Plant DNA Metabarcoding

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: closed (31 July 2021) | Viewed by 3861

Special Issue Editors


E-Mail Website
Guest Editor
National Institute of Oceanography and Applied Geophysics – OGS, Via Piccard 54, 34151 Trieste, Italy
Interests: DNA metabarcoding; metagenomics; bioinformatics; plant; fungi; microbial biodiversity and ecology

E-Mail Website
Guest Editor
Department of Bioscience and Biotechnology of University of Milano-Bicocca, I-20126 Milano, Italy
Interests: plant biodiversity; agrobiodiversity; molecular identification; food traceability
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

plant detection and identification are fundamental in many types of research including biomonitoring and biodiversity assessment in different environments such as air, soil, and water, diet analysis, invasive and protected species recognition, food and herbal product composition.

Among molecular tools, DNA metabarcoding is becoming the gold standard for these kinds of studies. This technique allows for high taxonomic resolution, reproducibility, and short processing time. However, there is still room for improvement in plant science, from standardized laboratory procedures, to barcode choice, to the availability of curated databases.

This Special Issue of Plants will investigate the broad potential of plant DNA metabarcoding, providing an up-to-date overview of widespread but also novel applications. We encourage papers dealing with the comparison and integration of DNA metabarcoding with other techniques, such as microscopy and qPCR. We also welcome methodological works regarding the evaluation of different molecular and bioinformatic protocols.

Dr. Elisa Banchi
Prof. Massimo Labra
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • plant biodiversity
  • high-throughput sequencing (HTS, NGS)
  • algae
  • pollen
  • herbal products
  • dietary analyses
  • amplicon sequencing

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

14 pages, 2854 KiB  
Article
DNA-Based Herbal Teas’ Authentication: An ITS2 and psbA-trnH Multi-Marker DNA Metabarcoding Approach
by Jessica Frigerio, Giulia Agostinetto, Valerio Mezzasalma, Fabrizio De Mattia, Massimo Labra and Antonia Bruno
Plants 2021, 10(10), 2120; https://doi.org/10.3390/plants10102120 - 6 Oct 2021
Cited by 11 | Viewed by 2965
Abstract
Medicinal plants have been widely used in traditional medicine due to their therapeutic properties. Although they are mostly used as herbal infusion and tincture, employment as ingredients of food supplements is increasing. However, fraud and adulteration are widespread issues. In our study, we [...] Read more.
Medicinal plants have been widely used in traditional medicine due to their therapeutic properties. Although they are mostly used as herbal infusion and tincture, employment as ingredients of food supplements is increasing. However, fraud and adulteration are widespread issues. In our study, we aimed at evaluating DNA metabarcoding as a tool to identify product composition. In order to accomplish this, we analyzed fifteen commercial products with DNA metabarcoding, using two barcode regions: psbA-trnH and ITS2. Results showed that on average, 70% (44–100) of the declared ingredients have been identified. The ITS2 marker appears to identify more species (n = 60) than psbA-trnH (n = 35), with an ingredients’ identification rate of 52% versus 45%, respectively. Some species are identified only by one marker rather than the other. Additionally, in order to evaluate the quantitative ability of high-throughput sequencing (HTS) to compare the plant component to the corresponding assigned sequences, in the laboratory, we created six mock mixtures of plants starting both from biomass and gDNA. Our analysis also supports the application of DNA metabarcoding for a relative quantitative analysis. These results move towards the application of HTS analysis for studying the composition of herbal teas for medicinal plants’ traceability and quality control. Full article
(This article belongs to the Special Issue Plant DNA Metabarcoding)
Show Figures

Figure 1

Back to TopTop