrogen use efficiency traits. Notably, two-rowed barley outperformed wheat in terms of grain yield and nitrogen use efficiency, while wheat outperformed six-rowed barley. Triticale showed the highest yield among all the studied cereal crops, attributed to increased nitrogen use efficiency and uptake, especially under low fertilization conditions. Full article

Due to the increasing demand for food, the agricultural sector is facing a huge challenge related to its production without having a negative impact on the environment. The above issues are regulated by the current EU policy, indicating the need to conduct plant production by the European Green Deal strategy (2020), based on reducing the use of chemical plant-protection products by 50% and reducing the level of mineral fertilization by 20% by 2030. Using appropriate techniques of applying mineral fertilizers, which have a positive effect on the development and activity of soil microorganisms, the availability of nutrients in the soil can be increased while reducing fertilizer applications. This study aims to show whether the use of foliar nitrogen fertilizers in combination with sulfur in a two-year field experiment with triticale stimulates the multiplication of selected groups of microorganisms and the level of soil enzymatic activity. In addition, effects on the content of available forms of nutrients in the soil, triticale yield, and total nutrient uptake were analyzed. It is shown that the applied foliar fertilizers enriched with sulfur, compared to fertilization with ammonium nitrate, contributed to a 100% increase in the number of analyzed groups of microorganisms, urease, and alkaline phosphatase activity; a 44% increase in dehydrogenase activity; and a 15% increase in acid phosphatase activity. The hybrid application technique, with reduced doses of nitrogen fertilizers enriched with sulfur by 20 and 25%, resulted in grain yields exceeding 8 t/ha. Moreover, compared to conventional fertilization based on ammonium nitrate, an increase in the grain yield of triticale was obtained in the range of 16 to 24%, as well as an increase in the accumulation of nutrients in the plant. It is shown that foliar fertilization is a promising alternative to standard fertilization techniques. Full article

The aim of this study was to investigate the effects of different N application rates on the yield, N accumulation, and grain quality of triticale and provide a theoretical basis for selecting the best N application rate for sowing triticale in the loess parent material area. This experiment was conducted at the experimental station of Shanxi Agricultural University in Jinzhong City, Shanxi Province, China, from 2021 to 2023 to study the effects and differences of different N application rates (N application rates 180, 210, 240, 270, and 300 kg ha⁻¹) on the yield formation, N utilization, and quality of the two triticale varieties Donghei10 (DH10) and Taizi6336 (TZ6336). The results showed that, compared with other N application rates, N240 increased the number of spikes and grains per spike of triticale and increased the yield by 6–19%. The yield increased most significantly when the N application range was 210–240 kg ha⁻¹; the yield change rate per 2 kg of N increase was the largest within this range, and the continued increase in yield with increasing N application was slow. N240 also improved the NUE, NUPE, nutritional quality, and processing quality of triticale. The results of the pathway analysis showed that the N application rate affected the spike number, grain number per spike, and 1000-grain weight of triticale. N accumulation at the anthesis and maturity stages affected GS and GOGAT activities in the flag leaf and grain, thus affecting the N efficiency, yield, and protein content. Overall, a N application at 240 kg ha⁻¹ increased the yield, nitrogen efficiency, and quality of triticale, and there was no significant difference in yield between DH10 and TZ6336. Full article

The aim of this study was to investigate the effects of extrusion processing parameters—moisture content (M = 20 and 24%), feeding rate (FR = 20 and 25 kg/h), and screw speed (SS = 300, 390 and 480 RPM), on the content of deoxynivalenol (DON), 15-Acetyl Deoxynivalenol (15-AcDON), 3-Acetyl Deoxynivalenol (3-AcDON), HT-2 Toxin (HT-2), tentoxin (TEN) and alternariol monomethyl ether (AME), using a pilot single-screw extruder in whole-grain triticale flour. The temperature at the end plate of the extruder ranged between 97.6 and 141 °C, the absolute pressure was from 0.10 to 0.42 MPa, the mean retention time of material in the barrel was between 16 and 35 s, and the specific energy consumption was from 91.5 to 186.6 Wh/kg. According to the standard score, the optimum parameters for the reduction of the content of analysed mycotoxins were M = 24 g/100 g, FR = 25 kg/h, SS = 480 RPM, with a reduction of 3.80, 60.7, 61.5, 86.5, 47.7, and 55.9% for DON, 3-AcDON, 15-AcDON, HT-2, TEN, and AME, respectively. Under these conditions, the bulk density, pellet hardness, water absorption index, and water solubility index of the pellet were 0.352 g/mL, 13.7 kg, 8.96 g/g, and 14.9 g/100 g, respectively. Full article

