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Search Results (18)

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Authors = Md. Ashraful Hasan ORCID = 0000-0003-4008-5494

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24 pages, 824 KiB  
Article
MMF-Gait: A Multi-Model Fusion-Enhanced Gait Recognition Framework Integrating Convolutional and Attention Networks
by Kamrul Hasan, Khandokar Alisha Tuhin, Md Rasul Islam Bapary, Md Shafi Ud Doula, Md Ashraful Alam, Md Atiqur Rahman Ahad and Md. Zasim Uddin
Symmetry 2025, 17(7), 1155; https://doi.org/10.3390/sym17071155 - 19 Jul 2025
Viewed by 403
Abstract
Gait recognition is a reliable biometric approach that uniquely identifies individuals based on their natural walking patterns. It is widely used to recognize individuals who are challenging to camouflage and do not require a person’s cooperation. The general face-based person recognition system often [...] Read more.
Gait recognition is a reliable biometric approach that uniquely identifies individuals based on their natural walking patterns. It is widely used to recognize individuals who are challenging to camouflage and do not require a person’s cooperation. The general face-based person recognition system often fails to determine the offender’s identity when they conceal their face by wearing helmets and masks to evade identification. In such cases, gait-based recognition is ideal for identifying offenders, and most existing work leverages a deep learning (DL) model. However, a single model often fails to capture a comprehensive selection of refined patterns in input data when external factors are present, such as variation in viewing angle, clothing, and carrying conditions. In response to this, this paper introduces a fusion-based multi-model gait recognition framework that leverages the potential of convolutional neural networks (CNNs) and a vision transformer (ViT) in an ensemble manner to enhance gait recognition performance. Here, CNNs capture spatiotemporal features, and ViT features multiple attention layers that focus on a particular region of the gait image. The first step in this framework is to obtain the Gait Energy Image (GEI) by averaging a height-normalized gait silhouette sequence over a gait cycle, which can handle the left–right gait symmetry of the gait. After that, the GEI image is fed through multiple pre-trained models and fine-tuned precisely to extract the depth spatiotemporal feature. Later, three separate fusion strategies are conducted, and the first one is decision-level fusion (DLF), which takes each model’s decision and employs majority voting for the final decision. The second is feature-level fusion (FLF), which combines the features from individual models through pointwise addition before performing gait recognition. Finally, a hybrid fusion combines DLF and FLF for gait recognition. The performance of the multi-model fusion-based framework was evaluated on three publicly available gait databases: CASIA-B, OU-ISIR D, and the OU-ISIR Large Population dataset. The experimental results demonstrate that the fusion-enhanced framework achieves superior performance. Full article
(This article belongs to the Special Issue Symmetry and Its Applications in Image Processing)
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20 pages, 4532 KiB  
Article
Phenotypic and Biochemical Trait Improvement in Husk Tomatoes (Physalis sp.) through EMS-Induced Mutagenesis
by Md Ashraful Islam, Fabeeha Tarannum, Afsana Hossain Dina, Minhaz Ahmed, Md Ahsanul Haque, Sezai Ercişli, Md Golam Rasul, Duran Simsek and Mehfuz Hasan
Horticulturae 2024, 10(9), 913; https://doi.org/10.3390/horticulturae10090913 - 27 Aug 2024
Cited by 1 | Viewed by 1276
Abstract
Husk tomatoes are used in salsa and traditional medicine to alleviate illnesses. Market demand requires husk tomato varieties with improved agronomic and physicochemical health benefits. Mutagen application alters plant genomes, creating new traits and genetic diversity. The efficacy of EMS (ethyl methanesulfonate) was [...] Read more.
