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Marine Drugs
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3rd International Conference of the Marine Fungal Natural Products (MaFNaP)
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3rd International Conference of the Marine Fungal Natural Products (MaFNaP)

A special issue of Marine Drugs (ISSN 1660-3397).

Deadline for manuscript submissions: closed (31 December 2021) | Viewed by 16520

Special Issue Editor

Dr. Mohamed MEHIRI

E-Mail Website
Guest Editor
Université Côte d’Azur, Nice, France
Interests: Marine Natural Products Chemistry; Isolation; Structure Elucidation; Synthesis; Macroorganisms; Microorganisms; Marine Fungi; Metabolomics; Bioactivity
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Fungi constitute an important proportion of marine microbiota. Marine fungi are a renewable and outstanding source for new bioactive natural products that can contribute significantly to the well-being of the society. However, their potential in many areas has largely remained untapped.

The Marine Natural Products Team, which is part of the Institute of Chemistry of Nice at the University Côte d’Azur (France), cordially invites you to the 3rd International Conference of the Marine Fungal Natural Products (MaFNaP) that will be held in Nice (France) from 15-17 June 2020. This event will gather an international audience to discuss new developments and approaches within the field, enhance awareness on marine fungi as a sustainable resource for blue biotechnology, and highlight the potential of marine fungal metabolites in various areas. It also serves as a forum for networking and the promotion of young scientists working in the field.

The initiative for a systematic work on marine fungi and MaFNaPs stems from the meeting of scientists in Prince Edward Island, Canada, that was followed by the official establishment of the MaFNaP Consortium in 2014. The very first MaFNaP conference was held in 2015 and hosted by the Faculty of Pharmacy of the University of Nantes (France). The first Marine Drugs Special Issue, comprising selected papers from this conference, are available at https://www.mdpi.com/journal/marinedrugs/special_issues/fungal-consortium. The 2nd MaFNaP_2017 conference in Kiel (Germany) has stimulated scientific interactions, networking, and collaborations for innovative research to reveal the real capacity of marine fungi. The second Marine Drugs Special Issue, comprising selected papers from this conference, are available at https://www.mdpi.com/journal/marinedrugs/special_issues/fungal-consortium-2017.

MaFNaP conferences are dedicated to bringing the marine fungal research community together. On behalf of the MaFNaP consortium and the scientific committee of the MaFNaP_2020 conference, we kindly invite submissions in these topics, plus any topics related to marine fungal natural products.

Dr. Mohamed MEHIRI
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Marine Drugs is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • Marine Fungal natural products
  • Marine fungal biodiscovery
  • Marine fungal biotechnology
  • Deep sea
  • Co-culture
  • Metabolomics
  • Synthesis

Published Papers (5 papers)

