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Microbiology Research
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Diagnostics and Molecular Epidemiology of Infectious Diseases in Veterinary Medicine
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Diagnostics and Molecular Epidemiology of Infectious Diseases in Veterinary Medicine

A special issue of Microbiology Research (ISSN 2036-7481).

Deadline for manuscript submissions: 30 June 2026 | Viewed by 1904

Special Issue Editor

Dr. Seyed Ali Ghorashi

E-Mail Website
Guest Editor
1. School of Agricultural, Environmental and Veterinary Sciences, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
2. Gulbali Institute, Charles Sturt University, Wagga Wagga, NSW 2678, Australia
Interests: molecular diagnosis and genotyping of veterinary pathogens; epidemiology of infectious diseases
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

This Special Issue is proposed in collaboration with Microorganisms and Microbiology Research and focuses on the theme: Diagnostic innovations and molecular epidemiology of infectious diseases in Veterinary Medicine.

The aim of this Special Issue is to highlight recent advancements in the detection, molecular characterisation, and epidemiological investigation of infectious diseases affecting domestic and companion animals. As veterinary medicine continues to face complex challenges, such as the rise of zoonotic pathogens, antimicrobial resistance, and emerging infectious threats, innovative diagnostic solutions and reliable epidemiological insights are becoming increasingly critical.

The Special Issue will welcome original research articles, reviews, and short communications that contribute to the understanding, development, or application of diagnostic and molecular approaches relevant to bacterial, viral, and fungal pathogens in veterinary contexts. While particular encouragement will be given to submissions focusing on the development and evaluation of rapid, cost-effective, and field-deployable point-of-care diagnostic tests, the scope will remain broad, covering molecular typing, disease surveillance, improvements in sample processing, and other diagnostic innovations.

This initiative also aims to facilitate the exchange of knowledge among professionals and researchers working in veterinary microbiology and infectious disease diagnostics. By promoting interdisciplinary collaboration and sharing of practical and scientific advancements, the Special Issue seeks to support progress in both animal health and broader public health contexts.

You may choose our Joint Special Issue in Microorganisms.

Dr. Seyed Ali Ghorashi
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Microbiology Research is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Veterinary Medicine
  • Veterinary Science
  • Veterinary Pathology
  • Molecular Epidemiology
  • Animal Science
  • Veterinary Diagnostics
  • Molecular Diagnostics
  • Infectious Diseases
  • Point-of-Care Testing
  • Animal Pathogens
  • Zoonotic Diseases
  • Molecular Typing
  • Surveillance and Monitoring
  • Veterinary Microbiology
  • Diagnostic Assay Development
  • Rapid Diagnostics
  • Veterinary Pathogens
  • Wildlife Microbiology

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (3 papers)