Husk tomatoes are used in salsa and traditional medicine to alleviate illnesses. Market demand requires husk tomato varieties with improved agronomic and physicochemical health benefits. Mutagen application alters plant genomes, creating new traits and genetic diversity. The efficacy of EMS (ethyl methanesulfonate) was examined for morphology, bioactive compounds, and phytochemical improvement. Three husk tomato genotypes representing Physalis philadelphica and Physalis ixocarpa underwent two hours of 1.4% EMS. In addition to yield and yield-related properties, total phenol, antioxidant activity, chlorophyll a and b levels in leaves and fruits, and phytochemical concentrations of Na, Mg, Ca, Mn, Fe, Cu, and K in fruits were measured. Genotype-dependent morphological changes were found. The mutants C1T6 (85 cm) and C1T7 (87.60 cm) were shorter than Control C1 (102 cm). The highest yield was 5.80 g for C1T5; Control C1 produced 3.08. The mutant C2T6 produced the most (5.99 g) compared to its control (2.85 g). Mutants had higher total phenol, antioxidant activity, and leaf/fruit chlorophyll. C1T2 had the highest antioxidant activity (1.19 ng/µL). C2T1 outperformed Control C2 at 1.54 ng/µL phenolic content. C3T2 had the highest Ca content (1822 µg/g), while Control C3 had 861.20 µg/g. Mutations altered phytochemical composition, which can be used to generate nutritionally superior husk tomato varieties. Additionally, scientists will be able to study mutants with advantageous morphological and biochemical traits, enabling extensive research. Furthermore, the mutants will serve as a genetic repository for the progression of breeding procedures. Full article
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20 pages, 3141 KiB  
Article
Enhanced Antioxidant Activity and Secondary Metabolite Production in Tartary Buckwheat under Polyethylene Glycol (PEG)-Induced Drought Stress during Germination
by Md. Shakhawat Hossain, Jing Li, Chenyang Wang, Fakhrul Islam Monshi, Rehenuma Tabassum, Md Ashraful Islam, Muhiuddin Faruquee, Md. Abdul Muktadir, Md Sultan Mia, A. K. M. Mominul Islam, Ahmed Khairul Hasan, Ashim Sikdar and Baili Feng
Agronomy 2024, 14(3), 619; https://doi.org/10.3390/agronomy14030619 - 19 Mar 2024
Cited by 8 | Viewed by 2831
Abstract
Drought stress is one of the key factors impeding agricultural productivity worldwide. This experiment aimed at investigating the polyethylene glycol (PEG)-induced drought stress effects on seed germination, physiology, and biochemical mechanisms in Tartary buckwheat genotypes. Four PEG-induced stress conditions (0%, 10%, 20%, and [...] Read more.
Drought stress is one of the key factors impeding agricultural productivity worldwide. This experiment aimed at investigating the polyethylene glycol (PEG)-induced drought stress effects on seed germination, physiology, and biochemical mechanisms in Tartary buckwheat genotypes. Four PEG-induced stress conditions (0%, 10%, 20%, and 30%) were applied to 14 selected genotypes at the germination stage to evaluate their stress tolerance capacity. Significant differences were obtained in germination percentage, relative water content (RWC), and all growth parameters among the studied 14 genotypes. Based on the stress tolerance index (STI), XiNong 9943, XiNong 9940, and QianKu-5 were found to be tolerant, and QuanKu-4 was susceptible. These cultivars were selected for further physiological and biochemical characterization. The results demonstrated that the activity of enzymes was significantly increased with the increase in PEG dose. SOD (superoxide dismutase), POD (peroxidase), CAT (catalase), and APX (ascorbate peroxidase) levels obtained at 30% PEG in the XiNong 9943 genotype were 2.01, 2.19, 4.92, and 4.46 times higher, respectively, than the normal growth condition (T0). Moreover, the secondary metabolite content also increased with the increase in PEG dose. At 30% PEG, the genotype XiNong 9943 yielded phenols, flavonoids, polyphenol oxidase (PPO), and phenylalanine ammonia lyase (PAL) levels that were higher by 131%, 95%, 154%, and 164%, respectively, than T0 condition. From both the findings of the activity of enzymes and the secondary metabolite content, the genotypic response to drought was ranked in the following order: XiNong 9943 > XiNong 9940 > QianKu-5 > QianKu-4, which supported the STI selection system. Assessing the overall performance, the genotype XiNong 9943 shows drought tolerance, which can be useful material for future buckwheat breeding programs. Full article
(This article belongs to the Section Plant-Crop Biology and Biochemistry)
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26 pages, 8702 KiB  
Article
Multifaceted Linkages among Eco-Climatic Factors, Plankton Abundance, and Gonadal Maturation of Hilsa Shad, Tenualosa ilisha, Populations in Bangladesh
by Mobin Hossain Shohan, Mohammad Abu Baker Siddique, Balaram Mahalder, Mohammad Mahfujul Haque, Chayon Goswami, Md. Borhan Uddin Ahmed, Mohammad Ashraful Alam, Md. Abul Bashar, Yahia Mahmud, Mahamudul Alam Chowdhury, Md. Mahmudul Hasan and A. K. Shakur Ahammad
Climate 2024, 12(3), 40; https://doi.org/10.3390/cli12030040 - 8 Mar 2024
Cited by 5 | Viewed by 3375
Abstract
An integrated multivariate approach was applied to gain a deeper understanding of the feeding biology of hilsa shad, Tenualosa ilisha, collected from six different aquatic habitats across Bangladesh. This approach involved linking climatic factors, ecological factors, plankton abundance in water, reproductive traits, [...] Read more.
