Molecular Characterization of Various Viruses and Bacteria in Small Animals

A special issue of Veterinary Sciences (ISSN 2306-7381). This special issue belongs to the section "Veterinary Microbiology, Parasitology and Immunology".

Deadline for manuscript submissions: 15 October 2025 | Viewed by 1797

Special Issue Editors


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Guest Editor
Schreiber School of Veterinary Medicine, Rowan University, Glassboro, NJ, USA
Interests: immunology; clinical microbiology; molecular biology; bioinformatics

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Guest Editor
Department of Clinical Sciences, College of Veterinary Medicine, Kansas State University, Manhattan, KS 66506, USA
Interests: antimicrobial resistance in gut bacteria of cattle and swine
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Special Issue Information

Dear Colleagues,

With new sequencing technologies becoming more accessible to researchers and organizations worldwide, the identification of novel and emerging viruses and bacteria has significantly improved. We are excited to invite you to submit your manuscripts to our Special Issue on the “Molecular Characterization of Various Viruses and Bacteria in Small Animals,” to be published in the MDPI journal Veterinary Sciences. This Special Issue aims to showcase innovative research utilizing advanced sequencing technologies and bioinformatics to unravel the complexities of pathogens affecting small animals.

We seek original research articles and reviews that explore the use of long-read and short-read sequencing platforms in characterizing pathogens. Studies that integrate these sequencing data for comprehensive pathogen analysis are particularly welcome.

Additionally, we are interested in bioinformatics pipelines that analyze viral and bacterial genomes in small animals, including metagenomic sequencing, targeted next-generation sequencing, and the analysis of bacterial communities. We encourage submissions that focus on the assembly and annotation of viral genomes, comparative genomics of bacterial pathogens, and the development and validation of molecular diagnostics, including CRISPR-based tests targeting these microbes in small animals.

We also seek research on the detection of antimicrobial resistance genes, virulence genes, and temporal changes in these genes. Innovations in hybrid sequencing approaches, bioinformatics tools for viral quasispecies analysis, and machine learning applications in bioinformatics analysis of sequence data are highly encouraged.

Your contributions will play a crucial role in advancing our understanding of pathogen biology and host–pathogen interactions, fostering a One Health approach to combating infectious diseases. This Special Issue provides a unique platform to highlight your research to a global audience and to contribute to a growing body of knowledge that bridges the veterinary and human health sciences.

Please submit your manuscripts through the MDPI submission system by 7th March 2025. We look forward to receiving your valuable contributions and working together to advance this important field.

Dr. Mohamed Abouelkhair
Dr. Raghavendra Amachawadi
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Veterinary Sciences is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2100 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • molecular characterization
  • small animal pathogens
  • advanced sequencing technologies
  • bioinformatics
  • antimicrobial resistance

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Published Papers (1 paper)

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Research

14 pages, 4598 KiB  
Article
Genetic and Phylogenetic Analysis of Feline Coronavirus in Guangxi Province of China from 2021 to 2024
by Kaichuang Shi, Mengyi He, Yuwen Shi, Feng Long, Yandi Shi, Yanwen Yin, Yi Pan, Zongqiang Li and Shuping Feng
Vet. Sci. 2024, 11(10), 455; https://doi.org/10.3390/vetsci11100455 - 25 Sep 2024
Viewed by 1193
Abstract
Feline coronavirus (FCoV), as one of the important pathogens of feline viral gastroenteritis, has been attracting great attention. A total of 1869 rectal and nasal swabs, feces, and ascites samples were collected from eight regions in Guangxi province during 2021–2024. The multiplex RT-qPCR [...] Read more.
Feline coronavirus (FCoV), as one of the important pathogens of feline viral gastroenteritis, has been attracting great attention. A total of 1869 rectal and nasal swabs, feces, and ascites samples were collected from eight regions in Guangxi province during 2021–2024. The multiplex RT-qPCR established in our laboratory was used to test these samples for FCoV, and 17.66% (330/1869) of the samples were positive for FCoV. The S, M, and N genes of 63 FCoV-positive samples were amplified and sequenced, and the genetic and evolutionary characteristics were analyzed. Similarity analysis showed that the nucleotide and amino acid homologies of S, M, and N genes were 81.2–99.6% and 70.2–99.5%, 89.9–100% and 91.6–100%, and 90.1–100% and 91.5–100%, respectively. Phylogenetic analysis revealed that all 63 FCoV strains, based on S gene sequences, belonged to type I FCoV (FCoV-I), and were clustered with Chinese strains and the Netherlands UU strains. Recombinant signals were detected in the S gene of strains GXLZ03-2022, GXLZ08-2022, and CCoV GD/2020/X9. The results suggest that FCoV is still prevalent in the Guangxi province of southern China, and the prevalent FCoV strains show high genetic diversity and novel epidemic characteristics. Full article
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