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Microorganisms
Volume 13
Issue 7
10.3390/microorganisms13071442
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Brief Report

Enhanced Isolation of Brucella abortus from Lymphoid Tissues of Mice Orally Infected with Low Doses in a Two-Step Procedure

by
Ana Beatriz Sánchez-Argáez
1,2,
Estefania Herrera-Torres
1,
Martha Cecilia Moreno-Lafont
1,†,
Leopoldo Flores-Romo
2 and
Rubén López-Santiago
1,*
1
Departamento de Inmunología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. de Carpio y Plan de Ayala s/n, Del. Miguel Hidalgo, Ciudad de México 11340, Mexico
2
Departamento de Biomedicina Molecular y Departamento de Biología Celular, Centro de Investigación y de Estudios Avanzados (CINVESTAV), Instituto Politécnico Nacional, Av. IPN No. 2508. Del. Gustavo A. Madero, Ciudad de México 07360, Mexico
*
Author to whom correspondence should be addressed.
†
Deceased author.
Microorganisms 2025, 13(7), 1442; https://doi.org/10.3390/microorganisms13071442
Submission received: 11 April 2025 / Revised: 2 June 2025 / Accepted: 17 June 2025 / Published: 20 June 2025
(This article belongs to the Special Issue Advances in Brucella)
Versions Notes

Abstract

The main aspects of brucellosis have been studied in animal models to better understand the pathogenesis of the disease. Mice are the most common animal model of brucellosis. To verify that the infection has been successfully induced, it is necessary to assess the presence of Brucella in experimentally infected mice. Traditionally, high doses of Brucella have been used to establish detectable infection in oral murine models but prevent the emulation of natural pathogenesis. We propose the use of a low dose (1 × 106 CFUs) to establish a more realistic oral infection model. Using a two-step procedure consisting of selective broth enrichment followed by agar isolation, we were able to recover bacteria from gut-associated lymphoid tissues (mesenteric lymph nodes and Peyer’s patches), the spleen, and feces during the early and late stages of infection (1 h and up to 5 weeks). This technique promotes the study of early infection stages and systemic dissemination without the need for high doses to induce infection orally. It also demonstrates that Brucella remains in the intestinal-associated lymphoid tissues at time points when the infection is already systemically established.
Keywords: Brucella abortus; oral infection; mouse models; isolation of Brucella Brucella abortus; oral infection; mouse models; isolation of Brucella

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MDPI and ACS Style

Sánchez-Argáez, A.B.; Herrera-Torres, E.; Moreno-Lafont, M.C.; Flores-Romo, L.; López-Santiago, R. Enhanced Isolation of Brucella abortus from Lymphoid Tissues of Mice Orally Infected with Low Doses in a Two-Step Procedure. Microorganisms 2025, 13, 1442. https://doi.org/10.3390/microorganisms13071442

AMA Style

Sánchez-Argáez AB, Herrera-Torres E, Moreno-Lafont MC, Flores-Romo L, López-Santiago R. Enhanced Isolation of Brucella abortus from Lymphoid Tissues of Mice Orally Infected with Low Doses in a Two-Step Procedure. Microorganisms. 2025; 13(7):1442. https://doi.org/10.3390/microorganisms13071442

Chicago/Turabian Style

Sánchez-Argáez, Ana Beatriz, Estefania Herrera-Torres, Martha Cecilia Moreno-Lafont, Leopoldo Flores-Romo, and Rubén López-Santiago. 2025. "Enhanced Isolation of Brucella abortus from Lymphoid Tissues of Mice Orally Infected with Low Doses in a Two-Step Procedure" Microorganisms 13, no. 7: 1442. https://doi.org/10.3390/microorganisms13071442

APA Style

Sánchez-Argáez, A. B., Herrera-Torres, E., Moreno-Lafont, M. C., Flores-Romo, L., & López-Santiago, R. (2025). Enhanced Isolation of Brucella abortus from Lymphoid Tissues of Mice Orally Infected with Low Doses in a Two-Step Procedure. Microorganisms, 13(7), 1442. https://doi.org/10.3390/microorganisms13071442

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