Advances in Plant Cell and Organism Development
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Editors
Prof. Dr. Robert Hasterok
Prof. Dr. Robert Hasterok
E-Mail
Website
Collection Editor
Plant Cytogenetics and Molecular Biology Group, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 40-032 Katowice, Poland
Interests: plant molecular cytogenetics; structure and evolution of karyotypes; arrangement of chromosomes at interphase; nucleolar dominance; cytogenetics of meiosis; application of
Brachypodium as a model genus to study various aspects of plant nuclear genome structure; dynamics; (in)stability and evolution at the cytomolecular level
Special Issues, Collections and Topics in MDPI journals
Dr. Alexander Betekhtin
Dr. Alexander Betekhtin
E-Mail
Website
Collection Editor
Plant Cytogenetics and Molecular Biology Group, Institute of Biology, Biotechnology and Environmental Protection, Faculty of Natural Sciences, University of Silesia in Katowice, 40-032 Katowice, Poland
Interests: arabinogalactan proteins; cell cycle; cell wall; epigenetics; extensins; model plants; pectins; plant transformation; ploidy instability; proteomics; somatic embryogenesis; somaclonal variation; stem cells
Special Issues, Collections and Topics in MDPI journals
Topical Collection Information
Dear Colleagues,
After two successful editions (“Plant Cell and Organism Development” and “Plant Cell and Organism Development 2.0”) of our Special Issue, we decided to continue as a permanent Topic Collection.
Model organisms possess certain features that make them more amenable to scientific investigations than other, less tractable species. Today, many plant species are used as models in various studies, the most commonly used being Arabidopsis thaliana for dicots and rice and Brachypodium distachyon for monocots. They contribute significantly to our understanding of fundamental processes that govern various aspects of plant development in vivo and in vitro.
Thus, this Topic Collection addresses a wide range of topics linked with cell and plant development with a particular but not exclusive emphasis on the use of model plants. Recent research related, for example, to plant response to abiotic and biotic stresses; somatic embryogenesis; somaclonal variation; various cytological, cytogenetic, epigenetic, and genetic aspects of cell development; and other related topics are welcome.
Prof. Dr. Robert Hasterok
Dr. Alexander Betekhtin
Collection Editors
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the collection website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.
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Keywords
- Abiotic stress
- Biotic stress
- Cell cycle
- Chromosome number and integrity
- Cell wall
- Endoreplication
- Epigenetics
- Model plants
- Plant cell tissue culture
- Proteomics
- Somaclonal variation
- Somatic embryogenesis
Published Papers (6 papers)
Open AccessArticle
Arabidopsis thaliana MYC2 and MYC3 Are Involved in Ethylene-Regulated Hypocotyl Growth as Negative Regulators
by
Yuke Li, Ying Cheng, Fan Wei, Yingxiao Liu, Ruojia Zhu, Pingxia Zhao, Jing Zhang, Chengbin Xiang, Erfang Kang and Zhonglin Shang
Viewed by 481
Abstract
The ethylene-regulated hypocotyl elongation of
Arabidopsis thaliana involves many transcription factors. The specific role of MYC transcription factors in ethylene signal transduction is not completely understood. The results here revealed that two MYCs, MYC2 and MYC3, act as negative regulators in ethylene-suppressed hypocotyl
[...] Read more.
The ethylene-regulated hypocotyl elongation of
Arabidopsis thaliana involves many transcription factors. The specific role of MYC transcription factors in ethylene signal transduction is not completely understood. The results here revealed that two MYCs, MYC2 and MYC3, act as negative regulators in ethylene-suppressed hypocotyl elongation. Etiolated seedlings of the loss-of-function mutant of MYC2 or MYC3 were significantly longer than wild-type seedlings. Single- or double-null mutants of MYC2 and MYC3 displayed remarkably enhanced response to ACC(1-aminocyclopropane-1-carboxylate), the ethylene precursor, compared to wild-type seedlings. MYC2 and MYC3 directly bind to the promoter zone of ERF1, strongly suppressing its expression. Additionally, EIN3, a key component in ethylene signaling, interacts with MYC2 or MYC3 and significantly suppresses their binding to ERF1’s promoter. MYC2 and MYC3 play crucial roles in the ethylene-regulated expression of functional genes. The results revealed the novel role and functional mechanism of these transcription factors in ethylene signal transduction. The findings provide valuable information for deepening our understanding of their role in regulating plant growth and responding to stress.
