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Development and Application of Optical Chemical Sensing

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Optical Sensors".

Deadline for manuscript submissions: 25 January 2027 | Viewed by 2093

Special Issue Editor


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Guest Editor
Laboratory of Analytical Chemistry, Department of Chemistry, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece
Interests: analytical chemistry; sensors; colorimetry; fluorescence; paper-based analytical devices
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Optical chemical sensing is a rapidly evolving field that uses light-based techniques to detect and measure chemical substances. These sensors operate by translating chemical interactions into optical signals—such as changes in fluorescence, color, or light intensity—allowing for fast, instrument-free, non-invasive, and highly sensitive analysis. Over the years, advancements in materials science and photonics have significantly improved sensor performance, enabling miniaturization, lower detection limits, and greater selectivity. Recent developments have focused on enhancing sensor performance through the integration of novel materials, including quantum dots, metal–organic frameworks, and plasmonic nanostructures. These enhancements have broadened the applicability of optical sensors across diverse fields, including analytical chemistry, environmental monitoring, biomedical diagnostics, food analysis, and industrial process control. As the demand for rapid, portable, and low-cost analytical tools grows, optical chemical sensing continues to evolve, offering promising solutions for both laboratory and field-based applications.

Dr. George Tsogkas
Guest Editor

Manuscript Submission Information

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Keywords

  • optical sensing
  • low-cost platforms
  • analytical chemistry
  • paper-based devices
  • pharmaceutical analysis
  • food analysis
  • environmental monitoring
  • smartphone-based analysis
  • nanomaterials
  • point-of-care diagnostics

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Published Papers (2 papers)

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Research

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17 pages, 1176 KB  
Article
Portable Raspberry Pi Platform for Automated Interpretation of Lateral Flow Strip Tests
by Natalia Nakou, Panagiotis K. Tsikas and Despina P. Kalogianni
Sensors 2026, 26(2), 598; https://doi.org/10.3390/s26020598 - 15 Jan 2026
Viewed by 685
Abstract
Paper-based rapid tests are widely used in point-of-care diagnostics due to their simplicity and low cost. However, their application in quantitative analysis remains limited. In this work, a nucleic acid lateral flow assay (NALFA) was integrated with an automated image acquisition system built [...] Read more.
Paper-based rapid tests are widely used in point-of-care diagnostics due to their simplicity and low cost. However, their application in quantitative analysis remains limited. In this work, a nucleic acid lateral flow assay (NALFA) was integrated with an automated image acquisition system built on a Raspberry Pi platform for the quantitative detection of SARS-CoV-2 virus, increasing the accuracy of the test compared to subjective visual interpretation. The assay employed blue polystyrene microspheres as reporters, while automated image capturing, image processing and quantification were performed using custom Python software (version 3.12). Signal quantification was achieved by comparing the grayscale intensity of the test line with that of a simultaneously captured negative control strip, allowing correction for illumination and background variability. Calibration curves were used for the training of the algorithm. The system was applied for the analysis of a series of samples with varying DNA concentrations, yielding recoveries between 84 and 108%. The proposed approach integrates a simple biosensor with an accessible computational platform to achieve full low-cost automation. This work introduces the first Raspberry Pi-driven image processing approach for accurate quantification of NALFAs and establishes a foundation for future low-cost, portable diagnostic systems targeting diverse nucleic acids, proteins, and biomarkers. Full article
(This article belongs to the Special Issue Development and Application of Optical Chemical Sensing)
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Review

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29 pages, 4250 KB  
Review
Paper-Based Analytical Devices Coupled with Fluorescence Detection and Smartphone Imaging: Advances and Applications
by Constantinos K. Zacharis
Sensors 2026, 26(3), 1012; https://doi.org/10.3390/s26031012 - 4 Feb 2026
Viewed by 1015
Abstract
Paper-based analytical devices have emerged as a versatile and cost-effective platform for on-site chemical and biological analysis. The integration of fluorescence detection with smartphone imaging has significantly enhanced the analytical performance and portability of these systems, enabling sensitive, rapid, and user-friendly detection of [...] Read more.
Paper-based analytical devices have emerged as a versatile and cost-effective platform for on-site chemical and biological analysis. The integration of fluorescence detection with smartphone imaging has significantly enhanced the analytical performance and portability of these systems, enabling sensitive, rapid, and user-friendly detection of diverse analytes. This review highlights recent advancements in paper-based fluorescence sensing technologies, focusing on their design principles, materials, and detection strategies. Emphasis is placed on the use of nanomaterials, quantum dots, and carbon-based fluorophores that improve sensitivity and selectivity in food, bioanalytical, and environmental applications. The role of smartphones as optical detectors and data processing tools is explored, underscoring innovations in image analysis, calibration algorithms, and app-based quantification methods. Full article
(This article belongs to the Special Issue Development and Application of Optical Chemical Sensing)
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