Marine Functional Carbohydrates: Enzymatic Manufacturing and Activity Evaluation

A special issue of Marine Drugs (ISSN 1660-3397).

Deadline for manuscript submissions: 31 July 2024 | Viewed by 432

Special Issue Editor


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Guest Editor
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, China
Interests: enzymes in glycan modification; functional glycan biosynthesis; bioactivity evaluation; probiotics excavation and engineering

Special Issue Information

Dear Colleagues,

Marine functional carbohydrates present vast opportunities for nutraceutical, medicine, and food discovery. In order to fully utilize and exploit these resources, biological tools including enzymes and microorganisms can be employed to exert effective and efficient impacts on carbohydrate acquisition and glycan processing. Recent advances in DNA sequencing and high-thoughput screening have led to significant developments in the exploration of novel efficient biocatalysts. In addition, omics research such as genomics and metabolomics also promote activity evaluation studies at a deeper level using cell and animal models.

For this Special Issue, we invite original research articles and reviews that highlight both the biocatalysts used for the manufacturing of marine functional carbohydrates and the biological activity assessment of known or novel marine carbohydrates.

Dr. Heng Li
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Marine Drugs is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • enzyme characterization
  • enzyme expression and modification
  • microorganism isolation and optimization
  • carbohydrate manufacturing with enzyme/microorganisms
  • marine polysaccharides and oligosaccharides
  • activity evaluation
  • cell and animal model
  • impact on microflora or specific microorganisms
  • omics research
  • mechanism analysis

Published Papers (1 paper)

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Research

16 pages, 5380 KiB  
Article
High-Level Extracellular Production of a Trisaccharide-Producing Alginate Lyase AlyC7 in Escherichia coli and Its Agricultural Application
by Xiao-Han Wang, Yu-Qiang Zhang, Xin-Ru Zhang, Xiao-Dong Zhang, Xiao-Meng Sun, Xiao-Fei Wang, Xiao-Hui Sun, Xiao-Yan Song, Yu-Zhong Zhang, Ning Wang, Xiu-Lan Chen and Fei Xu
Mar. Drugs 2024, 22(5), 230; https://doi.org/10.3390/md22050230 - 18 May 2024
Viewed by 328
Abstract
Alginate oligosaccharides (AOS), products of alginate degradation by endotype alginate lyases, possess favorable biological activities and have broad applications. Although many have been reported, alginate lyases with homogeneous AOS products and secretory production by an engineered host are scarce. Herein, the alginate lyase [...] Read more.
Alginate oligosaccharides (AOS), products of alginate degradation by endotype alginate lyases, possess favorable biological activities and have broad applications. Although many have been reported, alginate lyases with homogeneous AOS products and secretory production by an engineered host are scarce. Herein, the alginate lyase AlyC7 from Vibrio sp. C42 was characterized as a trisaccharide-producing lyase exhibiting high activity and broad substrate specificity. With PelB as the signal peptide and 500 mM glycine as the additive, the extracellular production of AlyC7 in Escherichia coli reached 1122.8 U/mL after 27 h cultivation in Luria-Bertani medium. The yield of trisaccharides from sodium alginate degradation by the produced AlyC7 reached 758.6 mg/g, with a purity of 85.1%. The prepared AOS at 20 μg/mL increased the root length of lettuce, tomato, wheat, and maize by 27.5%, 25.7%, 9.7%, and 11.1%, respectively. This study establishes a robust foundation for the industrial and agricultural applications of AlyC7. Full article
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