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Special Issue "Characterization and Quantitative Analysis of Botulinum Neurotoxin"

A special issue of Toxins (ISSN 2072-6651). This special issue belongs to the section "Bacterial Toxins".

Deadline for manuscript submissions: 31 July 2019

Special Issue Editor

Guest Editor
Dr. Suzanne R. Kalb

Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy, NE, Atlanta, GA 30341, USA
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Special Issue Information

Dear Colleagues,

Botulism is caused by exposure to Botulinum neurotoxins (BoNTs), protein toxins produced by Clostridium botulinum and related Clostridia. With seven confirmed serotypes and over 40 subtypes, the diversity of these neurotoxins is both fascinating and challenging. Characterization of the varied BoNTs through qualitative analysis is critical for understanding toxicity/mechanism of action, and structure/function relationships, designing medical countermeasures to treat or prevent botulism, and development of assays to detect the neurotoxin. A quantitiative analysis of the neurotoxin assists in medical countermeasure development as well as toxicity assessments. This Special Issue will focus on qualitative and/or quantitative analysis of BoNTs as it applies to mechanism of action, toxicity assessment, structure/function relationships, medical countermeasure development, and assay development.

Dr. Suzanne R. Kalb
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a double-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Toxins is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Botulinum neurotoxin
  • Botulism
  • Mechanism of action
  • Toxicity assessment
  • Structure/function relationship
  • Quantitative analysis

Published Papers (1 paper)

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Research

Open AccessArticle Content/Potency Assessment of Botulinum Neurotoxin Type-A by Validated Liquid Chromatography Methods and Bioassays
Received: 22 November 2018 / Revised: 28 December 2018 / Accepted: 10 January 2019 / Published: 12 January 2019
PDF Full-text (756 KB) | HTML Full-text | XML Full-text
Abstract
Botulinum neurotoxin type-A (BoNTA) is one of the seven different serotypes (A to G) produced by Clostridium botulinum. A stability-indicating size-exclusion chromatography (SEC) method was developed and validated, and the specificity was confirmed by forced degradation study, interference of the excipients, and peaks [...] Read more.
Botulinum neurotoxin type-A (BoNTA) is one of the seven different serotypes (A to G) produced by Clostridium botulinum. A stability-indicating size-exclusion chromatography (SEC) method was developed and validated, and the specificity was confirmed by forced degradation study, interference of the excipients, and peaks purity. The method was applied to assess the content and high-molecular-weight (HMW) forms of BoNTA in biopharmaceutical products, and the results were compared with those of the LD50 mouse bioassay, the T−47D cell culture assay, and the reversed-phase chromatography (RPC) method, giving mean values of 0.71% higher, 0.36% lower, and 0.87% higher, respectively. Aggregated forms showed significant effects on cytotoxicity, as well as a decrease in the bioactivity (p < 0.05). The employment of the proposed method in conjunction with the optimized analytical technologies for the analysis of the intact and altered forms of the biotechnology-derived medicines, in the correlation studies, enabled the demonstration of the capability of each one of the methods and allowed for great improvements, thereby assuring their safe and effective use. Full article
(This article belongs to the Special Issue Characterization and Quantitative Analysis of Botulinum Neurotoxin)
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