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Stable Isotope Techniques Across Chemistry: Methods, Applications, and Perspectives

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 31 March 2026 | Viewed by 250

Special Issue Editor


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Guest Editor
Fondazione Edmund Mach, Via E. Mach 1, 38098 San Michele all’Adige, Italy
Interests: stable isotope analysis; traceability; authentication; mass spectrometry; method development

Special Issue Information

Dear Colleagues,

The stable isotopic composition of matter is governed by a wide range of physical, chemical, and biological mechanisms. Tracking the different transformations of such matter has become possible through the use of stable isotope analysis. This powerful tool provides insights into processes occurring at various spatial and temporal scales. Over the last decades, the development of increasingly accurate and sophisticated isotopic techniques has enabled the analysis of an exceptionally broad spectrum of matrices, including environmental (e.g., sediments, soils, waters, petroleum, meteorites), biological (e.g., bones, blood, organic tissues), and agri-food samples (e.g., milk, oils, wine). As a result, the application of stable isotope methodologies has rapidly expanded across numerous scientific disciplines, including geochemistry, archaeology, forensic science, biomedicine, environmental science, microbiology, soil science, and doping and fraud control, among many others. Indeed, the versatility and robustness of isotopic approaches make them a unique tool, with virtually limitless potential for unveiling new knowledge across the natural and applied sciences.

This Special Issue is dedicated to providing an updated and comprehensive view of recent advances in the application of stable isotope analysis within the diverse fields of chemistry. Contributions may include, but are not limited to, improvements in analytical procedures and instrumentation, such as new sample preparation strategies and innovative instrumental setups, as well as novel and emerging applications of isotope-based methods. Furthermore, studies that showcase recent applications of already well-established methodologies, yielding significant and novel results, are highly encouraged, along with review articles that synthesize current knowledge and provide future perspectives.

We are confident that this collection will be of great interest to the scientific community, reflecting the growing relevance and expanding role of isotopic analysis as a cornerstone in modern chemical and interdisciplinary research.

Dr. Matteo Perini
Guest Editor

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • stable isotope analysis
  • compound specific stable isotopes
  • authenticity
  • traceability
  • biogenic isotopic composition
  • biogeochemistry
  • geochemistry
  • molecular archaeometry
  • environmental chemistry

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Published Papers (1 paper)

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Research

13 pages, 435 KB  
Article
Influence of Diet and Growth Conditions on the Carbon and Nitrogen Stable Isotopic Composition of Aspergillus niger Mycelium: Insights for Fungal Chitosan Characterization
by Matteo Perini, Raffaele Guzzon, Silvia Pianezze, Francesca Violardo and Roberto Larcher
Molecules 2025, 30(20), 4142; https://doi.org/10.3390/molecules30204142 - 21 Oct 2025
Viewed by 164
Abstract
This study investigates, for the first time, the relationship between carbon (δ13C) and nitrogen stable isotopic composition of Aspergillus niger mycelium, used as chitin and chitosan sources, and the fungus diet under controlled cultivation conditions. Four diets were tested, combining [...] Read more.
This study investigates, for the first time, the relationship between carbon (δ13C) and nitrogen stable isotopic composition of Aspergillus niger mycelium, used as chitin and chitosan sources, and the fungus diet under controlled cultivation conditions. Four diets were tested, combining different carbon (C3- and C4-glucose) and nitrogen sources (KNO3 and NH4Cl). Results showed that carbon sources significantly influenced δ13C values of the mycelium: C4-glucose diets led to more negative Δ13C values (δ13CMYCELIUM-δ13CDIET) compared to C3-glucose diets. Nitrogen sources also affected isotopic fractionation, with KNO3 leading to negative Δ15N (δ15NMYCELIUM-δ15NDIET) and NH4Cl yielding positive Δ15N. Conversely, pH and temperature showed negligible effects on δ15N, while continuous aeration during growth significantly decreased δ15N, possibly due to partial assimilation of atmospheric nitrogen. These findings demonstrate that both nutrient and cultivation parameters can modulate the isotopic fractionation in A. niger, particularly for nitrogen. Although a direct correlation between diet composition and δ15N could not be established, this work provides the first experimental link between fungal metabolism and its isotopic fingerprint. The results offer a scientific foundation for applying stable isotope ratio analysis to authenticate and trace fungal-derived chitin and chitosan, with potential applications in food and winemaking industries. Full article
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