E-Mail Alert

Add your e-mail address to receive forthcoming issues of this journal:

Journal Browser

Journal Browser

Special Issue "Antisense Oligonucleotide Chemistry and Applications"

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Bioorganic Chemistry".

Deadline for manuscript submissions: 15 May 2019

Special Issue Editor

Guest Editor
Dr. Rakesh N. Veedu

Centre for Comparative Genomics & Perron Institute for Neurological & Translational Science, Murodch University, Perth, Australia
Website | E-Mail
Interests: antisense oligonucleotides; aptamers; DNAzymes; antimiRs; chimeric nucleic acids; chemically-modified oligonucleotides

Special Issue Information

Dear Colleagues,

Synthetic antisense oligonucleotide (AO)-based therapy has attracted significant interests in the last decade, and offers enormous potential as therapeutics to various diseases. To date, five AO drugs have been approved by the US Food and Drug Administration (FDA) for clinical use including Vitravene for cytomegalovirus retinitis; Kynamro for familial hypercholesterolemia, Exondys51 for the treatment of Duchenne muscular dystrophy, Spinraza for the treatment of spinal muscular atrophy and very recently Tegsedi for Hereditary Transthyretin Amyloidosis. As you all know from these clinical translations that the use of chemically-modified nucleotide monomers in constructing AOs is absolutely essential in developing successful drugs.

This special issue covers various aspects of antisense oligonucleotide synthesis chemistries, and their applications in molecular medicine in the form of authentic research and review articles.

Dr. Rakesh N. Veedu
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access bimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Antisense Oligonucleotides
  • Antisense Therapy
  • Chemically-modified Antisense Oligonucleotides
  • Exon-skipping
  • Nucleic Acid Chemistry

Published Papers (1 paper)

View options order results:
result details:
Displaying articles 1-1
Export citation of selected articles as:

Research

Open AccessFeature PaperArticle Systematic Screening of Commonly Used Commercial Transfection Reagents towards Efficient Transfection of Single-Stranded Oligonucleotides
Molecules 2018, 23(10), 2564; https://doi.org/10.3390/molecules23102564
Received: 10 September 2018 / Revised: 4 October 2018 / Accepted: 5 October 2018 / Published: 8 October 2018
PDF Full-text (7635 KB) | HTML Full-text | XML Full-text | Supplementary Files
Abstract
Non-viral vector-mediated transfection is a core technique for in vitro screening of oligonucleotides. Despite the growing interests in the development of oliogonucleotide-based drug molecules in recent years, a comprehensive comparison of the transfection efficacy of commonly used commercial transfection reagents has not been
[...] Read more.
Non-viral vector-mediated transfection is a core technique for in vitro screening of oligonucleotides. Despite the growing interests in the development of oliogonucleotide-based drug molecules in recent years, a comprehensive comparison of the transfection efficacy of commonly used commercial transfection reagents has not been reported. In this study, five commonly used transfection reagents, including Lipofectamine 3000, Lipofectamine 2000, Fugene, RNAiMAX and Lipofectin, were comprehensively analyzed in ten cell lines using a fluorescence imaging-based transfection assay. Although the transfection efficacy and toxicity of transfection reagents varied depending on cell types, the toxicity of transfection reagents generally displayed a positive correlation with their transfection efficacy. According to our results, Lipofectamine 3000, Fugene and RNAiMAX showed high transfection efficacy, however, RNAiMAX may be a better option for majority of cells when lower toxicity is desired. The transfection efficacy of Lipofectamine 2000 was compromised by its high toxicity, which may adversely affect its application in most cells. We firmly believe that our findings may contribute to the future In vitro delivery and screening of single-stranded therapeutic oligonucleotides such as antisense oligonucleotides, antimiRs, and DNAzymes. Full article
(This article belongs to the Special Issue Antisense Oligonucleotide Chemistry and Applications)
Figures

Figure 1

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top