Innovation for In Vitro Plant Propagation and Biotechnological Approaches

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Genetics, Genomics and Biotechnology".

Deadline for manuscript submissions: 31 August 2026 | Viewed by 1987

Special Issue Editor


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Guest Editor
SECIHTI-Postgraduate College-Campus Cordoba, Amatlan de los Reyes, Veracruz 94953, Mexico
Interests: plant health; in vitro conservation of germplasm; breeding; production of secondary metabolites; in vitro plant propagation

Special Issue Information

Dear Colleagues,

Innovation in plant biotechnology refers to the development of new methods and technologies or the modification of a product or process with practical applications. Plant biotechnology, as a scientific discipline, has shown great development in recent years. One key application of plant biotechnology is plant tissue culture (PTC). PTC applications can be classified into five categories: 1) plant health, which consists of obtaining pathogen- free plants by meristem culture, thermotherapy, and cryotherapy; 2) in vitro conservation of germplasm using minimum growth medium and cryopreservation techniques; 3) breeding via genetic transformation, somatic hybridization, mutagenesis, and in vitro selection; 4) production of secondary metabolites with high industrial value; and 5) in vitro plant propagation. Recent developments include the application of nanomaterials, hormesis, the use of bioreactors, and genome editing.

This Special Issue aims to foster a deep understanding of in vitro culture and its applications in plant biotechnology, emphasizing innovation in in vitro plant propagation and biotechnological approaches to address global challenges in food security and biodiversity conservation and contribute to sustainable agricultural practices.

We look forward to receiving your contributions.

Dr. Jericó Jabín Bello Bello
Guest Editor

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Keywords

  • micropropagation
  • nanomaterials
  • bioreactors
  • biostimulation
  • genome editing
 

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Published Papers (2 papers)

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Research

12 pages, 1799 KB  
Article
Evaluation of Different Bioreactors During In Vitro Shoot Proliferation and Acclimatization of Agave (Agave marmorata Roezl)
by María del Rosario Moreno-Hernández, Eucario Mancilla-Álvarez, José Abel López-Buenfil and Jericó Jabín Bello-Bello
Plants 2026, 15(7), 1054; https://doi.org/10.3390/plants15071054 - 30 Mar 2026
Viewed by 823
Abstract
Temporary Immersion Systems (TISs) are an efficient alternative for in vitro plant regeneration. This study aimed to evaluate the effect of different culture methods on the in vitro shoot proliferation and acclimatization of agave (Agave marmorata Roezl). The culture methods compared were [...] Read more.
Temporary Immersion Systems (TISs) are an efficient alternative for in vitro plant regeneration. This study aimed to evaluate the effect of different culture methods on the in vitro shoot proliferation and acclimatization of agave (Agave marmorata Roezl). The culture methods compared were a recipient for automated temporary immersion (RITA®), a temporary immersion bioreactor (TIB), a SETIS™ bioreactor, and a semisolid medium control. After eight weeks of in vitro culture, the hyperhydricity of the explants, development variables, photosynthetic pigment content, stomatal density, and survival percentage during acclimatization were evaluated. The results showed that TISs significantly reduced explant hyperhydricity and increased the multiplication rate, number of shoots and leaves, number of roots per shoot, root length, carotenoid content, stomatal density, and percentage of closed stomata during in vitro shoot proliferation. Furthermore, TISs resulted in a higher number of leaves and roots and improved the survival percentage during acclimatization compared to the semisolid medium. Explants cultured in the SETIS™ bioreactor showed the highest photosynthetic pigment content. In conclusion, the evaluated TISs enhanced the physiological development of the explants, favoring the multiplication rate and survival percentage during the acclimatization of A. marmorata. Full article
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14 pages, 2844 KB  
Article
Influence of the Hybrid Compound La(NO3)3@Zn-MOF on the In Vitro Growth of Sugarcane (Saccharum spp. L.)
by Christian Lisette Muñoz-Ibarra, José Luis Spinoso-Castillo, Daniel Padilla-Chacón, Xóchitl De Jesús García-Zárate, Rodolfo Peña-Rodríguez, María Teresa González-Arnao, Raúl Colorado-Peralta and Carlos Alberto Cruz-Cruz
Plants 2026, 15(4), 609; https://doi.org/10.3390/plants15040609 - 14 Feb 2026
Cited by 1 | Viewed by 618
Abstract
In agriculture, the use of Porous Coordination Polymers (PCPs), also known as Metal–Organic Frameworks (MOFs), has emerged as a promising area of research for biological applications, particularly as long-lasting delivery systems for biostimulant chemical compounds. The objective of this study was to evaluate [...] Read more.
In agriculture, the use of Porous Coordination Polymers (PCPs), also known as Metal–Organic Frameworks (MOFs), has emerged as a promising area of research for biological applications, particularly as long-lasting delivery systems for biostimulant chemical compounds. The objective of this study was to evaluate the effect of different concentrations of the hybrid compound La(NO3)3@Zn-MOF and La(NO3)3·6H2O on the in vitro growth of sugarcane cv. Mex 69–290. To assess the effect on sugarcane (Saccharum spp. L.), plantlets were grown in flasks containing Murashige and Skoog (MS) liquid medium without growth regulators. Each treatment consisted of three independent culture flasks, each containing three sugarcane plantlets, and different concentrations (0, 2.5, 5, 10, and 20 mg L−1) of La(NO3)3@Zn-MOF and La(NO3)3·6H2O were added separately. After 30 days of culture, various growth variables were evaluated, including explant length, number of leaves, number and length of shoots, fresh matter, dry matter, and chlorophyll content (a, b, and total). The 5 mg L−1 concentration of La(NO3)3@Zn-MOF increased the number of shoots and leaves in the sugarcane plantlets, and significant increases in fresh and dry matter were observed, while no statistically significant differences were detected in explant length, shoot length, or chlorophyll a, b, and total chlorophyll. However, inhibitory effects were observed at concentrations of 10 and 20 mg L−1 of La(NO3)3@Zn-MOF and La(NO3)3·6H2O, respectively. In conclusion, the hybrid compound La(NO3)3@Zn-MOF exhibited biostimulatory effects on sugarcane growth and physiology under in vitro conditions, whereas high concentrations of the lanthanum(III) salt caused toxicity symptoms in the sugarcane plantlets. Full article
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