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Life
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Recent Advances in Bone Biology
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Recent Advances in Bone Biology

A special issue of Life (ISSN 2075-1729). This special issue belongs to the section "Physiology and Pathology".

Deadline for manuscript submissions: 30 July 2026 | Viewed by 2957

Special Issue Editors

Dr. Barbara Peruzzi

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Guest Editor
Department of Life Science and Public Health, Histology and Embryology Division, Catholic University of the Sacred Heart, 00168 Rome, Italy
Interests: bone metabolism and bone cancers; osteoblast differentiation and functions; mechanotransduction and mechanobiology; aging; tissue regeneration and tissue bioengineering
Dr. Martina Leopizzi

E-Mail Website
Guest Editor
Department of Medico-Surgical Sciences and Biotechnology, Sapienza University of Rome, 00185 Rome, Italy
Interests: rare bone diseases; bone metabolism; bone turnover; bone signaling
Dr. Giulia Battafarano

E-Mail Website
Guest Editor
Bone Physiopathology Research Unit, Translational Pediatrics and Clinical Genetics Research Division, Bambino Gesù Children’s Hospital, IRCCS, 00165 Rome, Italy
Interests: bone cancer; bone metabolism; bone turnover; bone cell pathophysiology; bone diseases in children
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Bone is a mineralized and viscous-elastic connective tissue that exerts several crucial functions in the human body, such as allowing locomotion, protecting internal organs, storing and releasing fat, minerals, and hormones, producing blood cells, and providing mechanical support. Bone homeostasis is maintained through the finely tuned and regulated activities of bone cells, including osteogenic precursors, bone-forming osteoblasts, bone-resorbing osteoclasts, and mechanosensing osteocytes. Thanks to recently developed techniques in molecular and cellular biology, a deeper knowledge of bone cell physiology and pathophysiology has been achieved, allowing researchers and clinicians to better understand the mechanisms regulating bone homeostasis and to tackle bone diseases through innovative approaches. In this context, our aim for this Special Issue is to provide a comprehensive overview of recent theoretical, technical, and methodological advances in the field of bone biology. In particular, scientific contributions covering topics such as bone molecular and cellular biology, bone tissue development and repair, bone cell cross-talk, bone metabolism, bone signaling, bone cancer immunotherapy, bone-targeted therapies, the development of bone organoids for tissue engineering, and the mechanobiology of bone cells in in vitro and in vivo experimentations are welcome.

Dr. Barbara Peruzzi
Dr. Martina Leopizzi
Dr. Giulia Battafarano
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Life is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • bone tissue engineering
  • bone-targeted therapies
  • genetic bone diseases
  • bone mechanotransduction
  • bone cell pathophysiology
  • bone metabolism

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Published Papers (2 papers)

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Research

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23 pages, 8980 KB  
Article
Age-Related Alterations in SIBLING Proteins and Dentin Micro-Architecture: Morphological and Molecular Associations
by Neshka Manchorova-Veleva, Mina Pencheva, David Baruh, Veselina Todorova, Lyubomir Vangelov, Margarita Guenova, Zhelyazko Damyanov and Donka Keskinova
Life 2025, 15(12), 1919; https://doi.org/10.3390/life15121919 - 15 Dec 2025
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Abstract
Background: Aging is associated with progressive structural and functional changes in dentin, reducing its mechanical integrity and increasing vulnerability to damage. Among the most important regulators of dentin physiology are extracellular matrix proteins from the SIBLING family, including Dentin Matrix Protein 1 [...] Read more.
Background: Aging is associated with progressive structural and functional changes in dentin, reducing its mechanical integrity and increasing vulnerability to damage. Among the most important regulators of dentin physiology are extracellular matrix proteins from the SIBLING family, including Dentin Matrix Protein 1 (DMP-1), Dentin Sialophosphoprotein (DSPP), and Osteopontin (OPN). These proteins are essential for dentin mineralization, collagen organization, and tissue remodeling. Despite their critical role, knowledge about their age-related distribution and correlation with dentin structure and morphology remains limited. Aim: To examine age-dependent changes in the expression of SIBLING proteins (DMP-1, DSPP, OPN) in human dentin and to evaluate their relationship with collagen structure and ultramorphology using polarized light microscopy (PLM), immunohistochemistry (IHC), and scanning electron microscopy (SEM). Materials and Methods: Ninety extracted human teeth were categorized into young (≤17 years), mature (18–50 years), and old (>51 years) groups. IHC was applied to detect protein distribution, PLM to assess collagen organization, and SEM to evaluate dentinal morphology. Results and Conclusions: Aging was associated with increased expression of DMP-1 and OPN and a reduction in DSPP, which is particularly evident in peritubular dentin. Older samples showed collagen disorganization, reduced birefringence, and extensive intratubular mineralization. These findings suggest that age-related alterations in SIBLING proteins contribute to structural changes in dentin, providing new insights relevant to dental care in elderly patients. Full article
(This article belongs to the Special Issue Recent Advances in Bone Biology)
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11 pages, 1882 KB  
Protocol
Isolation of Human Osteal Macrophages
by Juliana Franziska Bousch, Stefanie Lichtenberg, Matthis Schnitker, Jenny Schlösser, Christoph Viktor Suschek, Uwe Maus and Christoph Beyersdorf
Life 2026, 16(3), 376; https://doi.org/10.3390/life16030376 - 27 Feb 2026
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Abstract
Osteal macrophages (“osteomacs”) are resident bone macrophages that support osteoblast differentiation and bone formation. Despite their importance in bone homeostasis, their function in human bone metabolism and osteoporosis remains poorly understood, largely due to the lack of a standardized isolation protocol. Here, we [...] Read more.
Osteal macrophages (“osteomacs”) are resident bone macrophages that support osteoblast differentiation and bone formation. Despite their importance in bone homeostasis, their function in human bone metabolism and osteoporosis remains poorly understood, largely due to the lack of a standardized isolation protocol. Here, we present a protocol for isolating primary human osteomacs from femoral head specimens obtained during arthroplasty. After the removal of bone marrow to minimize contamination with marrow-derived macrophages, bone fragments were enzymatically digested and osteomacs were isolated using CD14-based MACS® or CD14/CD45/ALP-based FACS. Immunofluorescence confirmed macrophage identity and revealed expression of markers associated with both M1-like and M2-like activation states. Isolated cells displayed heterogeneous morphology and could be maintained in culture. This protocol enables reproducible isolation of human osteomacs and provides a foundation for translational studies investigating osteoimmune interactions in bone disease and osteoporosis. Full article
(This article belongs to the Special Issue Recent Advances in Bone Biology)
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