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Research

21 pages, 2742 KiB  
Article
New Phenylspirodrimanes from the Sponge-Associated Fungus Stachybotrys chartarum MUT 3308
by Marie Dayras, Estelle Sfecci, Elena Bovio, Olivia Rastoin, Maeva Dufies, Fabien Fontaine-Vive, Elisabeth Taffin-de-Givenchy, Thierry Lacour, Gilles Pages, Giovanna Cristina Varese and Mohamed Mehiri
Mar. Drugs 2023, 21(3), 135; https://doi.org/10.3390/md21030135 - 21 Feb 2023
Cited by 3 | Viewed by 2760
Abstract
Two phenylspirodrimanes, never isolated before, stachybotrin J (1) and new stachybocin G (epi-stachybocin A) (2), along with the already reported stachybotrin I (3), stachybotrin H (4), stachybotrylactam (5), stachybotrylactam acetate ( [...] Read more.
Two phenylspirodrimanes, never isolated before, stachybotrin J (1) and new stachybocin G (epi-stachybocin A) (2), along with the already reported stachybotrin I (3), stachybotrin H (4), stachybotrylactam (5), stachybotrylactam acetate (6), 2α-acetoxystachybotrylactam acetate (7), stachybotramide (8), chartarlactam B (9), and F1839-J (10) were isolated from the sponge-associated fungus Stachybotrys chartarum MUT 3308. Their structures were established based on extensive spectrometric (HRMS) and spectroscopic (1D and 2D NMR) analyses. Absolute configurations of the stereogenic centers of stachybotrin J (1), stachybocin G (2), and stachybotrin I (3), were determined by comparison of their experimental circular dichroism (CD) spectra with their time-dependent density functional theory (TD-DFT) circular dichroism (ECD) spectra. The putative structures of seventeen additional phenylspirodrimanes were proposed by analysis of their respective MS/MS spectra through a Feature-Based Molecular Networking approach. All the isolated compounds were evaluated for their cytotoxicity against five aggressive cancer cell lines (MP41, 786, 786R, CAL33, and CAL33RR), notably including two resistant human cancer cell lines (786R, CAL33RR), and compounds 5, 6, and 7 exhibited cytotoxicity with IC50 values in the range of 0.3−2.2 µM. Full article
(This article belongs to the Special Issue 3rd International Conference of the Marine Fungal Natural Products (MaFNaP))
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15 pages, 3388 KiB  
Article
Highlighting the Biotechnological Potential of Deep Oceanic Crust Fungi through the Prism of Their Antimicrobial Activity
by Maxence Quemener, Marie Dayras, Nicolas Frotté, Stella Debaets, Christophe Le Meur, Georges Barbier, Virginia Edgcomb, Mohamed Mehiri and Gaëtan Burgaud
Mar. Drugs 2021, 19(8), 411; https://doi.org/10.3390/md19080411 - 24 Jul 2021
Cited by 5 | Viewed by 2309
Abstract
Among the different tools to address the antibiotic resistance crisis, bioprospecting in complex uncharted habitats to detect novel microorganisms putatively producing original antimicrobial compounds can definitely increase the current therapeutic arsenal of antibiotics. Fungi from numerous habitats have been widely screened for their [...] Read more.
Among the different tools to address the antibiotic resistance crisis, bioprospecting in complex uncharted habitats to detect novel microorganisms putatively producing original antimicrobial compounds can definitely increase the current therapeutic arsenal of antibiotics. Fungi from numerous habitats have been widely screened for their ability to express specific biosynthetic gene clusters (BGCs) involved in the synthesis of antimicrobial compounds. Here, a collection of unique 75 deep oceanic crust fungi was screened to evaluate their biotechnological potential through the prism of their antimicrobial activity using a polyphasic approach. After a first genetic screening to detect specific BGCs, a second step consisted of an antimicrobial screening that tested the most promising isolates against 11 microbial targets. Here, 12 fungal isolates showed at least one antibacterial and/or antifungal activity (static or lytic) against human pathogens. This analysis also revealed that Staphylococcus aureus ATCC 25923 and Enterococcus faecalis CIP A 186 were the most impacted, followed by Pseudomonas aeruginosa ATCC 27853. A specific focus on three fungal isolates allowed us to detect interesting activity of crude extracts against multidrug-resistant Staphylococcus aureus. Finally, complementary mass spectrometry (MS)-based molecular networking analyses were performed to putatively assign the fungal metabolites and raise hypotheses to link them to the observed antimicrobial activities. Full article
(This article belongs to the Special Issue 3rd International Conference of the Marine Fungal Natural Products (MaFNaP))
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26 pages, 4016 KiB  
Article
Untargeted Metabolomics Approach for the Discovery of Environment-Related Pyran-2-Ones Chemodiversity in a Marine-Sourced Penicillium restrictum
by Van-Tuyen Le, Samuel Bertrand, Thibaut Robiou du Pont, Fabrice Fleury, Nathalie Caroff, Sandra Bourgeade-Delmas, Emmanuel Gentil, Cedric Logé, Gregory Genta-Jouve and Olivier Grovel
Mar. Drugs 2021, 19(7), 378; https://doi.org/10.3390/md19070378 - 29 Jun 2021
Cited by 6 | Viewed by 3811
Abstract
Very little is known about chemical interactions between fungi and their mollusc host within marine environments. Here, we investigated the metabolome of a Penicillium restrictum MMS417 strain isolated from the blue mussel Mytilus edulis collected on the Loire estuary, France. Following the OSMAC [...] Read more.
