Third-Generation Sequencing: Recent Advances and Applications in the Era of Genomics, Transcriptomics and Epitranscriptomics
A special issue of Life (ISSN 2075-1729). This special issue belongs to the section "Genetics and Genomics".
Deadline for manuscript submissions: closed (10 December 2021) | Viewed by 33038
Special Issue Editor
Interests: biochemistry; molecular biology; genomics
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
The last few years, third generation sequencing (TGS), also known as long-read sequencing, has caused a new revolution in the scientific field of genomics as it provides a way to study genomes, transcriptomes, and metagenomes at an unprecedented resolution. While NGS methods lead to the production of short reads (approximately 600 bp), long-read sequencing enables the generation of reads of up to several tens of kilobases or even up to 1 Mb, thus addressing serious limitations of previous technologies, including de novo genome assembly, structural variation analysis and haplotype phasing. Besides the newly introduced capability of real-time sequencing analysis as well as the significantly increased throughput, TGS also exhibits decreased sequencing costs, run times and error rates. In addition, TGS requires minimal input material, thus eliminating biases inherent to previous sequencing technologies, such as the PCR amplification step that was needed during the NGS library preparation. This enormous technological progress has not only led to the superior investigation of genomes and transcriptomes, but also has offered new capabilities beyond nucleic acid sequencing. Notably, TGS approaches have allowed the identification of specific DNA and RNA modifications, highlighting their key roles in pre-mRNA splicing, nuclear export, mRNA stability and localization, and translation efficiency. In any case, we are only witnessing the beginning of the third-generation sequencing era, and the coming years promise to bring significant developments, discoveries, and breakthroughs. The present Special Issue entitled “Third-Generation sequencing: Recent advances and applications in the era of genomics, transcriptomics and epitranscriptomics” may provide a thorough overview of the scientific progress that TGS has achieved in the last few years.
Dr. Panagiotis G. Adamopoulos
Guest Editor
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Keywords
- massive parallel sequencing
- single-molecule DNA/RNA sequencing
- post-transcriptional modifications
- genome research
- non-coding RNAs
- gene expression and regulation
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