Special Issue "Recent Advances in Periodontics and Dental Implantology: Part II"

A special issue of Medicina (ISSN 1648-9144). This special issue belongs to the section "Dentistry".

Deadline for manuscript submissions: 31 January 2024 | Viewed by 619

Special Issue Editor

Department of Periodontics, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
Interests: bone; bone biology; tissue engineering; stem cells; instrumentation; enzyme kinetics; bone regeneration; dental implants; biomaterials; oral health
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Special Issue Information

Dear Colleagues,

With the advancement of technology, great progress has been made in the periodontology field. The prevalence of periodontal disease is relatively high and has been reported at 20­–45% or higher. Various treatments and biomaterials have been applied to increase the effectiveness of periodontal treatment. The long-term effects of periodontal treatment have been published. Despite advances in periodontal treatment, tooth extraction and further implant placement are being made. A protocol to increase the success rate of implants has been proposed. Various methods have been proposed that can be used in areas where the placement of dental implants is difficult. Stem cells and growth factors have been used as various biomaterials.

The scope of this Special Issue will serve as a forum for papers addressing the following concepts:

  • Understanding and mechanisms of periodontal disease;
  • Treatment of periodontal disease;
  • Short- and long-term effects of periodontal treatment;
  • Various soft and hard tissue regeneration methods;
  • Clinical outcome of dental implants;
  • Enhancement of efficacy with application of growth factors;
  • Cell therapy in periodontal and implant treatment.

Dr. Jun-Beom Park
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Medicina is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1800 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • periodontitis
  • inflammation
  • epidemiology
  • oral health
  • bone regeneration
  • bone biology
  • tissue engineering
  • dental implants
  • biomaterials
  • stem cells

Published Papers (1 paper)

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Research

Article
Bone Morphogenetic Protein-9 Promotes Osteogenic Differentiation and Mineralization in Human Stem-Cell-Derived Spheroids
Medicina 2023, 59(7), 1315; https://doi.org/10.3390/medicina59071315 - 16 Jul 2023
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Abstract
Background and Objectives: Alkaline phosphatase activity, mineralized matrix, and osteogenic-related gene expression have been shown to increase in response to bone morphogenetic protein-9 (BMP-9). In this study, spheroids derived from human gingival stem cells were used to determine the effects of BMP-9 on [...] Read more.
Background and Objectives: Alkaline phosphatase activity, mineralized matrix, and osteogenic-related gene expression have been shown to increase in response to bone morphogenetic protein-9 (BMP-9). In this study, spheroids derived from human gingival stem cells were used to determine the effects of BMP-9 on cell survival, osteogenesis, and mineralization. Materials and Methods: Human gingival stem cells were used to produce spheroids and then grown to concentrations of 0, 0.1, 1, 10, and 100 ng/mL with BMP-9. On days 1, 3, 5, and 7, morphological examination was carried out. A live/dead assay and Cell Counting Kit-8 was used to assess the vitality of cells. On days 7 and 14, alkaline phosphatase activity assays were carried out using a commercially available kit to examine the osteogenic differentiation of cell spheroids. Alizarin Red Staining was performed on the 7th and 14th days to evaluate mineralization, and RUNX2 and COL1A1 expression levels were evaluated on the 7th and 14th days using real-time polymerase chain reactions. Results: The BMP-9 added at the measured quantities did not appear to alter the shape of the well-formed spheroids produced by stem cells on day 1. In addition, treatment with BMP-9 at doses of 0, 0.1, 1, 10, or 100 ng/mL did not significantly alter cell diameter. Throughout the whole experimental process, viability was maintained. On day 14, the alkaline phosphatase activity in the groups dosed with 0.1, 1, 10, or 100 ng/mL was statistically higher than that in the unloaded control group (p < 0.05). According to qPCR data, the mRNA expression level of RUNX2 with 1 ng/mL dosing was higher on day 7 compared to that of the unloaded control group (p < 0.05). Conclusions: These findings suggest that BMP-9 can be employed to stimulate early osteogenic differentiation in stem cell spheroids. Full article
(This article belongs to the Special Issue Recent Advances in Periodontics and Dental Implantology: Part II)
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