Very little is known about chemical interactions between fungi and their mollusc host within marine environments. Here, we investigated the metabolome of a Penicillium restrictum MMS417 strain isolated from the blue mussel Mytilus edulis collected on the Loire estuary, France. Following the OSMAC approach with the use of 14 culture media, the effect of salinity and of a mussel-derived medium on the metabolic expression were analysed using HPLC-UV/DAD-HRMS/MS. An untargeted metabolomics study was performed using principal component analysis (PCA), orthogonal projection to latent structure discriminant analysis (O-PLSDA) and molecular networking (MN). It highlighted some compounds belonging to sterols, macrolides and pyran-2-ones, which were specifically induced in marine conditions. In particular, a high chemical diversity of pyran-2-ones was found to be related to the presence of mussel extract in the culture medium. Mass spectrometry (MS)- and UV-guided purification resulted in the isolation of five new natural fungal pyran-2-one derivatives—5,6-dihydro-6S-hydroxymethyl-4-methoxy-2H-pyran-2-one (1), (6S, 1’R, 2’S)-LL-P880β (3), 5,6-dihydro-4-methoxy-6S-(1’S, 2’S-dihydroxy pent-3’(E)-enyl)-2H-pyran-2-one (4), 4-methoxy-6-(1’R, 2’S-dihydroxy pent-3’(E)-enyl)-2H-pyran-2-one (6) and 4-methoxy-2H-pyran-2-one (7)—together with the known (6S, 1’S, 2’S)-LL-P880β (2), (1’R, 2’S)-LL-P880γ (5), 5,6-dihydro-4-methoxy-2H-pyran-2-one (8), (6S, 1’S, 2’R)-LL-P880β (9), (6S, 1’S)-pestalotin (10), 1’R-dehydropestalotin (11) and 6-pentyl-4-methoxy-2H-pyran-2-one (12) from the mussel-derived culture medium extract. The structures of 1-12 were determined by 1D- and 2D-MMR experiments as well as high-resolution tandem MS, ECD and DP4 calculations. Some of these compounds were evaluated for their cytotoxic, antibacterial, antileishmanial and in-silico PTP1B inhibitory activities. These results illustrate the utility in using host-derived media for the discovery of new natural products. Full article
(This article belongs to the Special Issue 3rd International Conference of the Marine Fungal Natural Products (MaFNaP))
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21 pages, 2240 KiB  
Article
Identification and Characterization of a New Type III Polyketide Synthase from a Marine Yeast, Naganishia uzbekistanensis
by Laure Martinelli, Vanessa Redou, Bastien Cochereau, Ludovic Delage, Nolwenn Hymery, Elisabeth Poirier, Christophe Le Meur, Gaetan Le Foch, Lionel Cladiere, Mohamed Mehiri, Nathalie Demont-Caulet and Laurence Meslet-Cladiere
Mar. Drugs 2020, 18(12), 637; https://doi.org/10.3390/md18120637 - 11 Dec 2020
Cited by 4 | Viewed by 2988
Abstract
A putative Type III Polyketide synthase (PKSIII) encoding gene was identified from a marine yeast, Naganishia uzbekistanensis strain Mo29 (UBOCC-A-208024) (formerly named as Cryptococcus sp.) isolated from deep-sea hydrothermal vents. This gene is part of a distinct phylogenetic branch compared to all known [...] Read more.
A putative Type III Polyketide synthase (PKSIII) encoding gene was identified from a marine yeast, Naganishia uzbekistanensis strain Mo29 (UBOCC-A-208024) (formerly named as Cryptococcus sp.) isolated from deep-sea hydrothermal vents. This gene is part of a distinct phylogenetic branch compared to all known terrestrial fungal sequences. This new gene encodes a C-terminus extension of 74 amino acids compared to other known PKSIII proteins like Neurospora crassa. Full-length and reduced versions of this PKSIII were successfully cloned and overexpressed in a bacterial host, Escherichia coli BL21 (DE3). Both proteins showed the same activity, suggesting that additional amino acid residues at the C-terminus are probably not required for biochemical functions. We demonstrated by LC-ESI-MS/MS that these two recombinant PKSIII proteins could only produce tri- and tetraketide pyrones and alkylresorcinols using only long fatty acid chain from C8 to C16 acyl-CoAs as starter units, in presence of malonyl-CoA. In addition, we showed that some of these molecules exhibit cytotoxic activities against several cancer cell lines. Full article
(This article belongs to the Special Issue 3rd International Conference of the Marine Fungal Natural Products (MaFNaP))
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12 pages, 929 KiB  
Article
Pyrenosetin D, a New Pentacyclic Decalinoyltetramic Acid Derivative from the Algicolous Fungus Pyrenochaetopsis sp. FVE-087
by Bicheng Fan, Pradeep Dewapriya, Fengjie Li, Laura Grauso, Martina Blümel, Alfonso Mangoni and Deniz Tasdemir
Mar. Drugs 2020, 18(6), 281; https://doi.org/10.3390/md18060281 - 26 May 2020
Cited by 16 | Viewed by 3379
Abstract
The fungal genus Pyrenochaetopsis is commonly found in soil, terrestrial, and marine environments, however, has received little attention as a source of bioactive secondary metabolites so far. In a recent work, we reported the isolation and characterization of three new anticancer decalinoyltetramic acid [...] Read more.
The fungal genus Pyrenochaetopsis is commonly found in soil, terrestrial, and marine environments, however, has received little attention as a source of bioactive secondary metabolites so far. In a recent work, we reported the isolation and characterization of three new anticancer decalinoyltetramic acid derivatives, pyrenosetins A–C, from the Baltic Fucus vesiculosus-derived endophytic fungus Pyrenochaetopsis sp. FVE-001. Herein we report a new pentacyclic decalinoylspirotetramic acid derivative, pyrenosetin D (1), along with two known decalin derivatives wakodecalines A (2) and B (3) from another endophytic strain Pyrenochaetopsis FVE-087 isolated from the same seaweed and showed anticancer activity in initial screenings. The chemical structures of the purified compounds were elucidated by comprehensive analysis of HR-ESIMS, FT-IR, [α]D, 1D and 2D NMR data coupled with DFT calculations of NMR parameters and optical rotation. Compounds 1–3 were evaluated for their anticancer and toxic potentials against the human malignant melanoma cell line (A-375) and the non-cancerous keratinocyte cell line (HaCaT). Pyrenosetin D (1) showed toxicity towards both A-375 and HaCaT cells with IC50 values of 77.5 and 39.3 μM, respectively, while 2 and 3 were inactive. This is the third chemical study performed on the fungal genus Pyrenochaetopsis and the first report of a pentacyclic decalin ring system from the fungal genus Pyrenochaetopsis. Full article
(This article belongs to the Special Issue 3rd International Conference of the Marine Fungal Natural Products (MaFNaP))
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