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Microorganisms
Special Issues
Advances in Brucella
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Advances in Brucella

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Medical Microbiology".

Deadline for manuscript submissions: closed (30 April 2025) | Viewed by 4932

Special Issue Editor

Prof. Dr. Angel Oñate

E-Mail Website
Guest Editor
Departamento de Microbiología, Facultad de Ciencias Biológicas, Universidad de Concepción, Concepción 4070386, Chile
Interests: Brucella; immunogenicity

Special Issue Information

Dear Colleagues,

Brucella, which is a Gram-negative bacterium with a high infective capacity and a wide spectrum of potential hosts in the animal world, is found in both terrestrial and marine mammals, as well as in amphibians. Brucellosis, which is caused by the facultative intracellular bacteria of the Brucella species, is one the most globally prevalent examples of a zoonosis.

For this Special Issue, we invite authors to submit research articles, review articles, and written publications that cover any aspect of the Brucella species’ relevance to the fields of bacteriology, epidemiology, molecular biology, genomics, and biofilms. Publications that cover diagnostics, pathogenesis, vaccine research, or surveillance measures related to Brucellosis are also welcome. We look forward to receiving your contribution.

Prof. Dr. Angel Oñate
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

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Research

23 pages, 3088 KiB  
Article
Ghostbuster—A Western Blot-Based Panel Method to Resolve False-Positive Brucellosis Serology Test Results
by Borbála Bányász, József Antal and Béla Dénes
Microorganisms 2025, 13(3), 574; https://doi.org/10.3390/microorganisms13030574 - 3 Mar 2025
Viewed by 826
Abstract
False-positive serologic results (FPSRs) of brucellosis occur from time to time in various livestock with all the consequences (quarantine, compulsory slaughter, etc.) that follow true-positive laboratory results. A method based on the Polyacrylamide Gel Electrophoresis/Western Blot of a protein panel for resolving the [...] Read more.
False-positive serologic results (FPSRs) of brucellosis occur from time to time in various livestock with all the consequences (quarantine, compulsory slaughter, etc.) that follow true-positive laboratory results. A method based on the Polyacrylamide Gel Electrophoresis/Western Blot of a protein panel for resolving the FPSRs in the diagnosis of brucellosis was developed. Within the context of limited positive serum sample availability in Europe, the method successfully discriminates Brucella-positive sera from samples containing antibodies raised against infections caused by other Gram-negative bacteria causing FPSRs. An average CV% of 1.36 was determined for both repeatability and reproducibility for the whole separation mw range, and the test achieved 1.00 Diagnostic Sensitivity and 1.00 Diagnostic Specificity. The method with pre-prepared WB panels provides a rapid (less than 3 h), easily standardizable, and validatable alternative to existing confirmation methods. The whole WB process of the Brucella proteins and the subsequent densitometry can be accomplished with commercially available equipment, ready-to-use reagents, and open-source software, providing cost-effectiveness. The results of this study could attract broader attention, since molecular species in the 35.0–75.0 kDa range can serve as antigens in Brucella serology and the same fraction can be considered in the development of synthetic Brucella vaccines. Full article
(This article belongs to the Special Issue Advances in Brucella)
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17 pages, 8395 KiB  
Article
Polyphasic Characterization of Brucella spp. in Livestock Slaughtered from Abattoirs in Eastern Cape, South Africa
by Koketso Desiree Mazwi, Francis Babaman Kolo, Ishmael Festus Jaja, Charles Byaruhanga, Ayesha Hassim and Henriette van Heerden
Microorganisms 2024, 12(1), 223; https://doi.org/10.3390/microorganisms12010223 - 22 Jan 2024
Cited by 3 | Viewed by 2487
Abstract
In livestock, brucellosis is mainly an asymptomatic disease except when abortion occurs; therefore, two serological tests are used for diagnosis as no single test is suitable. Abattoir samples enable a combination of culture, molecular, and serological tests to detect brucellosis. This study assessed [...] Read more.
In livestock, brucellosis is mainly an asymptomatic disease except when abortion occurs; therefore, two serological tests are used for diagnosis as no single test is suitable. Abattoir samples enable a combination of culture, molecular, and serological tests to detect brucellosis. This study assessed Brucella-specific PCR (ITS-PCR) to detect brucellosis and to conduct a molecular characterization of Brucella spp. isolated from PCR-positive livestock (n = 565) slaughtered at abattoirs and the appropriate sample tissue(s). ITS-PCR detected Brucella DNA in 33.6% of cattle, 14.5% of sheep, and 4.7% of pig tissues. Impure Brucella cultures from PCR-positive tissues were 43.6% (44/94) of cattle, 51.7% (15/29) of sheep, and 50% (2/4) of pigs with predominantly B. abortus identification with AMOS-PCR and low isolation of mixed B. abortus and B. melitensis in all species. In cattle, 33% of isolates were from lymph nodes, while in sheep 38.0% were from the liver and kidney and only from tonsils in pigs (2/4). Brucella infections identified with AMOS-PCR were present in seropositive and mainly seronegative (75.6–100%) livestock with the potential to cause brucellosis during pregnancy or breeding. This study demonstrated the value of the polyphasic approach, especially with chronic infections and the potential risk of these asymptomatic animals. Full article
(This article belongs to the Special Issue Advances in Brucella)
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