Probiotics: Identification and Applications

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Microbial Biotechnology".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 877

Special Issue Editors


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Guest Editor
Facultad de Medicina Mexicali, Universidad Autónoma de Baja California, Mexicali 21000, Mexico
Interests: probiotics; functional products with health applications

E-Mail Website
Guest Editor
Laboratorio de Biotecnologia, Departamento de Sistemas Biologicos, Universidad Autonoma Metropolitana Campus Xochimilco, Calzada del Hueso 1100, Col. Villa Quietud, Alcaldia Coyoacan, Mexico City 04960, Mexico
Interests: probiotics; gut microbiota

Special Issue Information

Dear Colleagues,

Research on probiotics has grown significantly worldwide. Initially focused on gastrointestinal issues, advances in metagenomics have broadened our understanding of their roles across various niches, including the gut, skin, and vaginal microbiomes. These studies have led to the identification of both traditional and next-generation probiotic strains with specific functions, such as producing short-chain fatty acids and metabolites with diverse effects (e.g., antioxidant, anti-cancer, and psychobiotic activities). Promising candidates encompassing Akkermansia muciniphila and Faecalibacterium prausnitziis show potential in metabolic health and inflammation control, while Lactobacillus crispatus and Lactobacillus reuteri have demonstrated efficacy in managing urogenital infections. Psychobiotics, including Lactobacillus rhamnosus and Bifidobacterium longum, highlight the potential of probiotics in modulating mental health through the gut–brain axis. Probiotic research has also expanded into oncology, where specific strains show promise in immune modulation and support during cancer therapies.

Emerging areas include the development of postbiotics—bioactive metabolites produced by probiotics—and synbiotics, which combine probiotics and prebiotics to enhance their functionality. Fermented foods continue to gain attention as natural sources of probiotics and postbiotics, contributing to microbiome diversity and overall health.

This Special Issue invites reviews, perspectives, and original research articles on new probiotic strains from human and unconventional sources (e.g., fermented foods). Topics of interest include probiotic applications in gastrointestinal disorders (e.g., IBD, IBS), urogenital diseases, cancer, food matrices, interactions with prebiotics, postbiotic and psychobiotic effects, and the elucidation of mechanisms of action. We particularly encourage studies on novel probiotic strains and their functional applications.

Dr. Edgar Torres-Maravilla
Prof. Dr. Lino Reyes
Guest Editors

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Keywords

  • bifidobacteria
  • fermented food
  • functional characterization
  • genome
  • health benefit
  • lactic acid bacteria
  • probiotic
  • postbiotic
  • psychobiotic

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Published Papers (1 paper)

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Research

23 pages, 6419 KiB  
Article
The Genomic Characteristics of Potential Probiotics: Two Streptococcus salivarius Isolates from a Healthy Individual in China
by Mingyue Sun, Qian Li, Feiran Zhang, Ding Yao, Wenhua Huang, Qingyu Lv, Hua Jiang, Decong Kong, Yuhao Ren, Shaolong Chen, Yongqiang Jiang and Peng Liu
Microorganisms 2025, 13(3), 694; https://doi.org/10.3390/microorganisms13030694 - 20 Mar 2025
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Abstract
The isolation and characterization of novel probiotics from dairy products, fermented foods, and the gut have gained significant attention. In particular, Streptococcus salivarius shows promise for use in oral probiotic preparations. In this study, we isolated two strains of S. salivarius—S.82.15 and [...] Read more.
The isolation and characterization of novel probiotics from dairy products, fermented foods, and the gut have gained significant attention. In particular, Streptococcus salivarius shows promise for use in oral probiotic preparations. In this study, we isolated two strains of S. salivarius—S.82.15 and S.82.20—from the oral cavity of a healthy individual. These strains exhibited distinct antimicrobial profiles. We thoroughly assessed the morphology and growth patterns of both strains and confirmed auto-aggregation and hemolytic activity. Through comprehensive genomic analysis, we found notable strain differences within the same bacterial species isolated from the same individual. Notably, the presence or absence of plasmids varied between the two strains. The genome of S.82.15 spans 2,175,688 bps and contains 1994 coding DNA sequences (CDSs), while S.82.20 has a genome size of 2,414,610 bps, a GC content of 40.62%, and 2276 annotated CDSs. Both strains demonstrated antibacterial activity against Group A Streptococcus (GAS), Micrococcus. luteus, and Porphyromonas gingivalis. To investigate the antibacterial properties further, we identified a gene cluster of salivaricin 9 on the plasmid of S.82.20 and a blp gene family on the chromosomes of both S.82.15 and S.82.20. Moreover, the gene expression of the blp family was upregulated when the isolated strains were co-cultured with GAS. Full article
(This article belongs to the Special Issue Probiotics: Identification and Applications)
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