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Article

Propolis Affects Pseudomonas aeruginosa Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols

1
School of Doctorate in Clinical and Experimental Medicine, University of Modena and Reggio Emilia, Via G. Campi 287-103, 41125 Modena, Italy
2
Department of Surgery, Medicine, Dentistry and Morphological Sciences with interest in Transplant, Oncology and Regenerative Medicine, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy
3
Department of Therapy, Faculty of Dental Medicine, University of Aldent, Rr. e Dibres 235, 1000 Tirana, Albania
4
School of Specialization in Microbiology and Virology, University of Modena and Reggio Emilia, Via G. Campi 287, 41125 Modena, Italy
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Centro Interdipartimentale Grandi Strumenti, University of Modena and Reggio Emilia, via Campi 213/A, 41125 Modena, Italy
6
Department of Life Sciences, University of Modena and Reggio Emilia, Via G. Campi 103, 41125 Modena, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Microorganisms 2020, 8(2), 243; https://doi.org/10.3390/microorganisms8020243
Received: 18 December 2019 / Revised: 7 February 2020 / Accepted: 10 February 2020 / Published: 12 February 2020
(This article belongs to the Special Issue Pseudomonas aeruginosa: From Infection Biology to Clinical Management)
Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen responsible for a wide range of clinical conditions, from mild infections to life-threatening nosocomial biofilm-associated diseases, which are particularly severe in susceptible individuals. The aim of this in vitro study was to assess the effects of an Albanian propolis on several virulence-related factors of P. aeruginosa, such as growth ability, biofilm formation, extracellular DNA (eDNA) release and phenazine production. To this end, propolis was processed using three different solvents and the extracted polyphenolic compounds were identified by means of high performance liquid chromatography coupled to electrospray ionization mass spectrometry (HPLC-ESI-MS) analysis. As assessed by a bioluminescence-based assay, among the three propolis extracts, the ethanol (EtOH) extract was the most effective in inhibiting both microbial growth and biofilm formation, followed by propylene glycol (PG) and polyethylene glycol 400 (PEG 400) propolis extracts. Furthermore, Pseudomonas exposure to propolis EtOH extract caused a decrease in eDNA release and phenazine production. Finally, caffeic acid phenethyl ester (CAPE) and quercetin decreased upon propolis EtOH extract exposure to bacteria. Overall, our data add new insights on the anti-microbial properties of a natural compound, such as propolis against P. aeruginosa. The potential implications of these findings will be discussed. View Full-Text
Keywords: P. aeruginosa; virulence factors; phenazines; eDNA; MIC; antimicrobial; antibiofilm; propolis; polyphenols P. aeruginosa; virulence factors; phenazines; eDNA; MIC; antimicrobial; antibiofilm; propolis; polyphenols
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MDPI and ACS Style

Meto, A.; Colombari, B.; Meto, A.; Boaretto, G.; Pinetti, D.; Marchetti, L.; Benvenuti, S.; Pellati, F.; Blasi, E. Propolis Affects Pseudomonas aeruginosa Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols. Microorganisms 2020, 8, 243. https://doi.org/10.3390/microorganisms8020243

AMA Style

Meto A, Colombari B, Meto A, Boaretto G, Pinetti D, Marchetti L, Benvenuti S, Pellati F, Blasi E. Propolis Affects Pseudomonas aeruginosa Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols. Microorganisms. 2020; 8(2):243. https://doi.org/10.3390/microorganisms8020243

Chicago/Turabian Style

Meto, Aida, Bruna Colombari, Agron Meto, Giorgia Boaretto, Diego Pinetti, Lucia Marchetti, Stefania Benvenuti, Federica Pellati, and Elisabetta Blasi. 2020. "Propolis Affects Pseudomonas aeruginosa Growth, Biofilm Formation, eDNA Release and Phenazine Production: Potential Involvement of Polyphenols" Microorganisms 8, no. 2: 243. https://doi.org/10.3390/microorganisms8020243

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