Molecular Biology of Ornamental Plants, Volume II

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Horticultural Science and Ornamental Plants".

Deadline for manuscript submissions: closed (31 October 2024) | Viewed by 4901

Special Issue Editors


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Guest Editor
State Key Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
Interests: ornamental plants; bioinformatics; biotechnology; gene editing
Special Issues, Collections and Topics in MDPI journals
College of Agro-Grassland Science, Nanjing Agricultural University, Nanjing 210095, China
Interests: plant molecular biology; protein modification mechanism of plant abiotic stress tolerance; forage and turfgrass breeding
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

As is well known, relative to model plants, ornamental plants have many special characteristics. However, current regulatory models are mainly based on model plants. With the development of sequencing technology and omics tools, we can apply high-throughput sequencing and other omics methods to discover core regulatory networks and genes related to specific ornamental traits. These advances can provide assistance and excellent candidate genes for directional breeding. On the other hand, classical molecular biology methods have also been used to discover the regulatory mechanism of ornamental traits. This will also enrich our understanding of the development mechanism of plant-specific organs. Based on these, we have decided to launch this Special Issue on “Molecular Biology of Ornamental Plants”.

This Special Issue welcomes contributions from researchers working in the field of molecular biology of ornamental plants. Original research articles are encouraged for submission, focusing on but not limited to the following areas:

  • Biotechnology and genome editing;
  • Biotic or abiotic stress-resistant gene function in ornamental plants;
  • Integrative analysis of multi-omics;
  • Molecular regulatory mechanism of ornamental traits.

Dr. Aiping Song
Dr. Yu Chen
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Plants is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

  • biotechnology
  • development
  • molecular regulatory mechanism
  • multi-omics
  • ornamental plants
  • stress response

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Related Special Issue

Published Papers (3 papers)

