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Pharmacological Bioassays of Secondary Metabolites of Medicinal Plants

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Natural Products Chemistry".

Deadline for manuscript submissions: 30 April 2026 | Viewed by 467

Special Issue Editors


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Guest Editor
Departamento de Ingeniería Metalúrgica, Facultad de Ingeniería, Universidad de Santiago de Chile, Estación Central, Santiago 9160000, Chile
Interests: medicinal plants; pharmacology of natural products and medicinal plants; ethnobotany

Special Issue Information

Dear Colleagues,

About 20% of the medicinal plants that inhabit our planet have been studied and even less have been studied for their pharmacological properties. Every day, new products of plant origin are discovered, and it is becoming increasingly necessary to understand their mechanisms of action in order to find new drugs to treat both new diseases and others that date back many years. It must be considered that many diseases do not have a treatment drug that has been studied. It is becoming increasingly necessary for many researchers to contribute, in one way or another, to the search for new drugs, new mechanisms of action, and to bring some relief to many people who suffer from the lack of medicines. Pharmacology plays a very important role in the search for a mechanism of action in animals and humans; we must contribute by developing new opportunities so that researchers are attracted to publish their results.

Dr. José L. Martínez
Dr. Luigi Milella
Guest Editors

Manuscript Submission Information

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Keywords

  • pharmacology of medicinal plants
  • pharmacology of natural products
  • medicinal plants
  • mechanisms of action (cardiovascular, nervous system, digestive, etc.)
  • active principles in diseases (Parkinson's, cancer, ulcers, etc.)
  • isolation and bioassays of medicinal plant extracts

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Published Papers (1 paper)

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Research

18 pages, 992 KB  
Article
Potential Antiviral Compounds from Hippeastrum puniceum Bulb Against Yellow Fever Virus: Bioassay-Guided Fractionation and In Silico Pharmacokinetic Analysis
by Eliza Flores-Souza, Alisson Samuel Portes Caldeira, Carolina Colombelli Pacca-Mazaro, Tamiris Vanessa Miguel de Souza, Thaís Magalhães Acácio, Emerson de Castro Barbosa, Naiara Clemente Tavares, Carlos Eduardo Calzavara-Silva, Carlos Leomar Zani, Douglas Eduardo Valente Pires, Tânia Maria de Almeida Alves and Jaquelline Germano de Oliveira
Molecules 2025, 30(21), 4149; https://doi.org/10.3390/molecules30214149 - 22 Oct 2025
Viewed by 210
Abstract
Despite the availability of effective vaccines, yellow fever outbreaks persist, highlighting the need for antiviral drugs. Background/Objectives: This study investigated Hippeastrum puniceum (Amaryllidaceae) as a potential source of antiviral compounds against wild-type yellow fever virus (wt-YFV). Methods/Results: The crude bulb extract of H. [...] Read more.
Despite the availability of effective vaccines, yellow fever outbreaks persist, highlighting the need for antiviral drugs. Background/Objectives: This study investigated Hippeastrum puniceum (Amaryllidaceae) as a potential source of antiviral compounds against wild-type yellow fever virus (wt-YFV). Methods/Results: The crude bulb extract of H. puniceum exhibited 58% protection against wt-YFV. Bioassay-guided fractionation of the extract by UHPLC-HRMS led to the annotation of six alkaloids (bulbisine, cathinone, trigonelline, tetrahydroharman-3-carboxylic acid, and 2,7-dimethoxyhomolycorine or 3-O-acetylnarcissidine) in active fractions, along with the amino acids arginine, asparagine, tryptophan, and glutamic acid. In silico ADMET analyses predicted favorable pharmacokinetic and toxicological profiles, supporting their potential as drug candidates. Six of the annotated compounds were evaluated in vitro for cytotoxicity and antiviral activity against wt-YFV. However, none showed significant antiviral activity when tested individually, suggesting that the observed antiviral effect may result from synergistic interactions between two or more compounds within active fractions. Conclusions: Our results underscore the importance of further investigations in vitro, particularly assays exploring the synergy among the annotated compounds against YFV. The integration of bioassay-guided fractionation of active plant extracts with computational analyses emerges as a promising strategy for the discovery of natural products with therapeutic potential against yellow fever, a reemerging disease. Full article
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