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Microorganisms
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Veterinary Microbiology and Diagnostics
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Veterinary Microbiology and Diagnostics

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Veterinary Microbiology".

Deadline for manuscript submissions: closed (15 March 2025) | Viewed by 3532

Special Issue Editor

Dr. Seyed Ali Ghorashi

E-Mail Website
Guest Editor
1. School of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
2. Gulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
Interests: molecular diagnosis and genotyping of veterinary pathogens; epidemiology of infectious diseases
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This special issue is a collaboration between Microorganisms and Microbiology Research, focusing on the theme “Veterinary Microbiology and Diagnostics”. Recognising the crucial role of microbiology and diagnostics in veterinary science, we aim to present a comprehensive collection of high-quality research papers, reviews, and short communications in this specialised discipline.

This Special Issue on “Veterinary Microbiology and Diagnostics” is committed to highlighting cutting-edge developments in the field. We invite veterinary professionals, researchers, and scholars to contribute their original work, spanning a diverse array of topics including infectious diseases, genotyping of veterinary pathogens, antimicrobial resistance, cutting-edge and rapid diagnostic techniques, surveillance, monitoring of veterinary pathogens, and emerging trends. This initiative serves as a collaborative platform, fostering cooperation and facilitating the exchange of knowledge among professionals and researchers deeply engaged in veterinary microbiology. By addressing critical aspects of the field, from molecular characterisations to innovative diagnostic tools and beyond, we aim to create a comprehensive collection that advances our understanding and applications of veterinary microbiology and diagnostics. Authors are encouraged to contribute their expertise, driving the dialogue forward and enhancing the collective knowledge base in this field. Join us in this effort to shape the future of veterinary microbiology and diagnostics through impactful research and collaborative exploration.

You may choose our Joint Special Issue in the journal of Microbiology Research.

Dr. Seyed Ali Ghorashi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microorganisms is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • infectious diseases
  • antimicrobial resistance
  • diagnostic techniques
  • genotyping
  • emerging trends
  • zoonotic diseases
  • vaccination
  • pathogen genomics
  • molecular diagnostics
  • One Health
  • rapid diagnostics
  • veterinary pathogens
  • wildlife microbiology

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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11 pages, 593 KiB  
Article
Application of Amplicon Metagenomics to Identify Fungal Pathogens in Formalin-Fixed Paraffin-Embedded Samples: Proof of Concept in Animals with Fungal Pathologies
by David B. Needle, Guillaume Reboul, Patrick K. Mitchell, Derek Rothenheber, Nicholas J. Marra, Brittany D. Cronk, Neeti G. Patel and Laura B. Goodman
Microorganisms 2025, 13(3), 533; https://doi.org/10.3390/microorganisms13030533 - 27 Feb 2025
Viewed by 440
Abstract
The identification of fungal pathogens in formalin-fixed paraffin-embedded (FFPE) tissues is an unmet need in human and animal medicine, and sequence-agnostic approaches are needed to identify emerging pathogens. Eleven FFPE biopsy specimens with etiologic diagnoses of fungal disease based on standard testing of [...] Read more.
The identification of fungal pathogens in formalin-fixed paraffin-embedded (FFPE) tissues is an unmet need in human and animal medicine, and sequence-agnostic approaches are needed to identify emerging pathogens. Eleven FFPE biopsy specimens with etiologic diagnoses of fungal disease based on standard testing of paired fresh tissue samples were utilized here to evaluate metabarcoding approaches. The cases included tissues from three dogs, three cats, one box turtle, one goat, one common loon, and one gray tree frog. The diagnoses from the fresh tissues in these cases were Microsporum canis, Penicillium sp., Exophiala sp. (likely E. jeanselmei), Verticillium sp., Rhizopus sp., atypical Cryptococcus neoformans, Conidiobolus spp., Aspergillus fumigatus, Cryptococcus neoformans var grubii, Batrachochytrium dendrobatidis, Fusarium solani, Blastomyces dermatitidis, Coccidiodes immitis, and Histoplasma capsulatum. We compared the ITS1 and 28S D1 rRNA gene genetic markers in combination with several bioinformatic strategies to identify fungal pathogens in the FFPE tissue samples, with a success rate of 9/11. These methods could allow diagnosticians who receive only FFPE tissues and see fungal pathogens to speciate the pathogens and could be of value in retrospective studies wherein FFPE tissue is the only archived tissue. Furthermore, these techniques could be of use to researchers investigating polymicrobial communities where DNA preservation is suboptimal. Full article
(This article belongs to the Special Issue Veterinary Microbiology and Diagnostics)
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19 pages, 4910 KiB  
Article
Investigation of Antibiotic Resistance of E. coli Associated with Farm Animal Feces with Participation of Citizen Scientists
by Anna M. Timofeeva, Maria R. Galyamova, Dmitriy M. Krivosheev, Sergey Yu. Karabanov and Sergey E. Sedykh
Microorganisms 2024, 12(11), 2308; https://doi.org/10.3390/microorganisms12112308 - 13 Nov 2024
Viewed by 1311
Abstract
This paper presents the findings of a large-scale study on antibiotic resistance in bacteria found in farm animal feces across Russia. The study included 6578 samples of farm animal manure from 13 regions in Russia, with the help of citizen scientists. Molecular and [...] Read more.
This paper presents the findings of a large-scale study on antibiotic resistance in bacteria found in farm animal feces across Russia. The study included 6578 samples of farm animal manure from 13 regions in Russia, with the help of citizen scientists. Molecular and microbiological methods were used to analyze 1111 samples of E. coli. The microbiological analysis focused on culturing the microorganisms present in the fecal samples on selective media for E. coli and evaluating the sensitivity of the bacteria to different antibiotics, including ampicillin, tetracycline, chloramphenicol, cefotaxime, and ciprofloxacin. The molecular analysis involved isolating the genomic DNA of the bacteria and conducting PCR assays to detect the vanA, vanB, and mcr-1 antibiotic resistance genes. The results demonstrated significant differences in antibiotic sensitivity of the samples that are morphologically identical to E. coli from different regions. For example, 98.0% and 82.5% of E. coli and other fecal bacterial isolates from the Omsk and Vologda regions lacked antibiotic resistance genes, while 97.7% of samples from the Voronezh region possessed three resistance genes simultaneously. The phenotypic antibiotic sensitivity test also revealed regional differences. For instance, 98.1% of fecal bacterial samples from cattle in the Udmurt Republic were sensitive to all five antibiotics tested, whereas 92.8% of samples from the Voronezh region showed resistance to all five antibiotics. The high level of antibiotic resistance observed may be attributed to their use in farming practices. The distinctive feature of our research is that comprehensive geographical coverage was achieved by using a citizen science platform. Citizen scientists, specifically students from colleges and universities, were responsible for the collection and initial analysis of samples. The project attracted 3096 student participants, enabling the collection and analysis of a significant number of samples from various locations in Russia. Full article
(This article belongs to the Special Issue Veterinary Microbiology and Diagnostics)
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Other