An integrated multivariate approach was applied to gain a deeper understanding of the feeding biology of hilsa shad, Tenualosa ilisha, collected from six different aquatic habitats across Bangladesh. This approach involved linking climatic factors, ecological factors, plankton abundance in water, reproductive traits, and plankton ingestion data. Climatic data were obtained from the National Oceanic and Atmospheric Administration (NOAA) and Climate Data Online (CDO) databases on a monthly basis. Water quality parameters were observed on-site at various sampling sites. Plankton data from water bodies and hilsa guts were collected monthly from the study areas and analyzed in the laboratory. The results obtained were averaged for each month. The correlation tests, multivariate approaches, cluster analyses, and regression analyses revealed that the gonadosomatic index was primarily influenced by climatic factors, the abundance of ingested gut plankton, and heir compositions. The analysis of selectivity indices confirmed that plankton preferentially ingested selective taxa. Thirteen plankton groups were identified in the water column of six different hilsa habitats. The dominant phytoplankton groups were Bacillariophyceae (34–53%), Chlorophyceae (31–50%), Cyanophyceae (4–8%), and Euglenophyceae (1–3%). Additionally, Copepoda, Rotifera, and Cladocera were the most numerous zooplankton groups. Hilsa shad primarily consumed Bacillariophyceae (38–57%), Chlorophyceae (35–53%), and Cyanophyceae (4–6%). However, they also exhibited selective ingestion of higher quantities of Bacillariophyceae and Chlorophyceae to fulfill specific and unique metabolic needs. Cluster analysis revealed the variability of phytoplankton and zooplankton abundance in water and gut in relation to diverse locations. Combining all the datasets, a principal component analysis (PCA) was applied. The first two principal components explained more than 54% of the variability. The first component explained the association between the gonadosomatic index and eco-climatic factors, while the second component extracted the cluster of ingested gut plankton in relation to salinity and pH. Pearson’s correlations and linear regression analyses showed that the number of gut plankton had a positive influence on the gonadosomatic index (GSI). Finally, the outcomes from these extensive datasets have provided a better understanding of the selective feeding behavior and the influence of feeding biology on the gonadal maturation of T. ilisha. This understanding is likely to be useful for maintaining and improving the growth and productivity of the existing production systems for this transboundary species. Full article
(This article belongs to the Special Issue Climate Impact on Species Composition and Structure)
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10 pages, 579 KiB  
Review
Implementation and Delivery of Oral Cholera Vaccination Campaigns in Humanitarian Crisis Settings among Rohingya Myanmar nationals in Cox’s Bazar, Bangladesh
by Ashraful Islam Khan, Md Taufiqul Islam, Zahid Hasan Khan, Nabid Anjum Tanvir, Mohammad Ashraful Amin, Ishtiakul Islam Khan, Abu Toha M. R. H. Bhuiyan, A. S. M. Mainul Hasan, Muhammad Shariful Islam, Tajul Islam Abdul Bari, Aninda Rahman, Md. Nazmul Islam and Firdausi Qadri
Vaccines 2023, 11(4), 843; https://doi.org/10.3390/vaccines11040843 - 14 Apr 2023
Cited by 6 | Viewed by 3163
Abstract
Background: Over 700,000 Myanmar nationals known as the ‘Rohingyas’ fled into Cox’s Bazar, Bangladesh, in late 2017. Due to this huge displacement into unhygienic areas, these people became vulnerable to communicable diseases including cholera. Assessing the risk, the Government of Bangladesh (GoB), with [...] Read more.
Background: Over 700,000 Myanmar nationals known as the ‘Rohingyas’ fled into Cox’s Bazar, Bangladesh, in late 2017. Due to this huge displacement into unhygienic areas, these people became vulnerable to communicable diseases including cholera. Assessing the risk, the Government of Bangladesh (GoB), with the help of the International Centre for Diarrhoeal Disease Research, Bangladesh (ICDDR,B) and other international partners, decided to take preventive measures, one of which is the execution of oral cholera vaccination (OCV) campaigns. This paper describes the implementation and delivery of OCV campaigns during humanitarian crises in Bangladesh. Methods: Seven rounds of OCV campaigns were conducted between October 2017 and December 2021. The OCV campaigns were conducted by applying different strategies. Results: Approximately 900,000 Rohingya Myanmar nationals (RMNs) and the host population (amounting to 528,297) received OCV across seven campaigns. In total, 4,661,187 doses of OCVs were administered, which included 765,499 doses for RMNs, and 895,688 doses for the host community. The vaccine was well accepted, and as a result, a high level of coverage was achieved, ranging from 87% to 108% in different campaigns. Conclusions: After successful pre-emptive campaigns in Cox’s Bazar humanitarian camps, no cholera outbreaks were detected either in the RMN or host communities. Full article
(This article belongs to the Section Vaccines and Public Health)
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9 pages, 265 KiB  
Article
Anticardiolipin Antibodies in Patients with Cancer: A Case–Control Study
by Md. Ashraful Islam Nipu, Shoumik Kundu, Sayeda Sadia Alam, Ashrafun Naher Dina, Md. Ashraful Hasan, Mohammad Khan, Md. Ibrahim Khalil, Tareq Hossan and Md Asiful Islam
Cancers 2023, 15(7), 2087; https://doi.org/10.3390/cancers15072087 - 31 Mar 2023
Cited by 2 | Viewed by 2589
Abstract
Antiphospholipid antibodies are highly prevalent in autoimmune diseases and mainly associated with thromboembolic events, which is one of the major reasons for cancer-related mortality. Confirmed adult cancer patients were included (n = 40) with an equal number of age- and sex-matched healthy controls. [...] Read more.
