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Fluorescent Sensors for Advanced Applications in Biological and Chemical Detection

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Chemical Sensors".

Deadline for manuscript submissions: closed (5 February 2026) | Viewed by 2955

Special Issue Editor


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Guest Editor
School of Chemistry, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK
Interests: synthesis; fluorescence spectroscopy; coordination chemistry; bioinorganic chemistry; real-time bioimaging via fluorescent sensors; anticancer studies; copper; magnetic resonance imaging
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Special Issue Information

Dear Colleagues,

Fluorescent sensors are at the forefront of innovative technologies for biological and chemical detection, offering unparalleled sensitivity and specificity. These sensors leverage the unique properties of fluorescent molecules to provide detailed insights into a variety of analytes, including biorelevant metal ions and anions, biomolecules, chemical substances, chemical transformation-tracking compounds, cancer microenvironment targets, and environmental pollutants like heavy metal cations and anions. This Special Issue will focus on the advanced applications of fluorescent sensors, highlighting both theoretical developments and practical implementations in biological and chemical contexts.

The Special Issue will encompass a broad range of topics related to the advanced applications of fluorescent sensors, including but not limited to the following:

  • Development of new fluorescent probes;
  • Biosensing applications;
  • Chemical sensing innovations;
  • Fluorescent sensor integration with analytical techniques;
  • Advancements in fluorescent sensor technology;
  • Real-time bioimaging using fluorescent sensors.

Dr. Sellamuthu Anbu
Guest Editor

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fluorescent sensors
  • biological detection
  • chemical detection
  • fluorescent probes
  • biosensing technologies

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Published Papers (2 papers)

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Research

16 pages, 4048 KB  
Article
A Heptamethine Cyanine-Based Near-Infrared Optical Sensor for Copper(II) Detection in Aqueous Solutions and Living Cells
by Ziya Aydin, Bing Yan and Maolin Guo
Sensors 2026, 26(1), 130; https://doi.org/10.3390/s26010130 - 24 Dec 2025
Viewed by 744
Abstract
Copper ions are essential trace elements that play critical roles in redox reactions, signal transduction, energy metabolism, and regulation of the central nervous system. However, excess copper can induce cytotoxicity and contribute to various pathological conditions, highlighting the need for sensitive and selective [...] Read more.
Copper ions are essential trace elements that play critical roles in redox reactions, signal transduction, energy metabolism, and regulation of the central nervous system. However, excess copper can induce cytotoxicity and contribute to various pathological conditions, highlighting the need for sensitive and selective detection methods. We report a novel near-infrared (NIR) optical sensor, IRPhen, based on a heptamethine cyanine scaffold conjugated with a 1,10-phenanthroline Cu2+-binding receptor. IRPhen exhibits strong NIR absorption and emission (Ex: 750 nm, Em: 808 nm), high sensitivity, and good selectivity toward Cu2+ over competing metal ions. Spectroscopic studies revealed a rapid, reversible 1:1 binding interaction with a binding constant of 1.3 × 106 M−1 and a detection limit of 0.286 µM. The probe demonstrated excellent stability across physiological pH ranges and maintained its performance under competitive conditions. Importantly, IRPhen is cell-permeable and capable of detecting dynamic Cu2+ changes in living fibroblast (WS1) cells using confocal microscopy. This sensor design offers a versatile platform for developing NIR optical sensors to study copper homeostasis, elucidating copper-related biological mechanisms, and potentially developing similar NIR probes for other biologically relevant metal ions. Full article
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19 pages, 5456 KB  
Article
One-Step Microwave-Assisted Fabrication of Carbon Dots as Efficient Fluorescent Chemosensors for Hg2+ and Fe3+ Detection
by Rawan H. Alansari, Esraa M. Bakhsh, Lenah R. Altamimi, Kalsoom Akhtar and Sher Bahadar Khan
Sensors 2025, 25(24), 7452; https://doi.org/10.3390/s25247452 - 7 Dec 2025
Cited by 3 | Viewed by 1593
Abstract
Fluorescent carbon dots (CDs) were efficiently synthesized by a one-step microwave-assisted method using diphenylamine as a carbon precursor. The obtained CDs exhibit high stability and strong water solubility. Under UV irradiation, these CDs could emit bright green photoluminescence. These synthesized CDs have an [...] Read more.
Fluorescent carbon dots (CDs) were efficiently synthesized by a one-step microwave-assisted method using diphenylamine as a carbon precursor. The obtained CDs exhibit high stability and strong water solubility. Under UV irradiation, these CDs could emit bright green photoluminescence. These synthesized CDs have an average diameter of 1.8 nm (±0.46) and quantum yield (QY) as high as 44.69% using rhodamine-B as a reference. The CDs’ intensity can be quantitatively quenched by Hg2+ and Fe3+ ions with high sensitivity and low LOD about 9.58 nM and 22.27 nM, respectively, indicating that the CDs sensors can be potentially applied for Hg2+ and Fe3+ detection in aqueous solutions. Full article
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