DNA-Based Electrochemical Biosensors and Aptasensors: Advances in Medical Diagnostics and Detection

A special issue of Micromachines (ISSN 2072-666X). This special issue belongs to the section "B1: Biosensors".

Deadline for manuscript submissions: 28 February 2026 | Viewed by 636

Special Issue Editor


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Guest Editor
Department of Biology, Western Kentucky University, Bowling Green, KY 42101, USA
Interests: aptamer; aptasensor; lateral flow cartridges; wearable biosensors; emerging biosensors; POC biosensors; nanobiosensors; DNA chips; microarray; gene expression; cancer cell therapeutics
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Special Issue Information

Dear Colleagues,

This Special Issue, "DNA-Based Electrochemical Biosensors and Aptasensors: Advances in Medical Diagnostics and Detection", aims to explore the latest advancements in biosensor technologies tailored for medical diagnostics. Electrochemical DNA biosensors and aptasensors represent a rapidly growing field, offering unique advantages such as high sensitivity, specificity, rapid response, and potential for miniaturization. These sensors are pivotal in addressing the increasing demand for accurate and reliable diagnostic tools in healthcare.

This Special Issue will serve as a platform for researchers to showcase novel approaches and breakthroughs in sensor development and their applications in detecting biomarkers, pathogens, and disease-related molecules. We encourage submissions that focus on innovations in design, material integration, and analytical performance, as well as clinical and real-world applications. Topics of interest include, but are not limited to, the following:

  • The development of new electrochemical biosensor and aptasensor platforms.
  • Advanced materials and nanotechnologies for enhanced aptasensor performance.
  • Applications in point-of-care diagnostics, personalized medicine, and the rapid detection of diseases.
  • Integration of biosensors with microfluidic systems and wearable devices.
  • Multiplexed detection and its implications for simultaneous analysis.
  • Case studies demonstrating the clinical validation of biosensors and aptasensors for medical applications.

By bringing together experts from diverse fields such as biosensing, nanotechnology, biochemistry, and clinical diagnostics, this Special Issue aims to advance the understanding and development of biosensor technologies that can revolutionize medical diagnostics and improve healthcare outcomes globally.

We look forward to receiving your submissions!

Prof. Dr. Van Thuan Nguyen
Guest Editor

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Keywords

  • electrochemical biosensors
  • aptamer-based biosensor
  • aptasensors
  • medical diagnostics
  • point-of-care testing
  • biomarker detection
  • pathogen detection
  • DNA/RNA biosensors
  • nanotechnology-based sensors
  • biomedical applications
  • microfluidic integration
  • clinical diagnostics
  • rapid detection technologies
  • electrochemical detection methods

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Published Papers (1 paper)

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Research

19 pages, 3561 KB  
Article
Pump-Free Insulin Delivery via an SLA-Printed Hollow Microneedle Patch with an Integrated Self-Sealing Reservoir
by Evie Smith, Naser A. Alsaleh, Mahmoud Ahmadein, Abdullah A. Elfar, Hany Hassanin and Khamis Essa
Micromachines 2025, 16(12), 1322; https://doi.org/10.3390/mi16121322 - 26 Nov 2025
Viewed by 427
Abstract
Hollow microneedle (HMN) systems can deliver insulin with minimal pain, but most rely on external pumps that add bulk, cost, and failure modes. This paper reports the design, fabrication, and mechanical characterisation of a pump-free, refillable HMN patch that integrates a syringe-loadable, self-sealing [...] Read more.
Hollow microneedle (HMN) systems can deliver insulin with minimal pain, but most rely on external pumps that add bulk, cost, and failure modes. This paper reports the design, fabrication, and mechanical characterisation of a pump-free, refillable HMN patch that integrates a syringe-loadable, self-sealing reservoir and delivers by passive diffusion. A 3 × 4 array of side-orifice conical HMNs with a target height of 1 mm and a bore of 0.8 mm was stereolithography-printed in dental-grade resin and coupled to an elastic-grade resin septum that maintains a leak-free seal after repeated needle puncture. A surface-response design of experiments (DoE) probed wall thickness of 0.10–0.20 mm, post-cure time of 20–60 min, and temperatures of 35–80 °C. The microneedle characteristics include geometric fidelity, insertion into multilayer Parafilm, and axial compression to 150 N. All patches were printed with a hollow channel and side orifices with tips were slightly blunted. Relative to the original design, height undershoot was from −24.5% to −60.5% while base diameters were within −11% to +20%. Parafilm insertion exhibited a peak then force drop at about 0.22 mm displacement with 1.2–1.5 N pierced the first layer. It was found that about 90% of needles penetrated about 381 µm and more than 20% reached 635 µm. Patches withstood 150 N without fracture with strains of 9.7–15.6% and modulus of 8–48 MPa. ANOVA identified wall thickness as a significant factor, with curing temperature not being significant. Contour analysis defined an operating window near a 0.15 mm wall and about 40 min post-cure balancing dimensional fidelity and post-compression height retention. These results define a manufacturable path to compact, pump-free insulin patches with low insertion force and robust mechanics, opening a clinically scalable route to simpler everyday insulin therapy. Full article
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