Antiphospholipid antibodies are highly prevalent in autoimmune diseases and mainly associated with thromboembolic events, which is one of the major reasons for cancer-related mortality. Confirmed adult cancer patients were included (n = 40) with an equal number of age- and sex-matched healthy controls. The presence and concentration of anticardiolipin antibodies were investigated by the enzyme-linked immunosorbent assay using the venous blood samples. aCL antibodies were detected in 60.0% (n = 24) of the cancer patients compared to none in the healthy controls (p < 0.001). The serum concentration of aCL antibodies was significantly higher in cancer patients than controls (p < 0.001) and ranged from 89.0 U/mL to 133.0 U/mL among the aCL-positive patients. All the lung cancer patients (n = 6) were diagnosed with positive aCL, and a borderline significant association of aCL antibody positivity was observed in colon cancer patients (p = 0.051). About 72.7% of the advanced-stage cancer individuals and 81.8% of the cancer patients who underwent surgery were diagnosed with positive aCL antibodies. A significant association of aCL antibody positivity was observed with cancer patients comorbid with heart diseases (p = 0.005). The prevalence and serum levels of aCL antibodies were significantly higher in cancer patients compared to healthy controls. Cancer patients (i.e., lung, liver, and colon), at advanced-stage, comorbid with heart diseases, who underwent surgery, were more likely to be diagnosed with aCL antibodies. Full article
14 pages, 1214 KiB  
Article
Measuring the Effectiveness of COVID-19 Vaccines Used during a Surge of the Delta Variant of SARS-CoV-2 in Bangladesh: A Test-Negative Design Evaluation
by Farhana Khanam, Md Taufiqul Islam, Faisal Ahmmed, Shams Uddin Ahmed, Md Ismail Hossen, MdNazmul Hasan Rajib, Shahinur Haque, Prasanta Kumar Biswas, Imam Tauheed, K Zaman, Ahmed Nawsher Alam, Mallick Masum Billah, Monalisa, Shah Ali Akbar Ashrafi, Mohammed Ziaur Rahman, Omar Hamza Bin Manjur, Mokibul Hassan Afrad, S M Shamsuzzaman, Ahmed Abu Saleh, Mostafa Aziz Sumon, Asif Rashed, Md Taufiqur Rahman Bhuiyan, Fahima Chowdhury, Ashraful Islam Khan, Meerjady Sabrina Flora, Tahmina Shirin, John D. Clemens and Firdausi Qadriadd Show full author list remove Hide full author list
Vaccines 2022, 10(12), 2069; https://doi.org/10.3390/vaccines10122069 - 2 Dec 2022
Cited by 7 | Viewed by 3870
Abstract
Background: From May to December 2021, Bangladesh experienced a major surge in the Delta variant of SARS-CoV-2. The earlier rollout of several vaccines offered the opportunity to evaluate vaccine effectiveness against this variant. Methods: A prospective, test-negative case-control study was conducted in five [...] Read more.
