Microbial Formulations to Increase Crop Production, from Laboratory to Fields (2nd Edition)

A special issue of Plants (ISSN 2223-7747). This special issue belongs to the section "Plant Protection and Biotic Interactions".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 3582

Special Issue Editor


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Guest Editor
Ecology and Survival of Microorganisms Group, Laboratorio de Ecología Molecular Microbiana, Centro de Investigaciones en Ciencias Microbiológicas, Instituto de Ciencias, Benemérita Universidad Autónoma de Puebla, Edificio IC11, Ciudad Universitaria, Puebla, Mexico
Interests: microbiology; biotechnology; plant biotechnology; plant physiology; molecular cloning; antimicrobials
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Special Issue Information

Dear Colleagues,

Today crop production is based on technologies related to green revolution, like application of nitrogen fertilizers, pesticides, herbicides, among other strategies. However, all the world is suffering consequences of applications of those products, like problems in health, climatic change, environmental pollution. Therefore, the use of more eco-friendly strategies to maintain or increase the crop production is desirable. Microorganisms could be a good alternative, because several of them are able to interact with plants making a symbiosis or an excellent association giving beneficial characteristics to plants. Mechanisms that bacteria offer to plants involve nitrogen fixation, phosphorous solubilization, hormone production, ACC deaminase activity, inhibition of pathogenic strains, triggering of ISR response, control of pest, protection to plant stress, among others. Research about microorganisms with beneficial potential to plants has been performed in different levels, since the isolation of new microorganisms with potential as a plant growth promoting microorganisms (PGPB), until their use in fields and commercialization. Therefore, this special will try to join frontier research in all levels of the development of a microbial formulation that could be applied on crops with the end to substitute technologies affecting the environment and health of habitants of the earth planet.

This special include but is not limited to next scopes:

1) Isolation and characterization of new beneficial microorganisms to plants

2) Microbiomes of plants as future prospects to consider on the inoculation of the correct biome on plants.

3) Mechanisms of microorganisms involved in the plant growth promotion or other beneficial aspects to plants.

4) Experiments of inoculation in the level of greenhouse.

5) Microbial formulations and its action in fields.

6) Patents related to the application of microorganisms on plant crop production.

7) Commercialization of microbial formulations, experiences and reality respect to products related to green revolution.

Dr. Jesus Munoz Rojas
Guest Editor

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Keywords

  • plant growth promoting microorganisms
  • microbial formulations
  • plant-microbe interactions
  • plant biotechnology

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Published Papers (2 papers)

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Research

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15 pages, 1464 KiB  
Article
The Application of a Foliar Spray Containing Methylobacterium symbioticum Had a Limited Effect on Crop Yield and Nitrogen Recovery in Field and Pot-Grown Maize
by Manuel Ângelo Rodrigues, Carlos Manuel Correia and Margarida Arrobas
Plants 2024, 13(20), 2909; https://doi.org/10.3390/plants13202909 - 17 Oct 2024
Cited by 3 | Viewed by 1529
Abstract
In this study, the effectiveness of an inoculant containing a nitrogen (N)-fixing microorganism (Methylobacterium symbioticum) was evaluated on maize (Zea mays L.) grown both in the field (silage maize) and in pots over two years (2021 and 2022). The field [...] Read more.
In this study, the effectiveness of an inoculant containing a nitrogen (N)-fixing microorganism (Methylobacterium symbioticum) was evaluated on maize (Zea mays L.) grown both in the field (silage maize) and in pots over two years (2021 and 2022). The field trial included the following two treatments: with (Yes) and without (No) the inoculant. The pot experiment was designed as a factorial arrangement with two factors: the application of the inoculant (Yes and No) and N applied to the soil (0, 0.4, 0.8, and 1.6 g pot−1). In the field, total dry matter yield (DMY) did not differ significantly between treatments, although the average DMY was higher in the inoculant treatment. In pots, the total DMY varied significantly across all N rates but was only significantly affected by the inoculant application in 2022. N fixation estimates in the field were 58.8 and 14.5 kg ha−1 for 2021 and 2022, respectively, representing 23.7% and 9.1% of the N recovered in the aboveground plant parts. In pots, the estimated fixed N values were −49.2 and 199.2 mg pot−1 in 2021 and 2022, respectively, which corresponded to −5.2% and 18.5% of the N found in the aboveground plant parts. Considering the average values obtained across the four cultivation conditions, there was a positive outcome for the treated plants. However, these values cannot be considered significant when compared to nitrogen removal in maize crops. A commercial product should provide an unequivocal and quantitatively relevant contribution to plant nutrition, which did not appear to be the case. Thus, for this inoculant to provide reliable guarantees of positive outcomes for farmers and become a useful tool in promoting more sustainable agriculture, further studies appear necessary. These studies should aim to determine in which crops and under what cultivation conditions the application of the inoculant is truly effective in enhancing N fixation and improving crop productivity. Full article
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Review

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15 pages, 3246 KiB  
Review
Cross-Kingdom RNA Transport Based on Extracellular Vesicles Provides Innovative Tools for Plant Protection
by Yujin Zhao, Yanguang Zhou, Jingyan Xu, Sen Fan, Na Zhu, Qingling Meng, Shijie Dai and Xiaofeng Yuan
Plants 2024, 13(19), 2712; https://doi.org/10.3390/plants13192712 - 27 Sep 2024
Cited by 1 | Viewed by 1537
Abstract
RNA interference (RNAi) shows great potential in plant defense against pathogens through RNA-mediated sequence-specific gene silencing. Among RNAi-based plant protection strategies, spray-induced gene silencing (SIGS) is considered a more promising approach because it utilizes the transfer of exogenous RNA between plants and microbes [...] Read more.
RNA interference (RNAi) shows great potential in plant defense against pathogens through RNA-mediated sequence-specific gene silencing. Among RNAi-based plant protection strategies, spray-induced gene silencing (SIGS) is considered a more promising approach because it utilizes the transfer of exogenous RNA between plants and microbes to silence target pathogen genes. The application of nanovesicles significantly enhances RNA stability and delivery efficiency, thereby improving the effectiveness of SIGS and further enhancing plant resistance to diseases and pathogens. This review explores the role of RNAi in plant protection, focusing on the cross-kingdom transport of small RNAs (sRNAs) via extracellular vesicles. It also explores the potential of nanotechnology to further optimize RNA-based plant protection, offering innovative tools and methods in modern plant biotechnology. Full article
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