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Separations
Special Issues
HPLC: A Key Tool for Analytical Chemistry
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Special Issue "HPLC: A Key Tool for Analytical Chemistry"

A special issue of Separations (ISSN 2297-8739).

Deadline for manuscript submissions: 30 November 2021.

Special Issue Editor

Dr. Salvatore Barreca
E-Mail Website
SciProfiles
Guest Editor
ARPA Lombardia, UOC Lab Milano, Sede Lab Monza & Brianza, Via Solferino 16, I-20900 Monza, MB, Italy
Interests: analytical chemistry; UHPLC; separation techniques; hyphenated techniques; separated components
Special Issues and Collections in MDPI journals

Special Issue Information

Dear Colleagues,

Over the last twenty years, high-pressure liquid chromatography (HPLC, UHPLC) hyphenated with several different detectors, has become one of the most useful methods worldwide for the separation and analysis of complex mixtures in many scientific fields, especially, analytical chemistry.

The ongoing evolution of HPLC has included advances in components such as stationary phases used in separation columns, green solvents, detection systems, data handling systems, and sample preparation.

This Special Issue of Separations “HPLC- A Key Tool on Analytical Chemistry” will illustrate the latest research and advancements in HPLC/UHPLC technology and applications. 

This Special Issue accepts contributions that cover all research aspects related to analytical chemistry, and several scientific macro-areas can also be included, such as green analytical chemistry, environmental chemistry and pharmacy, chemometrics, and food and nutrition.

Dr. Salvatore Barreca
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Separations is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Analytical chemistry
  • UHPLC
  • Separation techniques
  • Hyphenated techniques
  • Separated components

Published Papers (1 paper)

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Article
Analysis of Selected Mycotoxins in Maize from North-West South Africa Using High Performance Liquid Chromatography (HPLC) and Other Analytical Techniques
by
Theodora Ijeoma Ekwomadu
,
Toluwase Adeseye Dada
,
Stephen Abiola Akinola
,
Nancy Nleya
and
Mulunda Mwanza
Separations 2021, 8(9), 143; https://doi.org/10.3390/separations8090143 - 03 Sep 2021
Viewed by 215
Abstract
Contamination of foods by mycotoxins is linked to various health and economic implications. This study evaluated the incidence of mycotoxins in commercial and small-scale maize and evaluated potential health risks for consumers based on South African and international regulations. The sensitivity/specificity of HPLC [...] Read more.
Contamination of foods by mycotoxins is linked to various health and economic implications. This study evaluated the incidence of mycotoxins in commercial and small-scale maize and evaluated potential health risks for consumers based on South African and international regulations. The sensitivity/specificity of HPLC over other analytical methods used was also ascertained. In total, 100 maize samples were analyzed using immuno-affinity column for extraction and clean-up, thin layer chromatography (TLC), HPLC, and enzyme linked immunosorbent assay (ELISA) for quantification. Results revealed that fumonisinB1 was the most contaminant mycotoxin in both small-scale and commercial samples with incidence rates of 100% and 98.6%, respectively. Aflatoxins contamination occurred at incidences of 26.7% in small-scale and 25.0% in commercial samples. Furthermore, ochratoxin A had high incidence rates of 97.8% and 93.0% and ranged from 3.60–19.44 µg/kg and 1.60–9.89 µg/kg, respectively, in small-scale and commercial samples, while ZEA occurred in 50% and 55% of small-scale and commercial samples, respectively. Results demonstrate that maize, especially from small-scale farmers, may contribute to dietary exposure to mycotoxins. Farmers and consumers should be alerted to the dangers of mycotoxins contamination in maize with resultant health risks. Additionally, HPLC method was also found to be more specific for mycotoxin detection than ELISA. Full article
(This article belongs to the Special Issue HPLC: A Key Tool for Analytical Chemistry)
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