Background: From May to December 2021, Bangladesh experienced a major surge in the Delta variant of SARS-CoV-2. The earlier rollout of several vaccines offered the opportunity to evaluate vaccine effectiveness against this variant. Methods: A prospective, test-negative case-control study was conducted in five large hospitals in Dhaka between September and December 2021. The subjects were patients of at least 18 years of age who presented themselves for care, suffering COVID-like symptoms of less than 10 days’ duration. The cases had PCR-confirmed infections with SARS-CoV-2, and up to 4 PCR test-negative controls were matched to each case, according to hospital, date of presentation, and age. Vaccine protection was assessed as being the association between the receipt of a complete course of vaccine and the occurrence of SARS-CoV-2 disease, with symptoms beginning at least 14 days after the final vaccine dose. Results: In total, 313 cases were matched to 1196 controls. The genotyping of case isolates revealed 99.6% to be the Delta variant. Receipt of any vaccine was associated with 12% (95% CI: −21 to 37, p = 0.423) protection against all episodes of SARS-CoV-2. Among the three vaccines for which protection was evaluable (Moderna (mRNA-1273); Sinopharm (Vero Cell-Inactivated); Serum Institute of India (ChAdOx1 nCoV-19)), only the Moderna vaccine was associated with significant protection (64%; 95% CI: 10 to 86, p = 0.029). Protection by the receipt of any vaccine against severe disease was 85% (95% CI: 27 to 97, p = 0.019), with protection estimates of 75% to 100% for the three vaccines. Conclusions: Vaccine protection against COVID-19 disease of any severity caused by the Delta variant was modest in magnitude and significant for only one of the three evaluable vaccines. In contrast, protection against severe disease was high in magnitude and consistent for all three vaccines. Because our findings are not in complete accord with evaluations of the same vaccines in more affluent settings, our study underscores the need for country-level COVID-19 vaccine evaluations in developing countries. Full article
(This article belongs to the Section COVID-19 Vaccines and Vaccination)
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33 pages, 3966 KiB  
Review
Tissue Culture in Ornamentals: Cultivation Factors, Propagation Techniques, and Its Application
by Hasan Mehbub, Ayasha Akter, Mst. Arjina Akter, Mohammad Shamim Hasan Mandal, Md. Ashraful Hoque, Monika Tuleja and Hasan Mehraj
Plants 2022, 11(23), 3208; https://doi.org/10.3390/plants11233208 - 23 Nov 2022
Cited by 47 | Viewed by 21796
Abstract
Ornamentals come in a variety of shapes, sizes, and colors to suit a wide range of climates, landscapes, and gardening needs. Compared to demand, a shortage of plant materials and diversity force the search for solutions for their constant acquisition and improvement to [...] Read more.
Ornamentals come in a variety of shapes, sizes, and colors to suit a wide range of climates, landscapes, and gardening needs. Compared to demand, a shortage of plant materials and diversity force the search for solutions for their constant acquisition and improvement to increase their commercial value, respectively. In vitro cultures are a suitable solution to meet expectations using callus culture, somatic embryogenesis, protoplast culture, and the organogenesis of protocorm-like bodies; many of these techniques are commercially practiced. Factors such as culture media, explants, carbohydrates, plant growth regulators, and light are associated with the success of in vitro propagation. Techniques, especially embryo rescue and somatic hybridization, are widely used to improve ornamentals. The development of synthetic seed allows season-independent seed production and preservation in the long term. Despite the advantages of propagation and the improvement of ornamentals, many barriers still need to be resolved. In contrast to propagation and crop developmental studies, there is also a high scope for molecular studies, especially epigenetic changes caused by plant tissue culture of ornamentals. In this review, we have accumulated and discussed an overall update on cultivation factors, propagation techniques in ornamental plant tissue culture, in vitro plant improvement techniques, and future perspectives. Full article
(This article belongs to the Special Issue New Insight into Research in In Vitro Plants Propagation)
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18 pages, 5868 KiB  
Article
Dual Band Antenna Design and Prediction of Resonance Frequency Using Machine Learning Approaches
by Md. Ashraful Haque, Nayan Sarker, Narinderjit Singh Sawaran Singh, Md Afzalur Rahman, Md. Nahid Hasan, Mirajul Islam, Mohd Azman Zakariya, Liton Chandra Paul, Adiba Haque Sharker, Ghulam E. Mustafa Abro, Md Hannan and Ripon Pk
Appl. Sci. 2022, 12(20), 10505; https://doi.org/10.3390/app122010505 - 18 Oct 2022
Cited by 53 | Viewed by 7637
Abstract
An inset fed-microstrip patch antenna (MPA) with a partial ground structure is constructed and evaluated in this paper. This article covers how to evaluate the performance of the designed antenna by using a combination of simulation, measurement, creation of the RLC equivalent circuit [...] Read more.
