Novel Therapeutic Strategies in Wound Healing and Skin Regeneration

A special issue of Journal of Clinical Medicine (ISSN 2077-0383). This special issue belongs to the section "Emergency Medicine".

Deadline for manuscript submissions: closed (20 April 2021) | Viewed by 2641

Special Issue Editor


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Guest Editor
1. Herston Biofabrication Institute, Metro North Hospital and Health Service, Brisbane, QLD, Australia
2. Royal Brisbane and Women's Hospital, Metro North Hospital and Health Service, Brisbane, QLD, Australia
3. UQ Diamantina Institute, Translational Research Institute, The University of Queensland, Brisbane, QLD, Australia
Interests: regenerative medicine; wound care; biomaterials; 3D printing; tissue engineering; vascularization

Special Issue Information

Dear Colleagues,

Non-healing wounds and skin loss are major healthcare problems around the world costing healthcare systems billions of dollars a year. Recent technologies such as tissue engineering, biomaterial science, and in situ 3D printing have significantly advanced the development of skin tissue mimetics with clinical potential. The tissue-engineered skin models have also been used as tools to develop new therapeutics. Novel therapeutic strategies enabled the development of split or full-thickness skin substitutes to recapitulate the native functional skin.

Furthermore, numerous therapies such as immune cell, stem/progenitor cell therapies, as well as micro RNA- and siRNA-based techniques, and gene editing tools have been investigated to improve wound healing. Recent emerging techniques can reveal the mechanisms of skin regeneration and have immense potential to radically revolutionize the current situation. Future treatments may improve wound healing with reduced pain and contribute to a faster, more affordable, and scarless wound healing.

In this Special Issue, we will publish research on various emerging and state-of-the-art approaches being developed and implemented for accelerating wound healing and skin regeneration.

Dr. Abbas Shafiee
Guest Editor

Manuscript Submission Information

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Keywords

  • Wound healing
  • Cell therapy
  • micro RNA and siRNA
  • Tissue engineering and regenerative medicine
  • Scar treatments
  • Biomarker
  • Drug delivery

Published Papers (1 paper)

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Research

14 pages, 4595 KiB  
Article
Cytotoxicity and Epidermal Barrier Function Evaluation of Common Antiseptics for Clinical Use in an Artificial Autologous Skin Model
by María I. Quiñones-Vico, Ana Fernández-González, Elena Pérez-Castejón, Trinidad Montero-Vílchez and Salvador Arias-Santiago
J. Clin. Med. 2021, 10(4), 642; https://doi.org/10.3390/jcm10040642 - 8 Feb 2021
Cited by 10 | Viewed by 2257
Abstract
Bioengineered artificial skin substitutes (BASS) are the main treatment used in addition to autografts when skin injuries involve a large body surface area. Antiseptic/antibiotic treatment is necessary to prevent infections in the BASS implant area. This study aims to evaluate the effect of [...] Read more.
Bioengineered artificial skin substitutes (BASS) are the main treatment used in addition to autografts when skin injuries involve a large body surface area. Antiseptic/antibiotic treatment is necessary to prevent infections in the BASS implant area. This study aims to evaluate the effect of antiseptics and antibiotics on cell viability, structural integrity, and epidermal barrier function in BASS based on hyaluronic acid during a 28 day follow-up period. Keratinocytes (KTs) and dermal fibroblasts (DFs) were isolated from skin samples and used to establish BASS. The following antibiotic/antiseptic treatment was applied every 48 h: colistin (1%), chlorhexidine digluconate (1%), sodium chloride (0.02%), and polyhexanide (0.1%). Cell viability (LIVE/DEAD® assay), structural integrity (histological evaluation), and epidermal barrier function (trans-epidermal water loss, (TEWL), Tewameter®) were also evaluated. Cell viability percentage of BASS treated with chlorhexidine digluconate was significantly lower (p ≤ 0.001) than the other antiseptics at day 28. Compared to other treatments, chlorhexidine digluconate and polyhexanide significantly affected the epithelium. No significant differences were found regarding epidermal barrier. These results may be useful for treatment protocols after implantation of BASS in patients and evaluating them in clinical practice. BASS represent a suitable model to test in vitro the impact of different treatments of other skin wounds. Full article
(This article belongs to the Special Issue Novel Therapeutic Strategies in Wound Healing and Skin Regeneration)
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