Due to the high yield potential, suitable agrotechnical properties, and nutritional value of the grain, the interest in growing triticale is increasing due to the high yield potential, suitable agrotechnical properties, and nutritional value. This species is primarily grown for fodder purposes, but numerous studies suggest its potential for human consumption, including bread production. Additionally, triticale is known for its greater resistance to adverse environmental conditions compared to other crops, even under varying agronomic practices. A field experiment was conducted in southeastern Poland from 2019 to 2022. The study involved two cultivation systems (conventional and integrated) as one factor and three winter triticale cultivars (Belcanto, Meloman, and Panteon) as the other. The conventional system is based on the intensive cultivation of plants through the use of large amounts of fertilizers and crop protection products. The integrated system of cultivation is an alternative to the conventional system. This system aims to reduce the use of industrial inputs and, as a result, minimize the negative impact of agriculture on the natural environment. Cultivation under the conventional system resulted in higher grain yields and improved physiological parameter values. There was an increase in the leaf area index (LAI), relative chlorophyll content, chlorophyll fluorescence parameters, and gas exchange parameters (photosynthetic rate (Pn) and transpiration rate (E)). The highest yields were achieved with the cv-Panteon and cv-Belcanto under the conventional system. The yields of these cultivars in the integrated system did not differ significantly from those of cv-Meloman under the conventional system. In the 2021/2022 season, the weather conditions were the most favorable during the triticale vegetation period, which resulted in the highest grain yield. The conventional system also resulted in higher thousand-grain weight (TGW), crude protein content, and grain test weight while lowering the falling number (FN) value. However, the cultivation systems did not significantly affect the grain uniformity, crude fat, fiber, or ash content, as well as wet gluten and gluten index (GI). The cv-Panteon exhibited the highest level of crude protein, crude fiber, and crude ash in its grain, suggesting its strong nutritional value and potential for use in human consumption. The cultivation of triticale in the integrated system, although associated with lower yields, causes less environmental pollution than cultivation in the conventional system. The appropriate selection of efficient cultivars grown in the integrated system allows for high grain yields with good quality parameters. Full article

(1) Background: Suitable harvest maturity stage selection and microbial inoculation during anaerobic fermentation are effective strategies for improving the quality of triticale (×Triticosecale) silage for ruminant nutrition. (2) Methods: In the present study, the fermentation characteristics, microbial communities, and their correlations were evaluated for triticale silages, as affected by Lactiplantibacillus plantarum (LP) inoculation at the heading, flowering, filling, milk-ripening, and wax-ripening stages. (3) Results: The results indicate that the filling and milk-ripening stages without LP inoculation resulted in lower pH and higher lactic acid than other harvest maturity stages (p < 0.05). Inoculating with LP decreased the pH at each harvest maturity stage, except for the filling stage, and increased the lactic acid concentration at the heading and filling stages (p < 0.05). The bacterial dynamics indicated that the abundances of Lactiplantebacilli and Monascus of the triticale silages without the LP inoculation were different between the harvest maturity stages (p < 0.05), and the abundance of Enterobacters was different between the harvest maturity stages in the triticale silage (p < 0.05). Remarkably, negative correlations were found between the Lactiplantebacillus, Monascus, and pH and positive correlations were found between the Lactiplantebacillus, Monascus, and lactic acid content (p < 0.05). (4) Conclusions: The filling and milk-ripening stages were the most suitable harvest maturity stages for the triticale silage. Inoculation with LP could enhance the fermentation quality, increase the abundances of beneficial microorganisms, and inhibit harmful microorganisms in triticale silage. Full article