An inset fed-microstrip patch antenna (MPA) with a partial ground structure is constructed and evaluated in this paper. This article covers how to evaluate the performance of the designed antenna by using a combination of simulation, measurement, creation of the RLC equivalent circuit model, and the implementation of machine learning approaches. The MPA’s measured frequency range is 7.9–14.6 GHz, while its simulated frequency range is 8.35–14.25 GHz in CST microwave studio (CST MWS) 2018. The measured and simulated bandwidths are 6.7 GHz and 5.9 GHz, respectively. The antenna substrate is composed of FR-4 Epoxy, which has a dielectric constant of 4.4 and a loss tangent of 0.02. The equivalent model of the proposed MPA is developed by using an advanced design system (ADS) to compare the resonance frequencies obtained by using CST. In addition, the measured return loss of the prototype is compared with the simulated return loss observed by using CST and ADS. At the end, 86 data samples are gathered through the simulation by using CST MWS, and seven machine learning (ML) approaches, such as convolutional neural network (CNN), linear regression (LR), random forest regression (RFR), decision tree regression (DTR), lasso regression, ridge regression, and extreme gradient boosting (XGB) regression, are applied to estimate the resonant frequency of the patch antenna. The performance of the seven ML models is evaluated based on mean square error (MSE), mean absolute error (MAE), root mean square error (RMSE), and variance score. Among the seven ML models, the prediction result of DTR (MSE = 0.71%, MAE = 5.63%, RMSE = 8.42%, and var score = 99.68%) is superior to other ML models. In conclusion, the proposed antenna is a strong contender for operating at the entire X-band and lower portion of the Ku-band frequencies, as evidenced by the simulation results through CST and ADS, it measured and predicted results using machine learning approaches. Full article
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14 pages, 2249 KiB  
Article
In Vitro Screening and Field Performance of EMS-Treated Eggplants for the Selection of Shoot and Fruit Borer-Resistant Plants
by Md Ashraful Islam, Md Muntasir Bin Mohi Uddin, Md Golam Rasul, Md Ahsanul Haque Swapon, Minhaz Ahmed and Mehfuz Hasan
Agronomy 2022, 12(8), 1832; https://doi.org/10.3390/agronomy12081832 - 2 Aug 2022
Cited by 5 | Viewed by 2952
Abstract
The shoot and fruit borer has asserted itself as a severe pest of eggplant. In vitro mutagenesis is an excellent method for producing mutants resistant to biotic and abiotic stresses. This study aimed to create eggplant mutants that were resistant to shoot and [...] Read more.
The shoot and fruit borer has asserted itself as a severe pest of eggplant. In vitro mutagenesis is an excellent method for producing mutants resistant to biotic and abiotic stresses. This study aimed to create eggplant mutants that were resistant to shoot and fruit borer infestation. In the Murashige and Skoog (MS) medium, two genotypes, including a landrace, were treated with varying amounts of EMS. Among the treatments, MS medium supplemented with 2% EMS demonstrated the most favorable effect. Explant performance dropped by up to 1.5% with an increase in EMS in the medium. The 2% EMS dose was appropriate for in vitro mutant eggplant development, but the higher dose was extremely damaging. EMS made several mutants sterile. Three landrace mutants were insect-resistant. Total phenols, leaf chlorophylls A and B, antioxidants, and solasodine were abundant in these genotypes. Correlation studies found a link between affected fruits and shoots and total phenols, antioxidants, and solasodine. Solasodine was shown to be related to chlorophylls A and B. The eggplant shoot and fruit borer infestation rate can be reduced by increasing these specific biochemical properties. Full article
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10 pages, 507 KiB  
Article
Plasma Kynurenine to Tryptophan Ratio Is Not Associated with Undernutrition in Adults but Reduced after Nutrition Intervention: Results from a Community-Based Study in Bangladesh
by Md. Amran Gazi, Md. Abdullah Siddique, Md. Ashraful Alam, Farzana Hossaini, Md. Mehedi Hasan, Shah Mohammad Fahim, Barbie Zaman Wahid, Md. Mamun Kabir, Subhasish Das, Mustafa Mahfuz and Tahmeed Ahmed
Nutrients 2022, 14(9), 1708; https://doi.org/10.3390/nu14091708 - 20 Apr 2022
Cited by 4 | Viewed by 2527
Abstract
Infections and persistent immunological activation are linked to increased kynurenine (KYN) and the KYN-to-Tryptophan (TRP) or KT ratio and may be critical factors in undernutrition. We sought to determine the association between the KT ratio and adult malnutrition, as well as investigate if [...] Read more.