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Open AccessArticle
Comparative Cytological and Gene Expression Analysis Reveals That a Common Wild Rice Inbred Line Showed Stronger Drought Tolerance Compared with the Cultivar Rice
by
Zijuan Huang, Peishan Huang, Shihui Chen, Mengzhu Hu, Hang Yu, Haibin Guo, Muhammad Qasim Shahid, Xiangdong Liu and Jinwen Wu
Viewed by 519
Abstract
Common wild rice (
Oryza rufipogon Griff.) is an important germplasm resource containing valuable genes. Our previous analysis reported a stable wild rice inbred line, Huaye3, which derives from the common wild rice of Guangdong Province. However, there was no information about its
[...] Read more.
Common wild rice (
Oryza rufipogon Griff.) is an important germplasm resource containing valuable genes. Our previous analysis reported a stable wild rice inbred line, Huaye3, which derives from the common wild rice of Guangdong Province. However, there was no information about its drought tolerance ability. Here, we assessed the germination characteristics and seedling growth between the Dawennuo and Huaye3 under five concentrations of PEG6000 treatment (0, 5%, 10%, 15%, and 20%). Huaye3 showed a stronger drought tolerance ability, and its seed germination rate still reached more than 52.50% compared with Dawennuo, which was only 25.83% under the 20% PEG6000 treatment. Cytological observations between the Dawennuo and Huaye3 indicated the root tip elongation zone and buds of Huaye3 were less affected by the PEG6000 treatment, resulting in a lower percentage of abnormalities of cortical cells, stele, and shrinkage of epidermal cells. Using the re-sequencing analysis, we detected 13,909 genes that existed in the genetic variation compared with Dawennuo. Of these genes, 39 were annotated as drought stress-related genes and their variance existed in the CDS region. Our study proved the strong drought stress tolerance ability of Huaye3, which provides the theoretical basis for the drought resistance germplasm selection in rice.
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Open AccessArticle
TOE1/TOE2 Interacting with GIS to Control Trichome Development in Arabidopsis
by
Yihua Liu, Shuaiqi Yang, Ali Raza Khan and Yinbo Gan
Cited by 6 | Viewed by 2045
Abstract
Trichomes are common appendages originating and projecting from the epidermal cell layer of most terrestrial plants. They act as a first line of defense and protect plants against different types of adverse environmental factors. GL3/EGL3-GL1-TTG1 transcriptional activator complex and GIS family genes regulate
[...] Read more.
Trichomes are common appendages originating and projecting from the epidermal cell layer of most terrestrial plants. They act as a first line of defense and protect plants against different types of adverse environmental factors. GL3/EGL3-GL1-TTG1 transcriptional activator complex and GIS family genes regulate trichome initiation through gibberellin (GA) signaling in
Arabidopsis. Here, our novel findings show that TOE1/TOE2, which are involved in developmental timing, control the initiation of the main-stem inflorescence trichome in
Arabidopsis. Phenotype analysis showed that the
35S:TOE1 transgenic line increases trichome density of the main-stem inflorescence in
Arabidopsis, while
35S:miR172b,
toe1,
toe2 and
toe1toe2 have the opposite phenotypes. Quantitative RT-PCR results showed that
TOE1/
TOE2 positively regulate the expression of
GL3 and
GL1. In addition, protein-protein interaction analysis experiments further demonstrated that TOE1/TOE2 interacting with GIS/GIS2/ZFP8 regulate trichome initiation in
Arabidopsis. Furthermore, phenotype and expression analysis also demonstrated that TOE1 is involved in GA signaling to control trichome initiation in
Arabidopsis. Taken together, our results suggest that TOE1/TOE2 interact with GIS to control trichome development in
Arabidopsis. This report could provide valuable information for further study of the interaction of TOE1/TOE2 with GIS in controlling trichome development in plants.
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Open AccessArticle
Roles of AGD2a in Plant Development and Microbial Interactions of Lotus japonicus
by
Mingchao Huang, Mengru Yuan, Chunyu Sun, Meiru Li, Pingzhi Wu, Huawu Jiang, Guojiang Wu and Yaping Chen
Viewed by 1973
Abstract
Arabidopsis AGD2 (Aberrant Growth and Death2) and its close homolog ALD1 (AGD2-like defense response protein 1) have divergent roles in plant defense. We previously reported that modulation of salicylic acid (SA) contents by
ALD1 affects numbers of nodules produced by
Lotus japonicus,
[...] Read more.
