Special Issue "Biocatalysis, Enzyme and Process Engineering"

A special issue of Processes (ISSN 2227-9717). This special issue belongs to the section "Biological Systems".

Deadline for manuscript submissions: 31 January 2020.

Special Issue Editors

Dr. Pedro Fernandes
E-Mail Website
Guest Editor
1. Department of Bioengineering and IBB, Institute for Bioengineering and Biosciences, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal
2. DREAMS and Faculty of Engineering, Universidade Lusófona de Humanidades e Tecnologias, Lisbon, Portugal
Tel. +351218419065
Dr. Carla C. C. R. de Carvalho
E-Mail Website
Guest Editor
iBB – Institute for Bioengineering and Biosciences, Department of Bioengineering, Instituto Superior Técnico, Universidade de Lisboa, Lisbon, Portugal
Interests: bacterial adaptation, marine biotechnology, biocatalysis, bioreactors, bioprocess engineering
Special Issues and Collections in MDPI journals

Special Issue Information

Dear colleagues,

Enzymes are acknowledged as effective catalysts, namely, on account of their selectivity, high turnover, and ability to operate under mild conditions. Despite these advantageous features, the industrial application of enzymes still lags behind their use on a laboratory scale. This pattern may be partly assigned to the intrinsic nature of enzymes: these biocatalysts have been optimized throughout the ages to be mostly active in aqueous media and to operate in environments where substrate and product concentrations are much lower than those required for industrial processes to be commercially feasible. Hence, high concentrations of substrates or products can be toxic or inhibitory. Moreover, the compounds involved in several of these industrial processes, namely, in the pharmaceutic industry, are often sparingly water-soluble, hence the need to use non-aqueous media, which can pose limitations on several enzymes. Enzyme engineering, often coupled to process engineering, has been shown to provide a sound approach to tackle these issues and improve the catalytic and stability features of several enzymes. In addition, methodologies such as continuous flow, already well-established in chemical processes, have been increasingly used in biocatalysis, given their potential to increase overall bioconversion rates due to increased mass transfer and enhanced throughput of material.

This Special Issue on “Biocatalysis, Enzyme, and Process Engineering” aims to address novel advances in the development and application of enzymes in the production of goods of interest for large-scale production. Topics include but are not limited to the following:

  • Enzyme engineering towards improved biocatalysts;
  • The development of novel process designs for biocatalysis;
  • Multienzyme cascade reactions.

Dr. Pedro Fernandes
Dr. Carla C. C. R. de Carvalho
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Processes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1400 CHF (Swiss Francs). Please note that for papers submitted after 30 June 2020 an APC of 1500 CHF applies. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.


  • biocatalysis
  • bioprocesses
  • cascade reactions
  • continuous flow
  • directed evolution
  • enzyme engineering
  • miniaturization
  • multiphase systems
  • rational design
  • white biotechnology

Published Papers (1 paper)

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Open AccessArticle
Manufacturing Process, In Vivo and In Vitro Digestibility Assessment of an Enteral Feeding Product Hydrolyzed from Locally Available Ingredients Using Commercial Enzymes
Processes 2019, 7(6), 347; https://doi.org/10.3390/pr7060347 - 06 Jun 2019
A ready-for-use enteral feeding product was manufactured based on energy standard mixing using hydrolyzed products from local foods (i.e., loin pork, carrot, pumpkin, soybean, rice, and potato). When compared to enteral foods based on defined ingredients, the manufactured product is more cost-effective, appropriately [...] Read more.
A ready-for-use enteral feeding product was manufactured based on energy standard mixing using hydrolyzed products from local foods (i.e., loin pork, carrot, pumpkin, soybean, rice, and potato). When compared to enteral foods based on defined ingredients, the manufactured product is more cost-effective, appropriately functional and has similar physicochemical properties. Relative protein digestibility for in vitro value was tested by using enzyme pepsin, pH-drop and pH-stat method with three different enzymes. The product was shown to be easily digested with an in vivo digestibility value of 89.7%. Molecule sizes of components in the product ranged from 3.5 to 8.5 kDa, determined by SDS-PAGE, and the average molecular weight was 1.52 kDa, determined by Gel Permeation Chromatography (GPC) method. In terms of nutritional value, the product achieved a caloric density of 1 kcal/mL, dietary fibers of 1.48 g per 100 mL and provided both oligomeric and monomeric forms of protein. In addition, the product has the leucine: isoleucine: valine ratio of 2:1:1, thus facilitating the absorption of the protein. In conclusion, the manufactured enteral feeding product has been shown to be appropriate for providing nutritional support for patients. Full article
(This article belongs to the Special Issue Biocatalysis, Enzyme and Process Engineering)
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