Microorganisms Engineering and Gene-Editing Methods
A special issue of Life (ISSN 2075-1729). This special issue belongs to the section "Genetics and Genomics".
Deadline for manuscript submissions: 30 August 2025 | Viewed by 225
Special Issue Editors
Interests: metabolic engineering; genome editing; bioinformatics; enzyme engineering
Special Issues, Collections and Topics in MDPI journals
Interests: metabolic engineering; biobased materials and chemicals; gene expression regulation; bacterial stress response
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
Engineered microorganisms have emerged as critical assets across diverse sectors, including industrial biotechnology, biomedicine, agriculture, and environmental science. The precise construction and optimization of these strains necessitate advanced gene-editing techniques. Gene editing is not only fundamental to scientific progress but has also dramatically transformed our understanding of microorganisms. While significant progress has been achieved in recent decades, there remains considerable potential for refining these techniques to expand their applicability and address unmet challenges in various fields.
This Special Issue, titled "Microorganisms Engineering and Gene-Editing Methods", aims to present a comprehensive collection of articles exploring various facets of microorganism gene editing and its applications. The topics covered in this Issue include, but are not limited to the following:
- Novel gene-editing techniques for bacteria, archaea, fungi, and other microbial systems.
- Improvements in editing efficiency, specificity, and scalability, including strategies to minimize off-target effects and cytotoxicity.
- Advancements in workflow optimization, such as transformation protocols, recombination systems, counter-selection methods, recombinase engineering, and plasmid curing.
- Emerging CRISPR-based technologies, including CRISPR interference (CRISPRi), CRISPR activation (CRISPRa), base editors, prime editors, RNA-targeting systems, and CRISPR-associated transposase tools.
- Metabolic engineering, strain optimization for bioproduction, functional analysis of microbial pathways, and the enhancement of traits through gene editing. The precise expression of exogenous genes to endow microorganisms with new characteristics is also encouraged.
We encourage researchers from academia and industry to contribute their work, fostering interdisciplinary dialogue and accelerating the translation of microbial engineering into real-world solutions.
Dr. Jichao Wang
Prof. Dr. Guang Zhao
Dr. Xia Zhao
Guest Editors
Manuscript Submission Information
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Keywords
- microorganisms engineering
- metabolic engineering
- strain optimization
- gene editing
- CRISPR
- recombination
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