The use of native and malted triticale (MT) flour in dry pasta has been limited despite the potential of triticale in cereal-based food production. In this study, triticale-based dry spaghetti with increasing levels of substitution (0, 25, 50, and 75 g/100 g w/w) of MT flour were formulated and analyzed. Samples were analyzed for technological and nutritional traits, including the in vitro starch and protein digestions. The gradual substitution of native triticale flour with MT increased (p < 0.05) the total dietary fiber content, whereas total starch decreased (p < 0.05). Adding MT flour increased the cooking loss and the stickiness of cooked pasta (p < 0.05). Using MT flour modulated the in vitro starch digestion, lowering the slowly digestible and resistant starch contents. The in vitro protein digestibility was positively affected using MT at the highest substitution level. Overall, MT could be used to formulate dry pasta products being the substitution to native triticale up to 50 g/100 g, a good compromise between nutritional quality and technological characteristics. Full article

Zinc (Zn) deficiency represents a significant global concern, affecting both plant and human health, particularly in regions with Zn-depleted soils. Agronomic biofortification strategies, such as the application of Zn fertilizers, offer a cost-effective approach to increase Zn levels in crops. This study aimed to assess the efficacy of soil and foliar Zn biofortification, applied as an aqueous solution of 0.5% zinc sulphate (ZnSO₄·7H₂O), on triticale (x Triticosecale) grown under Mediterranean conditions. The study was conducted over two growing seasons (2017/18 and 2018/19) in southern Spain, evaluating the effects on biomass yield; forage quality, including crude protein, Van Soest detergent fiber, organic matter digestibility, and relative forage value; and nutrient accumulation. Soil treatment consisted in the application of 50 kg of ZnSO₄·7H₂O ha⁻¹ solely at the beginning of the first campaign to assess the residual effect on the second year. In contrast, the foliar treatment consisted of two applications of 4 kg of ZnSO₄·7H₂O ha⁻¹ per campaign, one at the beginning of tillering and the other at the appearance of the first node. The foliar application increased the Zn content of the forage to adequate levels, while the soil application resulted in a 33% increase in biomass production, which is particularly beneficial for farmers. Overall quality was favored by the combined soil + foliar application, and no adverse antagonistic effects on other nutrients were detected. Instead, a synergistic interaction between Se and Zn was observed, which improved the efficacy of this important micronutrient for livestock and human wellbeing. Full article

The ongoing scientific debate on the selection of the best bioindicators to reflect the quality of arable soils indicates both their microbiome and biochemical parameters. Consideration has also been given to the fact that Zea mays has achieved the status of a crop used in the feed industry and for energy purposes, and Triticosecale is attracting increasing interest in this area. Therefore, the aim of this study was to determine the wide range of effects of Zea mays and Triticosecale cultivation on soil microbial and biochemical activity. The assessment of these parameters was based on the determination of microbial abundance, colony development index (CD), ecophysiological index of microbial diversity (EP), soil enzyme activities (dehydrogenases, catalase, urease, acid phosphatase, alkaline phosphatase, β-glucosidase, and arylsulfatase) as well as soil physicochemical properties. The innovative nature of the research was achieved by extending the pool of analyses to include both microbial biodiversity and analysis of soil samples at three depths: 0–20 cm; 21–40 cm; and 41–60 cm. It was found that the highest activities of soil enzymes and the abundance of organotrophic bacteria and fungi, as well as their colony development indices (CD), occurred within the rhizosphere and that their values decreased with increasing depth of the soil profile layers. Two phyla, Actinobacteria and Proteobacteria, representing the microbiome of arable soils, were identified independently of soil management practices. Unique bacterial genera in the soil under Triticosecale cultivation were Pseudonocardia, whereas Rhodoplanes, Nocardioides, and Rhodanobacter were found under Zea mays cultivation. The activity of all enzymes, especially urease and arylsulfatase, was significantly higher in the soil under Triticosecale. This was influenced by the more favorable physicochemical properties of the soil. Full article