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Research

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18 pages, 2860 KB  
Article
Urinalysis and Antimicrobial Susceptibility of Bacteria Isolated from Urine of Dogs and Cats in Poland in 2023: Associations Between Urine Parameters and Bacteriuria
by Dawid Jańczak, Piotr Górecki, Natalia Skrzypek, Dominika Sobkiewicz, Magda Paczocha, Aleksander Chrzanowski, Aleksandra Kornelia Maj, Rafał Stryjek, Aleksandra Anna Zasada and Anna Golke
Microbiol. Res. 2026, 17(1), 11; https://doi.org/10.3390/microbiolres17010011 - 7 Jan 2026
Viewed by 209
Abstract
Bacterial urinary tract infections (UTIs) are common in dogs and cats. This study examined the correlations between routine urinalysis and culture-confirmed infections and described the etiologic agents and antimicrobial susceptibility to support stewardship. In 2023, 1787 urine samples (854 dogs, 933 cats) underwent [...] Read more.
Bacterial urinary tract infections (UTIs) are common in dogs and cats. This study examined the correlations between routine urinalysis and culture-confirmed infections and described the etiologic agents and antimicrobial susceptibility to support stewardship. In 2023, 1787 urine samples (854 dogs, 933 cats) underwent urinalysis, aerobic culture with species identification, and disk-diffusion testing per Clinical and Laboratory Standards Institute standards; non-parametric statistics with effect sizes were applied. Pyuria was the strongest correlate of infection in both species. Low urine specific gravity was associated with infection and crystal detection, and urine pH correlated weakly with growth in dogs. Nitrite positivity was strongly associated with infection in dogs but showed no diagnostic value in cats. Hematuria showed a weak inverse association in dogs and no association in cats. Females and older animals were more frequently infected, and infections were slightly more common in summer. Most episodes were monomicrobial (85%), predominantly caused by Escherichia coli (48.4% of dogs; 51.5% of cats). E. coli remained broadly susceptible to nitrofurantoin and aminoglycosides. Fluoroquinolone activity was variable. Pseudomonas spp. showed the highest susceptibility to ceftazidime, cefepime, and aminoglycosides. These findings support culture when pyuria, dilute urine, or nitrite positivity is detected and favour short, targeted empiric therapy pending results, guided by a stepwise clinical decision pathway. Full article
(This article belongs to the Special Issue Diagnostics and Molecular Epidemiology of Infectious Diseases in Veterinary Medicine)
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23 pages, 2707 KB  
Article
Sentinel or Disperser? The Role of White Storks (Ciconia ciconia) in the Spread of Antibiotic-Resistant Bacteria
by Teresa Cardona-Cabrera, Sandra Martínez-Álvarez, Yolanda Muela-Trujillo, Alberto Sánchez-Cano, Juan Carlos Montero, Juan Manuel Fernández-Gallego, Carmen Torres and Ursula Höfle
Microbiol. Res. 2025, 16(9), 202; https://doi.org/10.3390/microbiolres16090202 - 6 Sep 2025
Viewed by 942
Abstract
Antimicrobial resistance (AMR) represents a threat to human, animal, and environmental health. This study evaluated the potential role of birds as AMR dispersers in white storks as a model species investigating dispersal between locations connected by their movements. Throughout a year, 346 samples [...] Read more.
Antimicrobial resistance (AMR) represents a threat to human, animal, and environmental health. This study evaluated the potential role of birds as AMR dispersers in white storks as a model species investigating dispersal between locations connected by their movements. Throughout a year, 346 samples were collected from two landfills (225 fecal samples) and two wetlands (93 fecal/28 water samples) based on satellite tracking data that confirmed continuous stork movements between foraging (landfill) and nighttime roosting (wetlands) locations. Samples were seeded in selective media with antibiotics for the isolation of antibiotic-resistant bacteria. A total of 313 isolates from 35 different bacterial species were obtained, with an AMR prevalence of 43.1% in fecal samples from landfills, 7.5% in fecal samples from wetlands, and 21.4% in water from wetlands. Multidrug resistant bacteria were only found in fecal samples (19.6% landfills/4.3% wetlands) and extended-spectrum beta-lactamase producing-bacteria were found exclusively in fecal samples from landfills with a marked presence during the fall migration period. Our study reveals marked differences in AMR prevalence and resistance phenotypes between study locations and fecal and water samples throughout the year, thus not supporting a clear role of storks as AMR dispersers. Nevertheless, similar changes in AMR phenotype prevalences during fall migration in stork faecal samples from one of the tested landfills and its paired wetland, and the significant increase in ESBL-producing Enterobacterales prevalence matching the arrival of migratory white storks in fall, underline the need for more in-depth genome-based studies to elucidate the role of white storks as dispersers or sentinels of AMR. Full article
(This article belongs to the Special Issue Diagnostics and Molecular Epidemiology of Infectious Diseases in Veterinary Medicine)
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Review

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32 pages, 442 KB  
Review
Bacterial Bovine Respiratory Disease: A Comprehensive Review of Etiology, Pathogenesis and Management Strategies
by Chiara Storoni, Silvia Preziuso, Anna-Rita Attili, Yubao Li and Vincenzo Cuteri
Microbiol. Res. 2026, 17(1), 18; https://doi.org/10.3390/microbiolres17010018 - 11 Jan 2026
Viewed by 170
Abstract
Bovine Respiratory Disease (BRD) represents one of the largest causes of economic loss and animal morbidity in the global cattle industry, second only to neonatal diarrhea. Its etiology is complex, originating from a multifactorial combination of host susceptibility, environmental stressors, viral infections, and [...] Read more.
Bovine Respiratory Disease (BRD) represents one of the largest causes of economic loss and animal morbidity in the global cattle industry, second only to neonatal diarrhea. Its etiology is complex, originating from a multifactorial combination of host susceptibility, environmental stressors, viral infections, and secondary bacterial pathogens. Although viruses are often the initial cause of disease, suppressing the host’s respiratory defense mechanisms, most of the severe pneumonic damage and clinical signs can be attributed to bacterial infections. This review provides an overview of the primary bacterial agents identified within the BRD complex, including Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, and Mycoplasma bovis. We discuss their role as commensals that then become opportunistic pathogens, and further how they interact in a synergistic relationship with a primary viral insult, leading to the resulting pathogenesis and the development of pneumonia. This manuscript discusses in further detail some of the challenges in BRD management, such as the limitations of current diagnostic methodologies, overreliance on antimicrobial therapy, and the growing concern of antimicrobial resistance. Lastly, the need for integrated approaches in management, better husbandry and biosecurity, coupled with the development of novel therapeutic alternatives, is underlined as a means of assuring a sustainable control of this serious syndrome. Full article
(This article belongs to the Special Issue Diagnostics and Molecular Epidemiology of Infectious Diseases in Veterinary Medicine)
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