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Antioxidant, Anti-Inflammatory Activities and Skin Protective Potential of Extractions from Natural Plants

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: 28 February 2026 | Viewed by 1759

Special Issue Editors


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Guest Editor
Natural Products Laboratory (LPN), Postgraduate Program in Chemistry (PPGQ), Federal University of Piaui (UFPI), Teresina 64049-550, PI, Brazil
Interests: natural products; antioxidant activity; phenolic compounds; toad venom; dereplication; metabolomics

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Guest Editor
Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina 64049-550, Brazil
Interests: chemoprevention; cancer biology; animal models; biochemistry of inflammation; cell signalling
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The use of medicinal plants as agents to prevent or cure a lot of diseases depends on the presence of compounds with pharmacological activities, which can be considered in the development of herbal medicines, active ingredients for medicines, nutraceuticals, and sunscreens, among others.

Since inflammation is recognized as a risk factor or starting point for premature aging and several diseases, such as obesity, atherosclerosis, Alzheimer, Parkinson, cancers, osteoporosis, acute myocardial infarction, asthma, cerebrovascular diseases, diabetes mellitus, and psoriasis, there has been a revived interest in natural products from pharmaceutical industries because they have properties that make them more favorable as leading structures for drug discovery as antioxidant, anti-inflammatory, and skin protective options. Moreover, the overuse or long-term consumption of anti-inflammatory molecules has been associated with toxicity and side effects, including osteoporosis, immunosuppression, atrophy muscle, dyslipidemia, hypertension, peripheral insulin resistance, hyperglycemia, growth retardation and asthma in children, decreased libido, impotence, glaucoma, cataracts, sleep disorders, irritability, depression (with steroidal medications), as well as gastric irritation, peptic ulcer, acute hepatitis, and bleeding predominantly with NSAIDs.

This Special Issue will focus on the latest advances and discoveries of compounds and/or extracts characterized with antioxidant, anti-inflammatory, and skin protective potential from natural plants, as well as new techniques to combat oxidative and anti-inflammatory processes and their applications. Topics include, but are not limited to, antioxidant and anti-inflammatory activities, skin protective potential, and new strategies to evaluate these biological activities (in silico, in vitro, and/or in vivo). We look forward to receiving your contributions and promoting meaningful discussions in this important field.

Dr. Gerardo Magela Vieira-Júnior
Dr. Paulo Michel Pinheiro Ferreira
Guest Editors

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Keywords

  • antioxidants
  • anti-inflammatory
  • skin protection
  • phenolic compounds
  • extracts derived from plants
  • natural products

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Published Papers (3 papers)