Infections and persistent immunological activation are linked to increased kynurenine (KYN) and the KYN-to-Tryptophan (TRP) or KT ratio and may be critical factors in undernutrition. We sought to determine the association between the KT ratio and adult malnutrition, as well as investigate if nutritional supplementation had any influence on the decrease of the KT ratio. A total of 525 undernourished adults aged 18–45 years were recruited and provided a nutrition intervention for 60 feeding days. TRP and KYN concentrations were determined from plasma samples using LC-MS/MS. At baseline, the median (interquartile range (IQR)) TRP, KYN and KT ratios were 24.1 (17.6, 34.3) µmol/L, 0.76 (0.53, 1.18) µmol/L and 30.9 (24.5, 41.7), respectively. Following intervention, the median (IQR) KYN and KT ratios were significantly reduced to 0.713 (0.46, 1.12) µmol/L and 27.5 (21.3, 35.8). The KT ratio was found to be inversely linked with adult BMI (coefficient: −0.09; 95% CI: −0.18, 0.004; p-value = 0.06) but not statistically significant. Additionally, Plasma CRP was correlated positively, while LRP1 was inversely correlated with the KT ratio. Our data suggest that in Bangladeshi adults, the KT ratio is not related to the pathophysiology of malnutrition but correlated with inflammatory and anti-inflammatory biomarkers, and the ratio can be reduced by a nutrition intervention. Full article
(This article belongs to the Section Nutritional Epidemiology)
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15 pages, 2173 KiB  
Article
Exploratory Analysis of Selected Components of the mTOR Pathway Reveals Potentially Crucial Associations with Childhood Malnutrition
by Parag Palit, Md Amran Gazi, Subhasish Das, Md Mehedi Hasan, Zannatun Noor, Jafrin Ferdous, Md Ashraful Alam, Sharika Nuzhat, Md Ridwan Islam, Mustafa Mahfuz, Rashidul Haque and Tahmeed Ahmed
Nutrients 2022, 14(8), 1612; https://doi.org/10.3390/nu14081612 - 12 Apr 2022
Cited by 4 | Viewed by 2725
Abstract
Dysregulations in the mammalian target of rapamycin (mTOR) pathway are associated with several human anomalies. We aimed to elucidate possible implications for potential aberrations in the mTOR pathway with childhood malnutrition. We analyzed the activity of phospho-mTORC1 and the expressions of several mTOR [...] Read more.
Dysregulations in the mammalian target of rapamycin (mTOR) pathway are associated with several human anomalies. We aimed to elucidate possible implications for potential aberrations in the mTOR pathway with childhood malnutrition. We analyzed the activity of phospho-mTORC1 and the expressions of several mTOR pathway genes, namely: MTOR, TSC1, LAMTOR2, RPS6K1 and RICTOR from peripheral blood mononuclear cells isolated from venous blood of children suffering from different forms of malnutrition and compared them with those from healthy children. Significant reduction in the phosphorylation of mTORC1 was noted, as well as a decrease in expression of LAMTOR2 gene and increase in TSC1 gene expression were observed between malnourished children in comparison to the healthy children. The deregulation in the activity of the TSC1 and LAMTOR2 gene was significantly associated with all forms of childhood malnutrition. Our findings provide key insights into possible down-modulation in the overall activity of the mTOR pathway in childhood malnutrition. Further studies focusing on the analysis of a multitude of components involved in the mTOR pathway both at the gene and protein expression levels are required for conclusive evidence for the aforementioned proposition. Full article
(This article belongs to the Section Pediatric Nutrition)
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14 pages, 2172 KiB  
Article
Effects of Organic Amendments on Soil Aggregate Stability, Carbon Sequestration, and Energy Use Efficiency in Wetland Paddy Cultivation
by Mohammad Mizanur Rahman, Mohammed Zia Uddin Kamal, Senaratne Ranamukhaarachchi, Mohammad Saiful Alam, Mohammad Khairul Alam, Mohammad Arifur Rahman Khan, Mohammad Moshiul Islam, Mohammad Ashraful Alam, Saidul Islam Jiban, Mohammad Abdullah Al Mamun, Hasan Muhammad Abdullah, Jatish Chandra Biswas, Sohela Akhter, Umme Aminun Naher, Md. Maniruzzaman, Mohammad Mozammel Haque and Faruque Ahmed
Sustainability 2022, 14(8), 4475; https://doi.org/10.3390/su14084475 - 9 Apr 2022
Cited by 26 | Viewed by 3939
Abstract
A study was conducted to assess the effects of organic amendments on soil aggregates, carbon (C) sequestration, and energy use efficiency (EUE) during five consecutive Boro and Transplanted Aman rice seasons in Bangladesh during 2018–2020. Five treatments (viz., control (only inorganic fertilizers), cow [...] Read more.
