Molecular, Genetic, and Physiological Mechanisms in Trees

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Physiology and Metabolism".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 199

Special Issue Editors


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Guest Editor
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China
Interests: molecular mechanisms of wood formation; gravitropic signal transduction in woody plants; regulation of cambium activity and differentiation; biotechnological improvement of fiber quality and yield

E-Mail Website
Guest Editor Assistant
College of Life Sciences, Northeast Forestry University, Harbin 150040, China
Interests: Biosynthesis of wood cellulose; Assembly and remodeling of the secondary cell wall; Molecular regulation of tension wood formation; Role of microtubules and cytoskeleton in wall deposition

Special Issue Information

Dear Colleagues,

Trees are fundamental components of terrestrial ecosystems, they are central to global carbon cycling, and play a vital role in mitigating climate change. Therefore, understanding the molecular and physiological mechanisms underlying their growth, development, and environmental adaptation is essential if we are to advancing tree biology and ecosystem resilience. In recent years, tree molecular biology has emerged as a core area of innovation in forestry science and technology, and this Special Issue will focus on four key dimensions thereof—advances in tissue culture techniques, the decoding of hormone signaling, the elucidation of stress-resistance mechanisms, and the regulation of physiological metabolism. The aim is to systematically uncover the molecular and physiological basis of tree development and adaptation, thereby providing theoretical insights and technical support for genetic improvement and ecological restoration. We invite submissions to this Special Issue on the “Molecular, Genetic, and Physiological Mechanisms in Trees”, and welcome high-quality original research and review articles from around the globe. Together, we seek to drive paradigm shifts in the field of tree molecular biology.

Topics of interest include, but are not limited to, the following:

  1. Innovations in tissue culture and the optimization of genetic transformation systems;
  2. The spatiotemporal regulation of hormone signaling networks in trees;
  3. The molecular mechanisms of stress resistance and the mining of functional gene resources;
  4. The dynamics and systems regulation of physiological and metabolic processes in trees.

Prof. Dr. Yuxiang Cheng
Guest Editor

Dr. Wenjing Xu
Guest Editor Assistant

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Keywords

  • tree development
  • tissue culture
  • hormone signaling
  • stress resistance
  • physiological regulation

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Published Papers (1 paper)

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Research

17 pages, 7653 KiB  
Article
Generation and Functional Characteristics of CRISPR/Cas9-Edited PtrPHOTs Triple-Gene Mutants in Poplar
by Hongtao Yao, Jiyao Cheng, Yuning Jing, Siran Zhu, Chong Wang and Yuxiang Cheng
Plants 2025, 14(10), 1455; https://doi.org/10.3390/plants14101455 - 13 May 2025
Viewed by 157
Abstract
Phototropins (PHOTs), as blue light receptors, play a pivotal role in plant light signal perception and adaptive regulation, yet their functional characteristics in trees remain poorly understood. In this study, the PHOT gene family was identified in Populus trichocarpa, and it included [...] Read more.
Phototropins (PHOTs), as blue light receptors, play a pivotal role in plant light signal perception and adaptive regulation, yet their functional characteristics in trees remain poorly understood. In this study, the PHOT gene family was identified in Populus trichocarpa, and it included three members, PtrPHOT1, PtrPHOT2.1, and PtrPHOT2.2, all of which were highly expressed in mature leaves. Using CRISPR/Cas9 gene editing technology, triple-gene mutations in the PtrPHOT1/2.1/2.2 (PtrPHOTs) were generated, providing initial insights into the functions of PHOTs in trees. Compared to the wild type (WT), triple-gene ptrphots mutants displayed curved and wrinkled leaves, reduced leaf area, and delayed phototropic responses, indicating the central role of PHOTs in blue light signal perception. The stomatal aperture recovery rate in mutants was only 40% of that observed in WT, accompanied by significant downregulation of the BLUS1 gene transcription levels, confirming the conservation of the PHOT-BLUS1-H⁺-ATPase signaling axis in stomatal regulation. Transcriptome of triple-gene ptrphots mutants revealed 1413 differentially expressed genes, of which were enriched in auxin response (upregulation of SAUR family genes), jasmonic acid (downregulation of JAZ genes), and light signaling pathways, suggesting that PHOTs could regulate plant adaptability by integrating light signals and hormone homeostasis. Overall, this study achieved the knockouts of three PtrPHOTs family genes, and characteristics of triple-gene ptrphots mutants elucidated the multifunctional roles of PHOTs in leaf development, phototropism, and stomatal movement in poplar. Our work provides a foundation for deciphering light signaling networks and molecular breeding in woody plants. Full article
(This article belongs to the Special Issue Molecular, Genetic, and Physiological Mechanisms in Trees)
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