Micropropagation, Acclimatization and Genebanking of Horticultural Crops

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Molecular Biology".

Deadline for manuscript submissions: closed (10 February 2024) | Viewed by 6777

Special Issue Editors


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Guest Editor
Department of Earth and Environment, Florida International University, 11200 SW 8th St, Miami, FL 33199, USA
Interests: in vitro micropropagation; genebanking; cryopreservation; endangered and rare plants; optical sensors; sustainable and conservation horticulture

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Guest Editor
Environmental Horticulture Department, Institute of Food and Agricultural Sciences, University of Florida, 2550 Hull Rd., Gainesville, FL 32611-0670, USA
Interests: micropropagation; cryopreservation; ornamental plants; medicinal plants; horticultural crops; space biology; temporary immersion bioreactors
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Guest Editor
Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia, Serdang 43400, Selangor, Malaysia
Interests: seed science and technology; plant physiology; plant tissue culture; cryopreservation; agronomy; medicinal and aromatic plants; agriculture biotechnology; sustainable agriculture

Special Issue Information

Dear Colleagues,

In vitro propagation (or tissue culture) is an important process in plant propagation. After explant induction and somatic embryo/callus production, plant materials can be placed in a suitable medium to become seedlings or encapsulated and transformed into artificial/synthetic (syn) seeds for short- or medium-term preservation or delivery. Due to seedling mortality during the introduction and reintroduction stages, acclimatization, which is the process of transferring plants from an aseptic artificial environment to the outside world, is a vital final step of in vitro micropropagation. The encapsulation process has advantages over traditional micropropagation that can result in the need for excessive subculturing, unpredictable mutations, larger space requirements, and higher labor needs, which are costly. Synthetic seeds containing propagules (plants’ reproducible organs) such as nodes, shoots, callus, somatic embryos, and protocorms are contained in a gelatinous matrix. The use of axillary buds and shoot tips derived in vitro enables the acquisition of inexpensive, easily obtained propagules for storage. To date, cryopreservation is the only vital methods for conserving plant germplasm and living cells in liquid nitrogen. In the past two decades, cryopreservation has been widely used as an alternative method for plant germplasm storage. We welcome both critical review articles and original research articles focusing chiefly, but not exclusively, on topics related to the following keywords.

Dr. Amir Khoddamzadeh
Prof. Dr. Wagner Vendrame
Dr. Uma Rani Sinniah
Guest Editors

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Keywords

  • in vitro culture
  • micropropagation
  • horticultural crops
  • tissue culture
  • genebanking
  • cryopreser-vation
  • synthetic seed (artificial seed)
  • seed germination
  • bioreactor
  • ornamentals
  • tropical plants
  • subtropical plants
  • acclimatization
  • medicinal plants
  • ornamental plants
  • flowers
  • fruit trees
  • encapsulation
  • embryo rescue
  • plant growth regulators
  • germplasm conservation
  • botanic gardens

Published Papers (1 paper)

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Review

32 pages, 2319 KiB  
Review
Orchid Micropropagation Using Conventional Semi-Solid and Temporary Immersion Systems: A Review
by Potshangbam Nongdam, David G. Beleski, Leimapokpam Tikendra, Abhijit Dey, Vanlalrinchhani Varte, Soumaya EL Merzougui, Vania M. Pereira, Patricia R. Barros and Wagner A. Vendrame
Plants 2023, 12(5), 1136; https://doi.org/10.3390/plants12051136 - 02 Mar 2023
Cited by 4 | Viewed by 5538
Abstract
Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive [...] Read more.
Orchids, with their astonishingly stunning flowers, dominate the international floricultural market. They are considered prized assets for commercial applications in pharmaceutical and floricultural industries as they possess high therapeutic properties and superior ornamental values. The alarming depletion of orchid resources due to excessive unregulated commercial collection and mass habitat destruction makes orchid conservation measures an extreme priority. Conventional propagation methods cannot produce adequate number of orchids, which would meet the requirement of these ornamental plants for commercial and conservational purposes. In vitro orchid propagation using semi-solid media offers an outstanding prospect of rapidly producing quality plants on a large scale. However, the semi-solid (SS) system has shortcomings with low multiplication rates and high production costs. Orchid micropropagation using a temporary immersion system (TIS) overcomes the limitations of the SS system by reducing production costs and making scaleup and full automation possible for mass plant production. The current review highlights different aspects of in vitro orchid propagation using SS and TIS and their benefits and drawbacks on rapid plant generation. Full article
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