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Special Issue "Selected Papers from the 8th International Conference on Biotechnology and Bioengineering (8-ICBB 2018)"

A special issue of Molecules (ISSN 1420-3049).

Deadline for manuscript submissions: 31 October 2019

Special Issue Editor

Guest Editor
Dr. Antonio Trincone

Istituto di Chimica Biomolecolare, Consiglio Nazionale delle Ricerche, Comprensorio Olivetti, Edificio 70, Via Campi Flegrei 34, I-80078 Pozzuoli, Napoli, Italy
Website | E-Mail
Fax: +39 081 8041770
Interests: biocatalysis; marine enzymes; marine glycosidases; marine biotechnology; oligosaccharides

Special Issue Information

Dear Colleagues,

The 2018 International Conference on Biotechnology and Bioengineering (ICBB 2018) will be held in Budapest, Hungary, 24–26 October, 2018. The conference is co-organized by the Asia-Pacific Association of Science, Engineering and Technology (APASET), the Offenburg University of Applied Sciences, the Szent Istvan University, the Chinese Journal of Biologicals, the Institute of Bioorganic Chemistry–Polish Academy of Sciences and the University of Catania.The conference has been designed to provide an innovative and comprehensive overview of biotechnology and bioengineering, and a focus will be given to major research advances, including New Approaches and Methods in Biotechnology; Cell and Immunology; Genomics and Molecular Biology; Biomaterials, Biopolymers and Bioenergy; Biomedicine, Pharmacology and Toxicology; Biomedical Engineering and Techniques, and other related aspects.More information about the conference can be found at http://icbb.apaset.org/Participants of the conference are cordially invited to contribute original research papers or reviews to this Special Issue of Molecules. This Special Issue welcomes submission of previously unpublished manuscripts from original work on all aspects of molecular biology, molecular medicine, natural product chemistry, etc.

Dr. Antonio Trincone
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Published Papers (1 paper)

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Research

Open AccessArticle
Sampling for DUS Test of Flower Colors of Ranunculus asiaticus L. in View of Spatial and Temporal Changes of Flower Colorations, Anthocyanin Contents, and Gene Expression Levels
Molecules 2019, 24(3), 615; https://doi.org/10.3390/molecules24030615
Received: 21 January 2019 / Revised: 5 February 2019 / Accepted: 7 February 2019 / Published: 10 February 2019
PDF Full-text (5274 KB) | HTML Full-text | XML Full-text
Abstract
Sampling for DUS test of flower colors should be fixed at the stages and sites that petals are fully colored, and besides, flower colorations are uniform among individuals and stable for a period of time to allow testers to get consistent results. It [...] Read more.
Sampling for DUS test of flower colors should be fixed at the stages and sites that petals are fully colored, and besides, flower colorations are uniform among individuals and stable for a period of time to allow testers to get consistent results. It remains a problem since spatial and temporal flower colorations are reported a lot but their change traits are little discussed. In this study, expression state, uniformity and stability of color phenotypes, anthocyanin contents, and gene expression levels were taken into account based on measurements at 12 development stages and three layers (inner, middle, and outer petals) of two varieties of Ranunculus asiaticus L. to get their best sampling. Our results showed that, outer petals of L9–L10 (stage 9–stage 10 of variety ‘Jiaoyan zhuanhong’) and C5–C6 (stage 5–stage 6 of variety ‘Jiaoyan yanghong’) were the best sampling, respectively. For DUS test, it is suggested to track flower colorations continuously to get the best sampling as well as representative colors since different cultivars had different change traits, and moreover, full expression of color phenotypes came later and lasted for a shorter duration than those of anthocyanin contents and gene expressions. Our innovation exists in following two points. Firstly, a model of change dynamic was introduced to illustrate the change traits of flower colorations, anthocyanin contents, and gene expressions. Secondly, genes used for expression analysis were screened on account of tentative anthocyanins, which were identified based on comparison between liquid chromatography–mass spectrometry (LC–MS) results and molecular mass and mass fragment pattern (M2) of each putative anthocyanin and their fragments deduced in our previous study. Gene screening in this regard may also be interest for other non-model plant genera with little molecular background. Full article
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