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10 pages, 2892 KiB  
Case Report
Successful Treatment of Fungal Dermatitis in a Bottlenose Dolphin (Tursiops truncatus)
by Takashi Kamio, Honoka Nojo, Rui Kano, Mami Murakami, Yukako Odani, Koji Kanda, Tomoko Mori, Yuichiro Akune, Masanori Kurita, Ayaka Okada and Yasuo Inoshima
Microorganisms 2025, 13(1), 106; https://doi.org/10.3390/microorganisms13010106 - 7 Jan 2025
Viewed by 1274
Abstract
In recent decades, many fungi have emerged as major causes of disease in marine mammals. This study reports on the detection of filamentous fungi in the subcutaneous tissue and wound surface on the tail fin of a managed bottlenose dolphin (Tursiops truncatus [...] Read more.
In recent decades, many fungi have emerged as major causes of disease in marine mammals. This study reports on the detection of filamentous fungi in the subcutaneous tissue and wound surface on the tail fin of a managed bottlenose dolphin (Tursiops truncatus) emaciated due to severe digestive problems. Immunosuppression by chronic diseases and starvation decreased resistance against opportunistic infections. Sequencing analysis revealed that the fungi on the wound surface were Fusarium oxysporum, and antifungal susceptibility testing was performed. In the subcutaneous tissue, dematiaceous fungi were identified using histopathological examination. Combination antifungal treatment with voriconazole and terbinafine and surgical resection were performed, in addition to daily debridement with polyaminopropyl biguanide (PHMB) and betaine. Hematological examination revealed a reduction in inflammatory markers after antifungal treatment and surgical resection of necrotic tissue on the edge of the tail fin. The co-administration of synergistic agents voriconazole and terbinafine, in conjunction with surgical debridement, successfully eliminated pheohyphomycosis and fusariomycosis in the bottlenose dolphin. Wound healing was achieved using systematic antifungals and daily debridement with PHMB and betaine. This is the first report of successful treatment of pheohyphomycosis and fusariomycosis in a bottlenose dolphin using voriconazole and terbinafine combination therapy and surgical resection. Full article
(This article belongs to the Special Issue Veterinary Microbiology and Diagnostics)
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