A study was conducted to assess the effects of organic amendments on soil aggregates, carbon (C) sequestration, and energy use efficiency (EUE) during five consecutive Boro and Transplanted Aman rice seasons in Bangladesh during 2018–2020. Five treatments (viz., control (only inorganic fertilizers), cow dung (CD), vermicompost (VC), rice straw (RS), and poultry manure (PM)) were used. The organic materials were applied at 2 t C ha−1 season−1 to all the plots, except in the control treatment. Inorganic fertilizers were applied in all treatments in both seasons following integrated nutrient management (INM). The data reveal that PM was found to be more efficient at increasing the water-stable soil aggregates (WSA), followed by the RS, CD, and VC. The WSA in smaller-sized soil aggregates were found to be higher than those in larger-sized soil aggregates. VC was found to be the most effective in terms of C sequestration (29%), followed by PM (26%), CD (22%), and RS (20%). The highest EUE was attributed to the control treatment (9.77), followed by the CD (8.67), VC (8.04), RS (2.10), and PM (1.18), which showed energy wastage in the organic treatments. The system productivity (SP) followed the opposite trend of the EUE. The INM is a better approach to improve the soil health, the C sequestration, and the SP, but it appeared as an energy-inefficient strategy, which suggests that a balanced application of organic and inorganic nutrients is needed in order to achieve yield sustainability and EUE. Full article
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19 pages, 951 KiB  
Article
Determinants of COVID-19 Vaccine Acceptance among the Adult Population of Bangladesh Using the Health Belief Model and the Theory of Planned Behavior Model
by Muhammad Mainuddin Patwary, Mondira Bardhan, Asma Safia Disha, Mehedi Hasan, Md. Zahidul Haque, Rabeya Sultana, Md. Riad Hossain, Matthew H. E. M. Browning, Md. Ashraful Alam and Malik Sallam
Vaccines 2021, 9(12), 1393; https://doi.org/10.3390/vaccines9121393 - 25 Nov 2021
Cited by 91 | Viewed by 14811
Abstract
Vaccination is undoubtedly one of the most effective strategies to halt the COVID-19 pandemic. The current study aimed to investigate the acceptance of COVID-19 vaccination and its associated factors using two health behavior change frameworks: the Health Belief Model (HBM) and the Theory [...] Read more.
Vaccination is undoubtedly one of the most effective strategies to halt the COVID-19 pandemic. The current study aimed to investigate the acceptance of COVID-19 vaccination and its associated factors using two health behavior change frameworks: the Health Belief Model (HBM) and the Theory of Planned Behavior (TPB). A total of 639 Bangladeshi adults (mean age: 24 years) participated in a cross-sectional online study between July and August 2021. The questionnaire covered questions regarding vaccine intentions, sociodemographic features, health status, perceived trust in/satisfaction with health authorities, reasons for vaccine hesitancy, and factors related to the health behavior change frameworks. Hierarchical logistic regression was employed to determine associations between these predictors and vaccine acceptance. The intention to get a COVID-19 vaccination was expressed among 85% of the participants. In fully adjusted models, students and respondents with more normal body weights reported higher intentions to get vaccinated. Respondents were also more likely to seek vaccination if they reported greater levels of perceived susceptibility, benefits, and cues to action, as well as lower levels of barriers and self-efficacy. Fear of future vaccine side effects was the most common reason for COVID-19 vaccine hesitancy and was expressed by 94% of the vaccine-hesitant respondents. These factors should be considered by health authorities in Bangladesh and perhaps other countries when addressing the plateauing COVID-19 vaccination rates in many populations. Full article
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22 pages, 2055 KiB  
Review
Using Chitosan or Chitosan Derivatives in Cancer Therapy
by Md Salman Shakil, Kazi Mustafa Mahmud, Mohammad Sayem, Mahruba Sultana Niloy, Sajal Kumar Halder, Md. Sakib Hossen, Md. Forhad Uddin and Md. Ashraful Hasan
Polysaccharides 2021, 2(4), 795-816; https://doi.org/10.3390/polysaccharides2040048 - 13 Oct 2021
Cited by 63 | Viewed by 8952
Abstract
Cancer is one of the major causes of death worldwide. Chemotherapeutic drugs have become a popular choice as anticancer agents. Despite the therapeutic benefits of chemotherapeutic drugs, patients often experience side effects and drug resistance. Biopolymers could be used to overcome some of [...] Read more.
Cancer is one of the major causes of death worldwide. Chemotherapeutic drugs have become a popular choice as anticancer agents. Despite the therapeutic benefits of chemotherapeutic drugs, patients often experience side effects and drug resistance. Biopolymers could be used to overcome some of the limitations of chemotherapeutic drugs, as well as be used either as anticancer agents or drug delivery vehicles. Chitosan is a biocompatible polymer derived from chitin. Chitosan, chitosan derivatives, or chitosan nanoparticles have shown their promise as an anticancer agent. Additionally, functionally modified chitosan can be used to deliver nucleic acids, chemotherapeutic drugs, and anticancer agents. More importantly, chitosan-based drug delivery systems improved the efficacy, potency, cytotoxicity, or biocompatibility of these anticancer agents. In this review, we will investigate the properties of chitosan and chemically tuned chitosan derivatives, and their application in cancer therapy. Full article
(This article belongs to the Collection Current Opinion in Polysaccharides)
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