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Research

17 pages, 7439 KiB  
Article
Somatic Embryogenesis from the Leaf-Derived Calli of In Vitro Shoot-Regenerated Plantlets of Rosa hybrida ‘Carola’
by Mingao Duan, Juan Liu, Yining Zhao, Xiaofei Wang, Longzhen Li, Shiyi Wang, Ruidong Jia, Xin Zhao, Yaping Kou, Kairui Su, Hong Ge and Shuhua Yang
Plants 2024, 13(24), 3553; https://doi.org/10.3390/plants13243553 - 19 Dec 2024
Viewed by 1192
Abstract
Roses are one of the most important flowers applied to landscape, cut flowers, fragrance and food industries widely. As an effective method for plant reproduction, the regeneration via somatic embryos is the most promising method for breed improvement and genetic transformation of woody [...] Read more.
Roses are one of the most important flowers applied to landscape, cut flowers, fragrance and food industries widely. As an effective method for plant reproduction, the regeneration via somatic embryos is the most promising method for breed improvement and genetic transformation of woody plants. However, lower somatic embryogenesis (SE) induction rates and genotypic constraints impede progress in genetic transformation in rose. This study describes a plant regeneration system for the famous red cut flower cultivar Rosa hybrida ‘Carola’. The stems without petioles cultured on Murashige and Skoog (MS) medium supplemented with 1.0 mg·L−1 6-benzylaminopurine (6-BA), 0.05 mg·L−1 a-naphthalene acetic acid (NAA) and 30 g·L−1 sucrose showed the maximum proliferation coefficient of shoots with 3.41 for the micropropagation system. We evaluated the effects of different plant growth regulators (PGRs) on the induction, proliferation and conversion of somatic embryos. The induction rate of calli reached 100% on MS medium supplemented with 2.0 g·L−1 NAA and 30 g·L−1 glucose. The highest induction rate of somatic embryos achieved a frequency of 13.33% on MS medium supplemented with 2.0 mg·L−1 zeatin (ZT), 0.1 mg·L−1 NAA and 30 g·L−1 glucose. The most suitable carbohydrate with 60 g·L−1 glucose resulted in a proliferation rate of somatic embryos (4.02) on MS medium containing 1.5 mg·L−1 ZT, 0.2 mg·L−1 NAA and 0.1 mg·L−1 gibberellic acid (GA3). The highest somatic embryos germination rate (43.33%) was obtained from the MS medium supplemented with 1.0 mg·L−1 6-BA, 0.01 mg·L−1 IBA and 30 g·L−1 glucose. Finally, the germinated somatic embryos successfully rooted on 1/2 MS medium containing 1.0 mg·L−1 NAA, 30 g·L−1 sucrose, and the vigorous plantlets were obtained after hardening-off culture. This study provided a stable and efficient protocol for plant regeneration via somatic embryos in R. hybrida ‘Carola’, which will be beneficial to the further theoretical study and genetic improvement in roses. Full article
(This article belongs to the Special Issue Molecular Biology of Ornamental Plants, Volume II)
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12 pages, 4544 KiB  
Article
Genetic Analysis and Construction of a Fingerprint for Licensed Triadica sebifera Cultivars Using SSR Markers
by Qi Zhou, Baiqiang Chen, Dongyue Jiang, Fei Zhuge and Yingang Li
Plants 2024, 13(13), 1767; https://doi.org/10.3390/plants13131767 - 26 Jun 2024
Cited by 3 | Viewed by 1665
Abstract
Triadica sebifera is an important landscaping tree species because of its colorful autumn leaves. In recent years, some cultivars have been bred and licensed, but it can be difficult to identify them from their morphological traits due to their similar phenotypes. To explore [...] Read more.
Triadica sebifera is an important landscaping tree species because of its colorful autumn leaves. In recent years, some cultivars have been bred and licensed, but it can be difficult to identify them from their morphological traits due to their similar phenotypes. To explore the genetic relationships and construct a fingerprint of the cultivars, the licensed T. sebifera cultivars were analyzed using SSR markers. A total of 179 alleles were identified among the 21 cultivars at 16 SSR loci, and these alleles exhibited a high level of genetic diversity (He = 0.86). The genetic variations mainly occurred among cultivars based on an analysis of molecular variance (AMOVA). According to phylogenetic analysis, principal coordinate analysis (PCoA), and Bayesian clustering analysis, the genetic relationships were independent of geographic distances, which may be mainly due to transplantations between regions. Some cultivars with different leaf colors showed obvious genetic differentiation and may be preliminary candidates for cross-breeding. Finally, the fingerprint for the licensed cultivars was constructed with two SSR markers. The results of this study can provide technical support for the application and legal protection of licensed Triadica sebifera cultivars. Full article
(This article belongs to the Special Issue Molecular Biology of Ornamental Plants, Volume II)
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15 pages, 7251 KiB  
Article
Characterisation and Expression Analysis of LdSERK1, a Somatic Embryogenesis Gene in Lilium davidii var. unicolor
by Shaojuan Wang, Xiaoyan Yi, Lijuan Zhang, Muhammad Moaaz Ali, Mingli Ke, Yuxian Lu, Yiping Zheng, Xuanmei Cai, Shaozhong Fang, Jian Wu, Zhimin Lin and Faxing Chen
Plants 2024, 13(11), 1495; https://doi.org/10.3390/plants13111495 - 29 May 2024
Cited by 3 | Viewed by 1592
Abstract
The Lanzhou lily (Lilium davidii var. unicolor) is a variant of the Sichuan lily of the lily family and is a unique Chinese ‘medicinal and food’ sweet lily. Somatic cell embryogenesis of Lilium has played an important role in providing technical [...] Read more.
The Lanzhou lily (Lilium davidii var. unicolor) is a variant of the Sichuan lily of the lily family and is a unique Chinese ‘medicinal and food’ sweet lily. Somatic cell embryogenesis of Lilium has played an important role in providing technical support for germplasm conservation, bulb propagation and improvement of genetic traits. Somatic embryogenesis receptor-like kinases (SERKs) are widely distributed in plants and have been shown to play multiple roles in plant life, including growth and development, somatic embryogenesis and hormone induction. Integrating the results of KEGG enrichment, GO annotation and gene expression analysis, a lily LdSERK1 gene was cloned. The full-length open reading frame of LdSERK1 was 1875 bp, encoding 624 amino acids. The results of the phylogenetic tree analysis showed that LdSERK1 was highly similar to rice, maize and other plant SERKs. The results of the subcellular localisation in the onion epidermis suggested that the LdSERK1 protein was localised at the cell membrane. Secondly, we established the virus-induced gene-silencing (VIGS) system in lily scales, and the results of LdSERK1 silencing by Tobacco rattle virus (TRV) showed that, with the down-regulation of LdSERK1 expression, the occurrence of somatic embryogenesis and callus tissue induction in scales was significantly reduced. Finally, molecular assays from overexpression of the LdSERK1 gene in Arabidopsis showed that LdSERK1 expression was significantly enhanced in the three transgenic lines compared to the wild type, and that the probability of inducing callus tissue in seed was significantly higher than that of the wild type at a concentration of 2 mg/L 2,4-D, which was manifested by an increase in the granularity of the callus tissue. Full article
(This article belongs to the Special Issue Molecular Biology of Ornamental Plants, Volume II)
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