Advances in Small Animal Reproduction: Clinical, Molecular and Biotechnological Perspectives

A Special Issue of Veterinary Sciences (ISSN 2306-7381) belonging to the section "Veterinary Reproduction and Obstetrics".

Deadline for manuscript submissions: 30 November 2026 | Viewed by 989

Editor


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Guest Editor
Faculty of Veterinary Medicine, Trakia University, 6000 Stara Zagora, Bulgaria
Interests: contraception in small animals; pregnancy pathology; hormone therapy

Special Issue Information

Dear Colleagues,

Small animal reproduction is a rapidly evolving field, driven by the need for advanced clinical management and companion animal welfare. This Special Issue, "Advances in Small Animal Reproduction: Clinical, Molecular and Biotechnological Perspectives", provides a comprehensive platform for the latest developments in canine and feline theriogenology. We invite original research and comprehensive reviews that explore novel clinical strategies. A special emphasis is placed on revisiting traditional paradigms of hormonal therapy through a modern lens—focusing on their refinement, alternative routes of drug administration and optimized dosing regimens. Furthermore, we encourage submissions on estrous cycle regulation, non-surgical contraception, the prevention of reproductive disorders and the impact of climate and climate change on the estrous cycle.

To reflect the multidisciplinary nature of veterinary medicine, this issue also highlights cutting-edge reproductive biotechnologies and molecular insights into the physiopathology of the reproductive tract.

Above all, we seek to foster the bold expression of hypotheses. By welcoming daring, hypothesis-driven research, this Special Issue aims to spark a new beginning for future innovations, successfully bridging the gap between basic science and advanced veterinary clinical practice.

Prof. Dr. Plamen Ivanchev Georgiev
Guest Editor

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Keywords

  • canine and feline theriogenology
  • hormonal therapy and prevention
  • estrous cycle regulation
  • contraception
  • climate change

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Published Papers (1 paper)

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Research

20 pages, 1736 KB  
Article
Comparative Evaluation of Staining Techniques in Thawed Cryopreserved Dog Semen
by Indra Sara Klumb, Axel Wehrend and Abbas Farshad
Vet. Sci. 2026, 13(7), 627; https://doi.org/10.3390/vetsci13070627 - 27 Jun 2026
Viewed by 320
Abstract
Background: Accurate assessment of sperm morphology is essential for evaluating the quality of cryopreserved canine semen used in artificial insemination and for improving cryopreservation protocols. This study compared six staining techniques, Eosin, Eosin–Nigrosin, Diff-Quick®, Hemacolor®, Spermac®, and [...] Read more.
Background: Accurate assessment of sperm morphology is essential for evaluating the quality of cryopreserved canine semen used in artificial insemination and for improving cryopreservation protocols. This study compared six staining techniques, Eosin, Eosin–Nigrosin, Diff-Quick®, Hemacolor®, Spermac®, and Formol-citrate Bengal Rose, for light-microscopic evaluation of frozen–thawed canine spermatozoa. Methods: Semen from ten dogs was thawed, divided into four aliquots, and either left untreated or exposed to thermal stress at 6 °C, 18 °C, or 37 °C for two hours to induce morphological variation. A total of 360 slides and 960 evaluations were performed immediately after staining and again after 24 h, 7 days, and 3 months to assess staining quality and stability over time. Results: Eosin produced stable staining for up to three months and was the most economical method, though its initial detail recognition was lower than that of Spermac® and Formol-citrate Bengal Rose. Eosin–Nigrosin showed reduced contrast and detail. Diff-Quick® provided better contrast than Eosin–Nigrosin, while Hemacolor® maintained consistent quality regardless of stress treatment or storage duration. Spermac® yielded the highest initial morphological detail but deteriorated during storage. Formol-citrate Bengal Rose combined high detail recognition with stable staining throughout the study. Cryopreservation increased looped tails, and incubation at 37 °C markedly elevated pathological sperm and rudimentary tails. Conclusions: All six staining methods were suitable for evaluating and archiving frozen–thawed canine semen. Formol-citrate Bengal Rose and Spermac® offered the best detail, while Eosin provided a cost-effective option with excellent long-term stability. Full article
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