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Symmetry
Special Issues
Molecular Biology and Genome Analysis
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Molecular Biology and Genome Analysis

A special issue of Symmetry (ISSN 2073-8994). This special issue belongs to the section "Life Sciences".

Deadline for manuscript submissions: closed (31 July 2021) | Viewed by 2436

Special Issue Editor

Dr. Yoshihito Kishita

E-Mail Website
Guest Editor
Laboratory of Genome Sciences, Department of Life Science, Faculty of Science and Engineering, Kindai University, 3 Chome-4-1 Kowakae, Higashiosaka, Osaka 577-8502, Japan
Interests: human genetics; molecular biology; genetic disease; mitochondria

Special Issue Information

Dear Colleagues,

The development of genome analysis technology has been remarkable, and the technological progress of next-generation sequencing is constantly advancing. By targeting DNA, RNA, and epigenetic changes to genome analysis, not only the genome sequence but also gene expression and regulation can be revealed. These sequencing technologies have led to the elucidation of the causes of various diseases in the clinical research field, but also in basic research, the genomes of various species have been revealed, and the evolutionary background has been clarified. Today, single-cell sequencing technologies are gaining momentum, and genome analysis at the cellular level has become possible. The details of developmental processes and tissue formation involving symmetry and asymmetry have been revealed by this technology. Single-cell sequencing is increasingly being applied to the medical field, particularly to reveal pathological conditions at the cellular level.

The scope of this Special Issue will cover discoveries in molecular biology revealed by extensive genome analysis research. In particular, we would like to accept biological phenomena and pathogenic mechanisms of diseases that have been revealed through comprehensive genome analysis. In addition, proposals for new genome analysis technologies and technological improvements are also acceptable.

Dr. Yoshihito Kishita
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Symmetry is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Genome analysis
  • Molecular biology
  • Variant
  • Chromosome
  • Epigenetics
  • Gene expression
  • Genetic disease
  • Evolution
  • Development

Published Papers (1 paper)

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Research

15 pages, 1447 KiB  
Article
Microsatellites as Agents of Adaptive Change: An RNA-Seq-Based Comparative Study of Transcriptomes from Five Helianthus Species
by Chathurani Ranathunge, Sreepriya Pramod, Sébastien Renaut, Gregory L. Wheeler, Andy D. Perkins, Loren H. Rieseberg and Mark E. Welch
Symmetry 2021, 13(6), 933; https://doi.org/10.3390/sym13060933 - 24 May 2021
Cited by 7 | Viewed by 2148
Abstract
Mutations that provide environment-dependent selective advantages drive adaptive divergence among species. Many phenotypic differences among related species are more likely to result from gene expression divergence rather than from non-synonymous mutations. In this regard, cis-regulatory mutations play an important part in generating functionally [...] Read more.
Mutations that provide environment-dependent selective advantages drive adaptive divergence among species. Many phenotypic differences among related species are more likely to result from gene expression divergence rather than from non-synonymous mutations. In this regard, cis-regulatory mutations play an important part in generating functionally significant variation. Some proposed mechanisms that explore the role of cis-regulatory mutations in gene expression divergence involve microsatellites. Microsatellites exhibit high mutation rates achieved through symmetric or asymmetric mutation processes and are abundant in both coding and non-coding regions in positions that could influence gene function and products. Here we tested the hypothesis that microsatellites contribute to gene expression divergence among species with 50 individuals from five closely related Helianthus species using an RNA-seq approach. Differential expression analyses of the transcriptomes revealed that genes containing microsatellites in non-coding regions (UTRs and introns) are more likely to be differentially expressed among species when compared to genes with microsatellites in the coding regions and transcripts lacking microsatellites. We detected a greater proportion of shared microsatellites in 5′UTRs and coding regions compared to 3′UTRs and non-coding transcripts among Helianthus spp. Furthermore, allele frequency differences measured by pairwise FST at single nucleotide polymorphisms (SNPs), indicate greater genetic divergence in transcripts containing microsatellites compared to those lacking microsatellites. A gene ontology (GO) analysis revealed that microsatellite-containing differentially expressed genes are significantly enriched for GO terms associated with regulation of transcription and transcription factor activity. Collectively, our study provides compelling evidence to support the role of microsatellites in gene expression divergence. Full article
(This article belongs to the Special Issue Molecular Biology and Genome Analysis)
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