Special Issue "Transient Gene Expression for Rapid Protein and Virus-Vector Supply"

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Biological Systems".

Deadline for manuscript submissions: 30 April 2019

Special Issue Editors

Guest Editor
Prof. Dr. Florian M. Wurm

Swiss Federal Institute of Technology Lausanne, Route Cantonale, 1015 Lausanne, Switzerland
Website | E-Mail
Phone: + 41-79-301-1492
Interests: gene transfer; protein production/manufacturing from cultivated mammalian cells, including regulatory issues, process development; innovative bioreactors for mammalian cells; suspension culture; CHO cells; large scale manufacturing - CHO; orbital shaking; optimizing mammalian cell culture processes; transient gene expression
Guest Editor
Dr. Martin Jordan

Merck Biopharma, Biotech Process Sciences, Corsier-sur-Vevey, Switzerland
Website | E-Mail
Interests: media development; process optimization; transfection; transient expression; cell line generation; glycosylation of recombinant proteins; high throughput culture systems; process intensification

Special Issue Information

Dear Colleagues,

Transient Gene Expression (TGE) is a most important technique for investigating fundamental processes in the life sciences. Google give 34 million hits, when using these three words.

This Special Issue of “Transient Gene Expression for Rapid Protein and Virus-Vector supply” focuses on protein production and on the generation of innovative virus vectors for the widest range of applications in R&D and for potential use in the clinic. Different from stable expression, transient expression allows the delivery of the desired DNA of interest into a culture of animal cells and the protein expression begins literally within minutes when the DNA arrives at the nucleus of cells. Days after such a delivery of DNA, protein can be obtained from such cultures. This allows the study of structure and function of the desired protein, preferable in a purified form, but also frequently just separates from the cells that produced the protein, in sufficient quantities, to execute a large battery of experiments with it, including in vitro or in vivo studies.

We encourage submissions of papers that present various technologies, used with CHO, HEK-293, HeLa and other mammalian or non-mammalian derived cell lines providing access to rapid protein synthesis and to virus vectors. The papers should emphasize readily applicable methods, both for “standard” laboratories, but also for more specialized use at scales of operation that exceed the typical research laboratory (“large scale transient gene expression”). In spite of the fact that virus-mediated transfer of genetic information can be considered transient, such as with the help of Baculovirus vectors, the editors of this Special Issue wish to restrict the issue to submissions of “naked” DNA or RNA as carriers the genes of interests into the host cell system.

Observation, discussion points or concerns that could lead to the use of TGE for clinical manufacture and use of products derived thereof for therapy are encouraged as well.

Prof. Dr. Florian M. Wurm
Dr. Martin Jordan
Guest Editors

Review related to the Special Issue:
Wurm, F.M. CHO Quasispecies—Implications for Manufacturing Processes. Processes 2013, 1, 296-311.
Wurm, F.M.; Wurm, M.J. Cloning of CHO Cells, Productivity and Genetic Stability—A Discussion. Processes 2017, 5, 20.

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1100 CHF (Swiss Francs). Please note that for papers submitted after 30 June 2019 an APC of 1200 CHF applies. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • Transfection, transient gene expression, mammalian cells, insect cells, large-scale, protein production, disposable bioreactors, protein variant analysis, protein structure, protein function, chimaeric proteins, protein design, DNA transfection reagents

Published Papers (1 paper)

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Open AccessArticle Non-Viral Transfection of Human T Lymphocytes
Processes 2018, 6(10), 188; https://doi.org/10.3390/pr6100188
Received: 10 September 2018 / Revised: 27 September 2018 / Accepted: 9 October 2018 / Published: 11 October 2018
PDF Full-text (3255 KB) | HTML Full-text | XML Full-text
The genetic modification of human T lymphocytes with established non-viral methods is inefficient. Linear polyethylenimine (l-PEI), one of the most popular non-viral transfection agents for mammalian cells in general, only achieves transfection rates in the single digit percentage range for these cells. Here, [...] Read more.
The genetic modification of human T lymphocytes with established non-viral methods is inefficient. Linear polyethylenimine (l-PEI), one of the most popular non-viral transfection agents for mammalian cells in general, only achieves transfection rates in the single digit percentage range for these cells. Here, a well-defined 24-armed poly(2-dimethylamino) ethyl methacrylate (PDMAEMA) nanostar (number average of the molecular weight: 755 kDa, polydispersity: <1.21) synthesized via atom transfer radical polymerization (ATRP) from a silsesquioxane initiator core is proposed as alternative. The agent is used to prepare polyplexes with plasmid DNA (pDNA). Under optimal conditions these polyplexes reproducibly transfect >80% of the cells from a human T-cell leukemia cell line (Jurkat cells) at viabilities close to 90%. The agent also promotes pDNA uptake when simply added to a mixture of cells and pDNA. This constitutes a particular promising approach for efficient transient transfection at large scale. Finally, preliminary experiments were carried out with primary T cells from two different donors. Results were again significantly better than for l-PEI, although further research into the response of individual T cells to the transfection agent will be necessary, before either method can be used to routinely transfect primary T lymphocytes. Full article
(This article belongs to the Special Issue Transient Gene Expression for Rapid Protein and Virus-Vector Supply)

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