molecules-logo

Journal Browser

Journal Browser

Peptide and Protein-Based Materials: Technology and Applications

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Medicinal Chemistry".

Deadline for manuscript submissions: closed (30 September 2025) | Viewed by 888

Special Issue Editor


E-Mail Website
Guest Editor
Department of Chemical Science and Technologies, University of Rome Tor Vergata, Via della Ricerca Scientifica 1, 00133 Rome, Italy
Interests: peptide and protein nanotechnology; peptide materials; spectroscopy of biomolecules; peptide self-assembly; porphyrin aggregation and nanostructures
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Peptides and proteins are fundamental components of the machinery governing biosystem behavior. To mimic the functional properties and efficiency of these versatile biological engines, the last decade witnessed an explosion of interest in the application of peptides and proteins as smart biomaterials. The advancement of knowledge on peptide and protein technology has been impressive on both the fundamental side (mechanisms of growth of peptide nanostructures, controlled self-assembly of proteins, and rational design of multi-component systems) and its applications (nanocatalysis, biosensing, biohybrid devices, photonics, nanomedicine, tissue engineering, and drug delivery). It is now time for a comprehensive survey on the applications and technological progress in this exciting field. This Special Issue of Molecules, dedicated to “Peptide and Protein-Based materials: Technology and Applications”, aims to delineate the state of the art in this field.

This Special Issue is open to contributions from leading scientists in the nanomedicine, biomaterials science, bionanoengineering, and bionanotechnology fields on the following topics: a) biomedical applications of peptide and protein materials for therapy, prevention, and diagnostics; b) peptides and proteins as functional components of nanostructured materials (nanotubes, nanoparticles, ultrathin films, and nanofibers); c) rational design, properties, and applications of peptide- and protein-based materials; d) peptide–protein hybrid materials; e) peptide and protein networks, interfaces, hydrogels, and supramolecular complexes; and f) computational approaches for the design of peptide and protein nanostructures.

Dr. Mariano Venanzi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bionanotechnology
  • nanobiomedicine
  • peptide materials
  • peptide nanostructures
  • protein materials
  • protein technology

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • Reprint: MDPI Books provides the opportunity to republish successful Special Issues in book format, both online and in print.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

20 pages, 2545 KB  
Article
Impacts of Tween-20, Glycerol, and Trehalose on Hyaluronidase Activity: Insights from Microscale Thermophoresis and Capillary Electrophoresis
by Rouba Nasreddine, Josipa Cecić Vidoš, Alexandra Launay and Reine Nehmé
Molecules 2025, 30(19), 4008; https://doi.org/10.3390/molecules30194008 - 7 Oct 2025
Viewed by 437
Abstract
Additives such as surfactants (Tween-20) and cryoprotectants (glycerol and trehalose) are often used in enzymatic assays to improve the quality and long-term stabilization of proteins. However, these additives can affect the enzymatic activity and the enzyme’s affinity for active compounds, such as inhibitors, [...] Read more.
Additives such as surfactants (Tween-20) and cryoprotectants (glycerol and trehalose) are often used in enzymatic assays to improve the quality and long-term stabilization of proteins. However, these additives can affect the enzymatic activity and the enzyme’s affinity for active compounds, such as inhibitors, and must be considered during assay design since a slight shift in enzyme behavior may compromise the reliability of the results. In this study, the effects of Tween-20, glycerol, and trehalose on hyaluronidase (Hyal) were systematically evaluated by assessing their influence both directly—through microscale thermophoresis (MST) signals of the labeled enzyme (Hyal*)—and indirectly, by monitoring the formation of the final product of the degradation of hyaluronic acid, tetrasaccharide (Tet), using capillary electrophoresis (CE/UV). Hyal was labeled for the first time with ATTO-647 NHS ester, a commercial dye compatible with MST. Efficient labeling was achieved in a phosphate-based buffer without loss of catalytic activity. Tween-20 showed no impact on MST signals nor on enzymatic performance when used between 0.005 and 0.05% (v/v). Glycerol also did not interfere with MST measurements; however, it significantly reduced catalytic activity at concentrations above 2% (v/v). Trehalose affected Hyal* fluorescence in a concentration-dependent manner and enhanced catalytic activity even at 0.02% (v/v). Full article
(This article belongs to the Special Issue Peptide and Protein-Based Materials: Technology and Applications)
Show Figures

Figure 1

Back to TopTop