Advances in Mosquito Insecticide Resistance, Vector Competence and Control Strategies

A special issue of Pathogens (ISSN 2076-0817).

Deadline for manuscript submissions: 25 November 2026 | Viewed by 810

Editors


E-Mail Website1 Website2
Guest Editor
Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Arboviral Diseases Branch, Entomology and Ecology Team, Fort Collins, CO 80521, USA
Interests: insecticide resistance; mosquitoes; vector control; medical entomology; arboviruses
Special Issues, Collections and Topics in MDPI journals

E-Mail Website1 Website2
Guest Editor
Centers for Disease Control and Prevention, Division of Vector-Borne Diseases, Arboviral Diseases Branch, Virology Team, Fort Collins, CO 80521, USA
Interests: arboviruses; virus-vector interactions; virus evolution; medical entomology; metagenomics; pathogen detection
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Arboviral pathogens such as Oropouche, chikungunya, dengue, and West Nile viruses continue to emerge and expand, contributing substantially to global human morbidity and mortality. Despite ongoing efforts, effective vector control remains a persistent challenge. Insecticides play an essential role in vector control; however, gaps remain in our understanding of the extent, distribution, intensity, and management of insecticide resistance.

While research on insecticide resistance in mosquitoes has increased over time, there remain regions where chemical control is routinely employed due to a lack of thorough insecticide resistance characterization. An additional and important unresolved question is whether insecticide-driven selective pressures may alter vector competence, thereby potentially influencing transmission dynamics. It is also important to understand how insecticide resistance influences operational control strategies and efficacy in field scenarios.

This Special Issue strives to address these challenges by highlighting innovative research that advances our understanding of insecticide resistance and vector competence and control. We particularly encourage studies that examine the intersections of these areas, including how insecticide resistance may influence pathogen transmission or the effectiveness of control programs. While original research articles are preferred, well-focused reviews that clarify current gaps and future research needs are also welcome.

Our goal is to link these topics to their implications on human health outcomes with the ultimate objective of improving public health through vector control.

We welcome submissions addressing the following:

  • Current strategies for insecticide rotation and their operational outcomes;
  • Efficacy and evaluation of novel insecticides;
  • Vector competence of insecticide-resistant mosquito populations;
  • Novel control strategies;
  • Documentation of insecticide resistance in understudied vectors;
  • Field trials and case studies demonstrating real-world implementation and impact.

Dr. Casey Parker-Crockett
Dr. Joan L. Kenney
Guest Editors

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Keywords

  • insecticide resistance
  • vector competence
  • operational vector control
  • novel interventions
  • medical entomology
  • mosquitoes

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Published Papers (1 paper)

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8 pages, 389 KB  
Brief Report
Effect of Cup Material on S-Methoprene Bioavailability and Susceptibility Estimates in Larval Mosquito Bioassays
by Sarah S. Wheeler, Kara Kelley and Mario Novelo
Pathogens 2026, 15(7), 769; https://doi.org/10.3390/pathogens15070769 - 22 Jul 2026
Viewed by 339
Abstract
Mosquito control programs rely on larvicides such as S-methoprene to suppress vector populations and prevent mosquito-borne diseases including West Nile virus (WNV). Resistance surveillance is essential for maintaining the long-term effectiveness of larvicide active ingredients and is typically conducted using cup bioassays to [...] Read more.
Mosquito control programs rely on larvicides such as S-methoprene to suppress vector populations and prevent mosquito-borne diseases including West Nile virus (WNV). Resistance surveillance is essential for maintaining the long-term effectiveness of larvicide active ingredients and is typically conducted using cup bioassays to evaluate larval susceptibility. Disposable Styrofoam cups are commonly used for these assays because they minimize adsorption of S-methoprene under standard testing conditions. However, recent restrictions on expanded polystyrene products in California necessitated the identification and evaluation of suitable replacement cup materials for larval resistance bioassays. Four alternative cup materials, high-density polyethylene (HDPE), polyethylene terephthalate (PET), polypropylene (PP), and paper cups, were compared to Styrofoam cups. Cup bioassays were conducted using a laboratory colony of Culex quinquefasciatus exposed to S-methoprene concentrations of 1–40 ppb. Emergence inhibition data were analyzed using probit regression to estimate the concentrations causing 50% and 90% emergence inhibition (LC50 and LC90, respectively) for each cup type. Cup material significantly affected concentration-response relationships. HDPE and paper cups produced significantly higher LC50 and LC90 estimates than Styrofoam, whereas PET and PP produced responses more comparable to the Styrofoam reference. Concentration-response slopes were similar among cup materials, indicating that cup material primarily shifted the concentration required to achieve a given level of emergence inhibition. These findings demonstrate that cup material can significantly influence estimated S-methoprene LC values. Replacement assay cups should therefore be validated before implementation in larval resistance monitoring programs. Full article
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