Transient Gene Expression for Rapid Protein and Virus-Vector Supply

Dear Colleagues,

Transient Gene Expression (TGE) is a most important technique for investigating fundamental processes in the life sciences. Google give 34 million hits, when using these three words.

This Special Issue of “Transient Gene Expression for Rapid Protein and Virus-Vector supply” focuses on protein production and on the generation of innovative virus vectors for the widest range of applications in R&D and for potential use in the clinic. Different from stable expression, transient expression allows the delivery of the desired DNA of interest into a culture of animal cells and the protein expression begins literally within minutes when the DNA arrives at the nucleus of cells. Days after such a delivery of DNA, protein can be obtained from such cultures. This allows the study of structure and function of the desired protein, preferable in a purified form, but also frequently just separates from the cells that produced the protein, in sufficient quantities, to execute a large battery of experiments with it, including in vitro or in vivo studies.

We encourage submissions of papers that present various technologies, used with CHO, HEK-293, HeLa and other mammalian or non-mammalian derived cell lines providing access to rapid protein synthesis and to virus vectors. The papers should emphasize readily applicable methods, both for “standard” laboratories, but also for more specialized use at scales of operation that exceed the typical research laboratory (“large scale transient gene expression”). In spite of the fact that virus-mediated transfer of genetic information can be considered transient, such as with the help of Baculovirus vectors, the editors of this Special Issue wish to restrict the issue to submissions of “naked” DNA or RNA as carriers the genes of interests into the host cell system.

Observation, discussion points or concerns that could lead to the use of TGE for clinical manufacture and use of products derived thereof for therapy are encouraged as well.

Prof. Dr. Florian M. Wurm
Dr. Martin Jordan
Guest Editors

Review related to the Special Issue：
Wurm, F.M. CHO Quasispecies—Implications for Manufacturing Processes. Processes 2013, 1, 296-311.
Wurm, F.M.; Wurm, M.J. Cloning of CHO Cells, Productivity and Genetic Stability—A Discussion. Processes 2017, 5, 20.

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