Advances in the Control of Plant Fungal Pathogens

A special issue of Journal of Fungi (ISSN 2309-608X). This special issue belongs to the section "Fungi in Agriculture and Biotechnology".

Deadline for manuscript submissions: closed (31 January 2026) | Viewed by 1943

Special Issue Editor


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Guest Editor
Food Biotechnology Department, Instituto de Agroquímica y Tecnología de Alimentos (IATA), Consejo Superior de Investigaciones Científicas (CSIC), Catedrático Agustín Escardino Benlloch 7, 46980 Paterna, Valencia, Spain
Interests: fungal biotechnology; synthetic biology; fungal control; fungicide resistance; fungal virulence; signal transduction pathways; host–pathogen interaction
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Special Issue Information

Dear Colleagues,

Fungi are responsible for a number of plant diseases that cause constant damage and losses affecting the productivity and quality of food, leading to spoilage and major economic losses. Plant pathogenic fungi can destroy up to 30% of agricultural products. Different treatments are used to prevent microbial spoilage, and among the most common are chemical fungicides. The strategies needed to control pathogenic fungi are evolving, largely due to the increasing incidence of fungicide  resistance and tightening regulations on the use of chemicals and market trends, which impose new challenges on food preservation.

This Special Issue aims to present and promote research on evolving alternatives in the control of plant pathogenic fungi.

Dr. Paloma Sánchez-Torres
Guest Editor

Manuscript Submission Information

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Keywords

  • plant diseases
  • fungicides
  • plant pathogenic fungi
  • control
  • biocontrol
  • antifungals

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Published Papers (2 papers)

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Research

22 pages, 3091 KB  
Article
Functional Characterization of BbroAFP Reveals Its Pleiotropic Antifungal Activity in Botrytis cinerea
by Arda Örçen, Yunus Doğan, Amjad Tulimat, Beyza Goncu, Batu Erman and Günseli Bayram Akçapınar
J. Fungi 2026, 12(5), 305; https://doi.org/10.3390/jof12050305 - 23 Apr 2026
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Abstract
Fungal pathogens pose a major threat to global agriculture and human health, necessitating alternative antifungal strategies with high efficacy and low resistance potential. Antifungal proteins (AFPs) from filamentous fungi are promising candidates due to their stability, selectivity, and diverse mechanisms of action. Here, [...] Read more.
Fungal pathogens pose a major threat to global agriculture and human health, necessitating alternative antifungal strategies with high efficacy and low resistance potential. Antifungal proteins (AFPs) from filamentous fungi are promising candidates due to their stability, selectivity, and diverse mechanisms of action. Here, we characterize Beauveria brongniartii antifungal protein (BbroAFP), a novel cysteine-rich protein from the entomopathogenic fungus B. brongniartii, and investigate its antifungal activity against Botrytis cinerea. Recombinant BbroAFP was expressed in Pichia pastoris, purified, and verified by liquid chromatography–tandem mass spectroscopy (LC–MS/MS) and in silico modeling. BbroAFP showed potent antifungal activity with minimum inhibitory concentrations (MICs) as low as 1 µM against several phytopathogenic fungi, while exhibiting no significant antibacterial activity. Activity was maintained across a wide range of pH and temperature conditions. Confocal microscopy revealed rapid surface binding followed by cytosolic internalization without major cell wall disruption. BbroAFP induced a rapid, transient burst of reactive oxygen species (ROS), accompanied by nuclear DNA fragmentation. Gene expression analysis revealed a transient increase in aif1, whereas mca1 expression decreased at later time points and mca2 remained largely unchanged, suggesting a metacaspase-independent response. Detached tomato leaf assays showed effective protection against B. cinerea without detectable phytotoxicity. Cytotoxicity assays confirmed a favorable safety profile, supporting further evaluation of BbroAFP for plant protection. Full article
(This article belongs to the Special Issue Advances in the Control of Plant Fungal Pathogens)
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16 pages, 1437 KB  
Article
Inhibitory Effect of Trichoderma longibrachiatum on Growth of Fusarium Species and Accumulation of Fumonisins
by Ruiqing Zhu, Ying Li, María Viñas, Qing Kong, Manlin Xu, Xia Zhang, Xinying Song, Kang He and Zhiqing Guo
J. Fungi 2026, 12(1), 49; https://doi.org/10.3390/jof12010049 - 10 Jan 2026
Viewed by 663
Abstract
Fusarium spp. cause devastating crop diseases and produce carcinogenic mycotoxins such as fumonisins, threatening global food safety and human health. In this study, Trichoderma longibrachiatum A25011, isolated from apples in Aksu, Xinjiang, exhibited significant antagonistic activity with mycelial growth inhibition rates of 54.52% [...] Read more.
Fusarium spp. cause devastating crop diseases and produce carcinogenic mycotoxins such as fumonisins, threatening global food safety and human health. In this study, Trichoderma longibrachiatum A25011, isolated from apples in Aksu, Xinjiang, exhibited significant antagonistic activity with mycelial growth inhibition rates of 54.52% against F. verticillioides 48.62% against F. proliferatum, and 58.22% against F. oxysporum in confrontation assays. Enzyme activity detection revealed high chitinase (583.21 U/mg protein) and moderate cellulase (43.92 U/mg protein) production, which may have the capacity to degrade fungal cell walls. High-Performance Liquid Chromatography–Mass Spectrometry (HPLC-MS/MS) analyses enabled the quantification of fungal hormones including gibberellin A3 (GA3, 2.44 mg/L), cytokinins (cis-zeatin riboside (CZR): 0.69 mg/L; trans-zeatin riboside (TZR): 0.004 mg/L; kinetin: 0.006 mg/L), and auxins (indole-3-acetic acid (IAA): 0.35 mg/L; abscisic acid: 0.06 mg/L). Application of a T. longibrachiatum A25011 spore suspension around the roots of peanut plants enhanced growth by 13.20% (height), 5.65% (stem and leaf biomass), and 39.13% (root biomass). Notably, A25011 reduced F. proliferatum-derived fumonisin accumulation in rice-based cultures by 93.58% (6 d) and 99.35% (10 d), suggesting biosynthetic suppression. The results demonstrated that T. longibrachiatum strain A25011 exhibited excellent biocontrol capability against Fusarium spp., proving its dual role in simultaneously suppressing fungal growth and fumonisin accumulation while promoting plant growth. T. longibrachiatum A25011 could be applied as a multifunctional biocontrol agent in sustainable agriculture in the future. Full article
(This article belongs to the Special Issue Advances in the Control of Plant Fungal Pathogens)
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