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Research

17 pages, 3100 KB  
Article
Premna puberula P. Ethyl Acetate Extract Treats Ulcerative Colitis by Regulating the Intestinal Flora and Improving Serum Metabolism
by Zhichao Wang, Yanmei Zhang, Yun Huang, Qiang Xiao, Yuchang Zhu and Dazhai Zhou
Molecules 2025, 30(18), 3809; https://doi.org/10.3390/molecules30183809 - 19 Sep 2025
Viewed by 240
Abstract
The prevalence of Ulcerative Colitis (UC) is continuously increasing globally, demanding the urgent search for new treatment agents due to the limitations of existing therapies. Premna puberula Pamp. (PP), a traditional medicinal and dietary plant, has anti-inflammatory properties. Its extracts’ therapeutic benefits for [...] Read more.
The prevalence of Ulcerative Colitis (UC) is continuously increasing globally, demanding the urgent search for new treatment agents due to the limitations of existing therapies. Premna puberula Pamp. (PP), a traditional medicinal and dietary plant, has anti-inflammatory properties. Its extracts’ therapeutic benefits for UC have not been documented, though. Therefore, we aim to investigate the therapeutic effects of PPEAC on UC, providing a reference for new UC therapies. In our study, we used UPLC-MS/MS to determine the composition of Premna puberula Pamp’s ethyl acetate extract (PPEAC). We assessed the effectiveness of PPEAC using a UC mouse model. The results demonstrated that PPEAC significantly reduced the Disease Activity Index (DAI) scores, lowered liver and spleen weight ratios, mitigated colonic shortening and histopathological damage, and alleviated oxidative stress. This research represented the first systematic investigation into the molecular mechanism of PPEAC ameliorating UC by modulating the intestinal flora-metabolism axis. PPEAC appeared to have a therapeutic impact on UC by boosting phosphatidylcholine (PC) analog levels and the number of Firmicutes and Muribaculaceae, as well as altering alpha-linolenic acid metabolism. Our research provided a new therapeutic approach for using PP as a traditional functional plant for food and medicine, as well as a new viewpoint for the creation of UC-targeted treatments based on conventional herbs. Full article
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16 pages, 7287 KB  
Article
Gardeniae Fructus Enhances Skin Barrier Function via AHR-Mediated FLG/LOR/IVL Expression
by Kaile Zong, Fangni Zhou, Kewei Xu, Junzi Dong, Qing Huang and Jianxin Wu
Molecules 2025, 30(18), 3764; https://doi.org/10.3390/molecules30183764 - 16 Sep 2025
Viewed by 303
Abstract
Gardeniae Fructus (GF), a traditional Chinese medicine rich in iridoids, has demonstrated skin-improving effects. However, its mechanisms for enhancing epidermal barrier function remain unclear. In this study, the iridoids in GF were characterized using UPLC-MS/MS. The improvement in the barrier function by GF [...] Read more.
Gardeniae Fructus (GF), a traditional Chinese medicine rich in iridoids, has demonstrated skin-improving effects. However, its mechanisms for enhancing epidermal barrier function remain unclear. In this study, the iridoids in GF were characterized using UPLC-MS/MS. The improvement in the barrier function by GF was assessed through in vitro experiments and a human efficacy assessment. In addition, the potential targets were predicted through proteomics analysis, molecular docking, and molecular dynamics (MD), and verified in HaCaT cells and three-dimensional epidermal models. Nine iridoids were identified in GF. In vitro, GF effectively promoted cell migration and reduced cell damage and oxidative stress. Proteomics analysis combined with molecular docking and MD simulations predicted that the primary iridoids in GF ameliorate barrier function by binding to the aryl hydrocarbon receptor (AHR) with high affinity and stability. Subsequent validation demonstrated that GF significantly upregulated AHR, filaggrin (FLG), loricrin (LOR), and involucrin (IVL) mRNA and protein expression. A 28-day randomized double-blind human efficacy assessment in subjects with sensitive skin showed that the gel with GF increased stratum corneum hydration, reduced transepidermal water loss (TEWL), and lowered erythema index and lactic acid tingling. These findings suggest that GF enhances the skin barrier via AHR activation-mediated upregulation of barrier proteins, supporting its cosmeceutical potential. Full article
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25 pages, 2652 KB  
Article
Establishment of Callus Cultures from Dalbergia sissoo Leaf Explants for Production of Skin Therapeutics: An In Vitro and In Silico Study
by Jyotsana and Promila Gupta
Molecules 2025, 30(17), 3531; https://doi.org/10.3390/molecules30173531 - 29 Aug 2025
Viewed by 643
Abstract
Dalbergia sissoo is a commercially exploited timber tree also known for its varied phytochemical constituents holding significant importance in folk medicines with documented biological properties. The present study reports the establishment of callus cultures from its leaf explants for the in vitro production [...] Read more.
Dalbergia sissoo is a commercially exploited timber tree also known for its varied phytochemical constituents holding significant importance in folk medicines with documented biological properties. The present study reports the establishment of callus cultures from its leaf explants for the in vitro production of skin therapeutics. The growth parameters of the callus cultures were calculated. The antioxidant potential of the methanolic extracts of leaf and its callus cultures was evaluated through DPPH assay. Calli at third subculture stage showed the highest antioxidant potential (IC50 273 ± 14.14 µg/mL). A comparative analysis of phytochemical composition was performed using Gas Chromatography–Mass Spectrometry (GC-MS) which revealed the presence of potential skin therapeutic compounds. Out of 146 compounds, only 15 are unique to leaf explants, with the rest being produced in callus cultures. ADME predictions of potential compounds showed their drug likeness properties. The molecular docking of selected phytochemicals such as Chondrillasterol, Stearic acid, and n-Hexadecanoic acid against the tyrosinase enzyme showed better binding affinities than the reference drug (Kojic acid). Molecular dynamics simulation also showed stable conformations of the docked complexes with the target protein. Overall, these investigations unveil for the first time the successful in vitro production of skin therapeutics from D. sissoo, ensuring the sustainable and conservation-friendly utilization of its biomass for medicinal purposes. Full article
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