Climate change, which causes periods with relatively high temperatures in winter in Poland, can lead to a shortening or interruption of the cold hardening of crops. Previous research indicates that cold acclimation is of key importance in the process of acquiring cereal tolerance to stress factors. The objective of this work was to verify the hypothesis that both natural temperature fluctuations and the plant genotype influence the content of metabolites as well as proteins, including antioxidant enzymes and photosystem proteins. The research material involved four winter triticale genotypes, differing in their tolerance to stress under controlled conditions. The values of chlorophyll a fluorescence parameters and antioxidant activity were measured in their seedlings. Subsequently, the contribution of selected proteins was verified using specific antibodies. In parallel, the profiling of the contents of chlorophylls, carotenoids, phenolic compounds, and proteins was carried out by Raman spectroscopy. The obtained results indicate that a better PSII performance along with a higher photosystem II proteins content and thioredoxin reductase abundance were accompanied by a higher antioxidant activity in the field-grown triticale seedlings. The Raman studies showed that the cold hardening led to a variation in photosynthetic dyes and an increase in the phenolic to carotenoids ratio in all DH lines. Full article

The Tibetan Plateau is located in southwestern China. It has many important ecological functions, such as biodiversity protection, and is an important grassland agroecosystem in China. With the development of modern agriculture and animal husbandry, antibiotics are widely used to treat humans and livestock, and antibiotics cannot be fully metabolised by both. Antibiotics eventually find their way into the environment, affecting other parts of grassland agroecosystems. Triticale (Triticosecale wittmack) is an artificial hybrid forage that can be used for both grain and forage. This study revealed the diversity of seedborne bacteria in triticale on the Tibetan Plateau and the resistance of the bacteria to nine antibiotics. It identified 37 representative strains and successfully obtained the spliced sequences of 36 strains of the bacteria, which were clustered into 5 phyla and 16 genera. Among them, 18 strains showed resistance to at least one of the 9 antibiotics, and the colony-forming unit (CFU) abundance of antibiotic-resistant bacteria (ARB) accounted for 45.38% of the total samples. Finally, the bacterial motility and biofilm formation ability were measured, and their correlation with bacterial resistance was analysed. The results showed that the bacterial resistance did not have an absolute positive correlation with the motility or biofilm formation ability. Full article

Nitrogen (N) can enhance the biomass and feeding quality of forage crops and advance the growth of the herbivorous livestock industry. Investigating the N fertilizer dynamics in the soil–crop–livestock system is important for resource-use efficiency and environmental safety. By using the ¹⁵N-labeled technology and the in vitro incubation technique, an experiment was conducted in the North China Plain (NCP) in 2015–2016 to quantify the migration and distribution of N fertilizer in the soil–forage triticale (X Triticosecale Wittmack)–dairy cow system. The results showed that 34.1–37.3% of the applied N fertilizer was absorbed by forage triticale, in which 35.9–39.6% N accumulated in the stems and 60.4–64.1% accumulated in the leaves. In addition, 36.3–39.1% of the applied N fertilizer remained in the 0–100 cm soil layer, in which 81.8–91.3% was distributed in the 0–40 cm soil layer. The remaining 24.6–26.8% of the applied N fertilizer was lost in various ways and 28.1–31.3% of the N fertilizer could be utilized by dairy cows. When N fertilizer was applied between 0–225 kg N ha⁻¹, the increased application of N fertilizer improved the biomass yield from 14.0 to 17.5 t ha⁻¹ and enhanced the N content of the forage triticale from 1.3% to 1.4%; however, it did not significantly affect the distribution rate of N fertilizer in the soil–forage triticale–dairy cow system. The optimum N fertilizer application rate for forage triticale is less than 225 kg N ha^–1 to maintain high-efficient N use in the soil–crop–livestock system and reduce the environmental risks in the NCP. Our results quantified the N fertilizer dynamics in the soil–forage triticale–dairy cow system and provided a significant reference for guiding rational strategies of forage triticale cultivation. Full article