Machine Learning for Plant Phenotyping in Wheat

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Modeling".

Deadline for manuscript submissions: 31 July 2026 | Viewed by 509

Special Issue Editors

Regional Technique Innovation Center for Wheat Production, Key Laboratory of Crop Physiology and Ecology in Southern China, Ministry of Agriculture, Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry, Jiangsu Key Laboratory for Information Agriculture, Nanjing Agricultural University, Nanjing 210095, China
Interests: wheat; abiotic stress; crop production; plant phenotyping; deep learning

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Guest Editor
Shandong Engineering Research Center of Agricultural Equipment Intelligentization, Shandong Key Laboratory of Intelligent Production Technology and Equipment for Facility Horticulture, College of Mechanical and Electronic Engineering, Shandong Agricultural University, Tai’an 271018, China
Interests: intelligent control technology for agricultural equipment; research on key technologies of robots; intelligent detection technology

Special Issue Information

Dear Colleagues,

In recent years, the integration of automation control and optical sensing technologies has led to the development of various high-throughput, non-destructive phenotyping platforms, including handheld, gantry, and drone-based systems for both indoor and outdoor applications. These advancements have established a multi-scale, comprehensive plant temporal phenotyping framework, spanning from above- to below-ground, from morphological to physiological, and from macroscopic to microscopic levels. These significantly improve data collection efficiency and precision, while also causing an explosion in phenotypic data.

As one of the most critical food crops, wheat’s precise phenotyping is crucial for genetic improvement and high-yield cultivation. Researchers have amassed vast wheat 2D images (visible light, multispectral/hyperspectral, thermal infrared, and chlorophyll fluorescence) and 3D point cloud data, which urgently require in-depth exploration. Machine learning (ML) and its subfield, deep learning (DL), have played pivotal roles in processing complex data, thereby providing powerful tools for efficient, accurate, and automated analysis of large-scale phenotypic datasets. Therefore, this Special Issue aims to explore and showcase the latest research progress, methodologies, and applications of ML in wheat phenotyping studies, focusing on advancements that improving wheat genetic breeding and high-efficiency production management. Topics include, but are not limited to, the application of ML and DL techniques in the following:

  • Micro-scale phenotypic trait analysis: e.g., morphology of stomata, starch granules, and other physiological micro-indicators.
  • Organ-scale phenotypic trait analysis: e.g., seed, root, stem, leaf, and spike.
  • Individual-scale phenotypic trait analysis: e.g., plant architecture and phenological stages.
  • Population-scale phenotypic trait analysis: e.g., uniformity and yield.
  • Applications in analyzing other critical 2D and 3D wheat phenotypic traits.
  • Development of predictive/estimation models for key wheat phenotypic traits.
  • Genotype–phenotype associations: Integration of ML with genomic data to predict phenotypic outcomes, thereby supporting marker-assisted breeding and genomic selection.

We look forward to receiving your contributions.

Dr. Qing Li
Prof. Dr. Ping Liu
Guest Editors

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Keywords

  • machine learning
  • deep learning
  • multi-scale phenotype
  • multi-data analysis
  • modeling

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Published Papers (1 paper)

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Research

22 pages, 8042 KB  
Article
WSF: A Transformer-Based Framework for Microphenotyping and Genetic Analyzing of Wheat Stomatal Traits
by Honghao Zhou, Haijiang Min, Shaowei Liang, Bingxi Qin, Qi Sun, Zijun Pei, Qiuxiao Pan, Xiao Wang, Jian Cai, Qin Zhou, Yingxin Zhong, Mei Huang, Dong Jiang, Jiawei Chen and Qing Li
Plants 2025, 14(19), 3016; https://doi.org/10.3390/plants14193016 - 29 Sep 2025
Viewed by 391
Abstract
Stomata on the leaves of wheat serve as important gateways for gas exchange with the external environment. Their morphological characteristics, such as size and density, are closely related to physiological processes like photosynthesis and transpiration. However, due to the limitations of existing analysis [...] Read more.
Stomata on the leaves of wheat serve as important gateways for gas exchange with the external environment. Their morphological characteristics, such as size and density, are closely related to physiological processes like photosynthesis and transpiration. However, due to the limitations of existing analysis methods, the efficiency of analyzing and mining stomatal phenotypes and their associated genes still requires improvement. To enhance the accuracy and efficiency of stomatal phenotype traits analysis and to uncover the related key genes, this study selected 210 wheat varieties. A novel semantic segmentation model based on transformer for wheat stomata, called Wheat Stoma Former (WSF), was proposed. This model enables fully automated and highly efficient stomatal mask extraction and accurately analyzes phenotypic traits such as the length, width, area, and number of stomata on both the adaxial (Ad) and abaxial (Ab) surfaces of wheat leaves based on the mask images. The model evaluation results indicate that coefficients of determination (R2) between the predicted values and the actual measurements for stomatal length, width, area, and number were 0.88, 0.86, 0.81, and 0.93, respectively, demonstrating the model’s high precision and effectiveness in stomatal phenotypic trait analysis. The phenotypic data were combined with sequencing data from the wheat 660 K SNP chip and subjected to a genome-wide association study (GWAS) to analyze the genetic basis of stomatal traits, including length, width, and number, on both adaxial and abaxial surfaces. A total of 36 SNP peak loci significantly associated with stomatal traits were identified. Through candidate gene identification and functional analysis, two genes—TraesCS2B02G178000 (on chromosome 2B, related to stomatal number on the abaxial surface) and TraesCS6A02G290600 (on chromosome 6A, related to stomatal length on the adaxial surface)—were found to be associated with stomatal traits involved in regulating stomatal movement and closure, respectively. In conclusion, our WSF model demonstrates valuable advances in accurate and efficient stomatal phenotyping for locating genes related to stomatal traits in wheat and provides breeders with accurate phenotypic data for the selection and breeding of water-efficient wheat varieties. Full article
(This article belongs to the Special Issue Machine Learning for Plant Phenotyping in Wheat)
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