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Microorganisms
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Pseudomonas aeruginosa: From Infection Biology to Clinical Management
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Pseudomonas aeruginosa: From Infection Biology to Clinical Management

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Medical Microbiology".

Deadline for manuscript submissions: closed (31 October 2020) | Viewed by 7924

Special Issue Editor

Prof. Dr. Teiji Sawa

E-Mail Website
Guest Editor
Department of Anesthesiology, Kyoto Prefectural University of Medicine, Kyoto 602‐8566, Japan
Interests: Pseudomonas aeruginosa; microbiology; pneumonia; Gram-negative bacteria; PcrV; immunology; infection

Special Issue Information

Dear Colleagues,

The global spread of multidrug-resistant Pseudomonas aeruginosa strains is hazardous for immunocompromised patients because this pathogen is associated with morbidity and mortality. This growing threat has a strong association with adaptation mechanisms possessed by P. aeruginosa. Multiple virulence factors, such as secreted toxins, lipopolysaccharides, flagella, and biofilm, contribute to the pathogenesis of this bacterium. In addition, its extraordinary capacity for developing drug resistance is critical for clinicians to treat the lethal infections caused by P. aeruginosa. It is an on-going road for the development of prevention and therapeutics in P. aeruginosa infections to understand the biology of P. aeruginosa and the subsequent pathogenesis regarding virulence mechanisms that lead to acute lung injury, bacteremia, and sepsis. This Special Issue about P. aeruginosa targets researchers and clinicians who are daily tackling infections caused by this bacterium, from both research and clinical aspects.

For this purpose, we cordially invite authors to submit research articles, review articles, and short communications related to the various aspects of Pseudomonas aeruginosa: bacteriology, epidemiology, antimicrobial agents, immunotherapeutics, and clinical case reports.

Prof. Dr. Teiji Sawa
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Pseudomonas aeruginosa
  • toxins
  • human infection
  • foodborne diseases
  • microbiology
  • antimicrobial agents
  • antimicrobial resistance
  • bacteria–host interactions
  • epidemiology
  • diagnostic methods
  • prevention
  • therapeutics
  • vaccines

Published Papers (2 papers)

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Research

22 pages, 3709 KiB  
Article
Occurrence of blaTEM and blaCTXM Genes and Biofilm-Forming Ability among Clinical Isolates of Pseudomonas aeruginosa and Acinetobacter baumannii in Yaoundé, Cameroon
by Estelle Longla Madaha, Hortense Kamga Gonsu, Rhoda Nsen Bughe, Marie Christine Fonkoua, Collins Njie Ateba and Wilfred Fon Mbacham
Microorganisms 2020, 8(5), 708; https://doi.org/10.3390/microorganisms8050708 - 11 May 2020
Cited by 9 | Viewed by 3162
Abstract
Background: Pseudomonas aeruginosa (PSA) and Acinetobacter baumannii (ACB) are non-fermentative bacteria mostly associated with nosocomial infections in humans. Objective: This study aimed to determine the antimicrobial resistance profiles and virulence gene of PSA and ACB previously isolated from humans in selected health facilities [...] Read more.
Background: Pseudomonas aeruginosa (PSA) and Acinetobacter baumannii (ACB) are non-fermentative bacteria mostly associated with nosocomial infections in humans. Objective: This study aimed to determine the antimicrobial resistance profiles and virulence gene of PSA and ACB previously isolated from humans in selected health facilities in Yaoundé, Cameroon. Methods: A total of 77 and 27 presumptive PSA and ACB isolates, respectively, were collected from the Yaoundé teaching hospital. These isolates were previously isolated from various samples including pus, blood and broncho-alveolar lavage. The identities of the isolates were determined through polymerase chain reaction (PCR) amplification of PSA and ACB specific sequences. Antimicrobial susceptibility testing (AST) was performed using the Kirby–Bauer disc diffusion method. Phenotypical expression of AmpC β-lactamases (AmpC), extended spectrum β-lactamases (ESBLs) and metallo β-Lactamases (MBLs) were determined using the combined disc method. Bacterial genomes were screened for the presence of β-lactamases blaTEM and blaCTXM genes using specific PCR. The pathogenicity of PSA and ACB was assessed through amplification of the lasB, exoA, pslA and exoS as well as OmpA and csuE virulence genes, respectively. Results: Of the 77 presumptive PSA isolates, a large proportion (75 to 97.4%) were positively identified. All (100%) of the presumptive 27 ACB harbored the ACB-specific ITS gene fragment by PCR. Twenty five percent of the PSA isolates produced ESBLs phenotypically while more than 90% of these isolates were positive for the lasB, exoA, pslA and exoS genes. A large proportion (88%) of the ACB isolates harboured the OmpA and csuE genes. blaTEM and blaCTXM were detected in 17 and 4% of PSA, respectively, while a much higher proportion (70 and 29%) of the ACB isolates possessed these resistance determinants respectively. Conclusion: Our findings reveal the occurrence of both virulence and drug-resistant determinants in clinical PSA and ACB isolates from patients in health care settings in Yaoundé, Cameroon, thus suggesting their role in the pathological conditions in patients. Full article
(This article belongs to the Special Issue Pseudomonas aeruginosa: From Infection Biology to Clinical Management)
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16 pages, 1216 KiB  
Article
Propolis Affects Pseudomonas aeruginosa Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols
by Aida Meto, Bruna Colombari, Agron Meto, Giorgia Boaretto, Diego Pinetti, Lucia Marchetti, Stefania Benvenuti, Federica Pellati and Elisabetta Blasi
Microorganisms 2020, 8(2), 243; https://doi.org/10.3390/microorganisms8020243 - 12 Feb 2020
Cited by 34 | Viewed by 4265
Abstract
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen responsible for a wide range of clinical conditions, from mild infections to life-threatening nosocomial biofilm-associated diseases, which are particularly severe in susceptible individuals. The aim of this in vitro study was to assess [...] Read more.
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen responsible for a wide range of clinical conditions, from mild infections to life-threatening nosocomial biofilm-associated diseases, which are particularly severe in susceptible individuals. The aim of this in vitro study was to assess the effects of an Albanian propolis on several virulence-related factors of P. aeruginosa, such as growth ability, biofilm formation, extracellular DNA (eDNA) release and phenazine production. To this end, propolis was processed using three different solvents and the extracted polyphenolic compounds were identified by means of high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis. As assessed by a bioluminescence-based assay, among the three propolis extracts, the ethanol (EtOH) extract was the most effective in inhibiting both microbial growth and biofilm formation, followed by propylene glycol (PG) and polyethylene glycol 400 (PEG 400) propolis extracts. Furthermore, Pseudomonas exposure to propolis EtOH extract caused a decrease in eDNA release and phenazine production. Finally, caffeic acid phenethyl ester (CAPE) and quercetin decreased upon propolis EtOH extract exposure to bacteria. Overall, our data add new insights on the anti-microbial properties of a natural compound, such as propolis against P. aeruginosa. The potential implications of these findings will be discussed. Full article
(This article belongs to the Special Issue Pseudomonas aeruginosa: From Infection Biology to Clinical Management)
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