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Design, Synthesis and Application of Multi-Functional Fluorescent Probes for Bioimaging

A special issue of Sensors (ISSN 1424-8220). This special issue belongs to the section "Biosensors".

Deadline for manuscript submissions: closed (5 December 2025) | Viewed by 845

Special Issue Editor


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Guest Editor
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
Interests: chemical synthesis; biochemical analysis; medical imaging

Special Issue Information

Dear Colleagues,

Cancers are a major cause of death and create a major burden on public healthcare systems. The pharmacological mechanisms of candidate drugs are central to the translational paradigm. Since many signal transduction and molecular events are implicated in these cancers, a novel method for investigating the key pharmacological mechanisms of candidate drugs is required to accelerate drug discovery. Recently, much attention has been paid to the real-time imaging of molecular events in order to yield new insights into the pathogenesis of diseases. This Special Issue, “Design, Synthesis and Application of Multi-Functional Fluorescent Probes for Bioimaging”, focuses on recent advances in the development of chemical probes for imaging pathological events to facilitate the study of the pharmacodynamics and toxicity involved. Fluorescent imaging is now viewed as an indispensable technique in the field of biological research. Promoting interdisciplinary collaboration is necessary to further refine functional fluorescent probes for diagnostic and therapeutic applications. Researchers from all relevant scientific fields (e.g., biology, chemistry, medicine, environment, engineering, and material sciences) are invited to submit original and review articles that focus on the following:

(1) Fluorescent probes for sensitive biosensing.

(2) Using fluorescent probes to evaluate intracellular signaling cascades.

(3) The pathology and toxicology of molecular events and their implications.

(4) Fluorescent probes for detecting molecular events in pathology and toxicology.

(5) Fluorescent probes related to cell damage.

Prof. Dr. Lijuan Wang
Guest Editor

Manuscript Submission Information

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Keywords

  • fluorescent probes
  • bioimaging
  • pharmacological mechanisms
  • molecular events
  • pathology and toxicology

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Published Papers (1 paper)

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Research

14 pages, 3329 KB  
Article
Simultaneous Down-Regulation of Intracellular hTERT and GPX4 mRNA Using MnO2-Nanosheet Probes to Induce Cancer Cell Death
by Yixin Miao, Tao Zhou, Qinghong Ji and Min Hong
Sensors 2026, 26(3), 836; https://doi.org/10.3390/s26030836 - 27 Jan 2026
Cited by 1 | Viewed by 619
Abstract
Cancer remains a leading global cause of death, with conventional treatments often limited by toxicity and recurrence. Recent advances in gene therapy and nanodrug delivery offer new avenues for precision oncology. Human telomerase reverse transcriptase (hTERT) and glutathione peroxidase 4 (GPX4) are overexpressed [...] Read more.
Cancer remains a leading global cause of death, with conventional treatments often limited by toxicity and recurrence. Recent advances in gene therapy and nanodrug delivery offer new avenues for precision oncology. Human telomerase reverse transcriptase (hTERT) and glutathione peroxidase 4 (GPX4) are overexpressed in many cancers and linked to apoptosis and ferroptosis, respectively. Here, we developed a manganese dioxide nanosheet (MnO2-NS) probe co-loaded with antisense oligonucleotides targeting hTERT and GPX4 mRNA to synergistically down-regulate both genes and induce dual cell death pathways. The probe, assembled via adsorption of fluorescently labeled antisense strands, showed controllable release in the presence of glutathione (GSH). Cellular uptake and antisense release were confirmed in multiple cancer cell lines. The MnO2-NS probe significantly suppressed cell proliferation, outperforming single-target or carrier-only controls. Molecular analyses confirmed reduced hTERT and GPX4 expression, along with GSH depletion, ROS accumulation, and elevated lipid peroxidation—collectively promoting enhanced cancer cell death. In summary, this MnO2-NS-based co-delivery system enables synergistic gene silencing and GSH depletion, enhancing antitumor efficacy and providing a promising strategy for multifunctional nanotherapy. Full article
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