Non-Conventional Approaches in Modern Biological Analysis and Medical Diagnostics

A special issue of Life (ISSN 2075-1729).

Deadline for manuscript submissions: 31 July 2026 | Viewed by 284

Special Issue Editors


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Guest Editor
Department of Pharmaceutical Microbiology and Laboratory Diagnostics, National Medicines Institute, Warsaw, Poland
Interests: microbiology; enterobacterales; antibiotic resistance; β-lactamases; carbapenemases; biological sample testing
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Guest Editor Assistant
Department of Falsified Medicines and Medical Devices, National Medicines Institute, Chełmska 30/34 Str., 00-725 Warsaw, Poland
Interests: luminescence of organic compounds; vitamins; chemometric; statistical analysis

Special Issue Information

Dear Colleagues,

There is an increasing need for versatile yet sensitive labels, posed by demands for minute detection in biological samples. The topics of this Special Issue include, but are not limited to, the application of non-conventional analytical techniques such as infrared and EPR spectroscopy, luminescence, flow cytometry and thermogravimetric analysis. These assays can be used for the determination of molecules and enzymes’ biological activity (eg. vitamins, antioxidants, pharmacological active ingredients, ATP, β-lactamases) in analytical and microbiological diagnostics and protein immunoidentification, not only including the application of those in immunodiagnostics (eg. immunoglobulins). Making improvements in the rapid detection and identification of these substances has emerged as a global analytical challenge involving the large range of these molecules’ chemical structures. Researchers working on developing and validating methods, including separation and extraction methods, etc., are cordially invited to contribute an article to this Special Issue.

Prof. Dr. Anna Baraniak
Guest Editor

Dr. Paweł Rudnicki-Velasquez
Guest Editor Assistant

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Keywords

  • biological samples
  • non-conventional analytical techniques
  • life science
  • diagnostics
  • proteins

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Published Papers (1 paper)

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Review

35 pages, 1280 KB  
Review
Luminescence-Based Strategies for Detecting β-Lactamase Activity: A Review of the Last Decade
by Michał Jakub Korytkowski, Anna Baraniak, Alicja Boryło and Paweł Rudnicki-Velasquez
Life 2026, 16(2), 250; https://doi.org/10.3390/life16020250 - 2 Feb 2026
Abstract
Rapid detection of β-lactamase activity is becoming increasingly important as β-lactam resistance spreads at an alarming rate and conventional diagnostics often require several hours to deliver actionable results. Over the past decade, methods based on luminescence, bioluminescence, chemiluminescence, and fluorescence have become powerful [...] Read more.
Rapid detection of β-lactamase activity is becoming increasingly important as β-lactam resistance spreads at an alarming rate and conventional diagnostics often require several hours to deliver actionable results. Over the past decade, methods based on luminescence, bioluminescence, chemiluminescence, and fluorescence have become powerful tools for the functional assessment of resistance resulting from β-lactamase activity. These approaches provide highly sensitive, activity-based readouts, often within minutes, and frequently rely on simple optical instrumentation. In this review, we summarize recent developments in luminescent probe design between 2015 and 2025, with emphasis on reaction mechanisms, analytical performance, and the ability of these systems to discriminate between different β-lactamases, including narrow-spectrum enzymes, AmpC, ESBL, and carbapenemases. We also discuss their applications in bacterial cultures, clinical isolates, complex biological matrices and, in some cases, in vivo models. While luminescent assays are not yet positioned to replace standard susceptibility testing, they offer a practical and increasingly robust complement to culture-based and molecular methods. The emerging trends highlighted here, such as self-immobilizing fluorogenic probes, chemiluminescent relay systems, nanomaterial-based sensors and AI-assisted mobile platforms, suggest that luminescent β-lactamase detection could play a meaningful role in future rapid diagnostics and resistance surveillance. Full article
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