Arabidopsis AGD2 (Aberrant Growth and Death2) and its close homolog ALD1 (AGD2-like defense response protein 1) have divergent roles in plant defense. We previously reported that modulation of salicylic acid (SA) contents by
ALD1 affects numbers of nodules produced by
Lotus japonicus, but
AGD2′s role in leguminous plants remains unclear. A combination of enzymatic analysis and biological characterization of genetic materials was used to study the function of
AGD2 (
LjAGD2a and
LjAGD2b) in
L. japonicus. Both
LjAGD2a and
LjAGD2b could complement
dapD and
dapE mutants of
Escherichia coli and had aminotransferase activity in vitro.
ljagd2 plants, with insertional mutations of
LjAGD2, had delayed flowering times and reduced seed weights. In contrast, overexpression of
LjAGD2a in
L. japonicus induced early flowering, with increases in seed and flower sizes, but reductions in pollen fertility and seed setting rates. Additionally,
ljagd2a mutation resulted in increased expression of nodulin genes and corresponding increases in infection threads and nodule numbers following inoculation with
Rhizobium. Changes in expression of
LjAGD2a in
L. japonicus also affected endogenous SA contents and hence resistance to pathogens. Our results indicate that LjAGD2a functions as an LL-DAP aminotransferase and plays important roles in plant development. Moreover,
LjAGD2a activates defense signaling via the Lys synthesis pathway, thereby participating in legume–microbe interaction.
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Open AccessArticle
BIG Modulates Stem Cell Niche and Meristem Development via SCR/SHR Pathway in Arabidopsis Roots
by
Zhongming Liu, Ruo-Xi Zhang, Wen Duan, Baoping Xue, Xinyue Pan, Shuangchen Li, Peng Sun, Limin Pi and Yun-Kuan Liang
Cited by 4 | Viewed by 2488
Abstract
BIG, a regulator of polar auxin transport, is necessary to regulate the growth and development of Arabidopsis. Although mutations in the
BIG gene cause severe root developmental defects, the exact mechanism remains unclear. Here, we report that disruption of the
BIG gene resulted
[...] Read more.
BIG, a regulator of polar auxin transport, is necessary to regulate the growth and development of Arabidopsis. Although mutations in the
BIG gene cause severe root developmental defects, the exact mechanism remains unclear. Here, we report that disruption of the
BIG gene resulted in decreased quiescent center (QC) activity and columella cell numbers, which was accompanied by the downregulation of
WUSCHEL-RELATED HOMEOBOX5 (
WOX5) gene expression. BIG affected auxin distribution by regulating the expression of PIN-FORMED proteins (PINs), but the root morphological defects of
big mutants could not be rescued solely by increasing auxin transport. Although the loss of
BIG gene function resulted in decreased expression of the
PLT1 and
PLT2 genes, genetic interaction assays indicate that this is not the main reason for the root morphological defects of
big mutants. Furthermore, genetic interaction assays suggest that BIG affects the stem cell niche (SCN) activity through the SCRSCARECROW (SCR)/SHORT ROOT (SHR) pathway and BIG disruption reduces the expression of
SCR and
SHR genes. In conclusion, our findings reveal that the
BIG gene maintains root meristem activity and SCN integrity mainly through the SCR/SHR pathway.
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Open AccessReview
Buckwheat in Tissue Culture Research: Current Status and Future Perspectives
by
Alicja Tomasiak, Meiliang Zhou and Alexander Betekhtin
Cited by 13 | Viewed by 3337
Abstract
Buckwheat is a member of a genus of 23 species, where the two most common species are
Fagopyrum esculentum (common buckwheat) and
Fagopyrum tataricum (Tartary buckwheat). This pseudocereal is a source of micro and macro nutrients, such as gluten-free proteins and amino acids,
[...] Read more.
Buckwheat is a member of a genus of 23 species, where the two most common species are
Fagopyrum esculentum (common buckwheat) and
Fagopyrum tataricum (Tartary buckwheat). This pseudocereal is a source of micro and macro nutrients, such as gluten-free proteins and amino acids, fatty acids, bioactive compounds, dietary fibre, fagopyrins, vitamins and minerals. It is gaining increasing attention due to its health-promoting properties. Buckwheat is widely susceptible to in vitro conditions which are used to study plantlet regeneration, callus induction, organogenesis, somatic embryogenesis, and the synthesis of phenolic compounds. This review summarises the development of buckwheat in in vitro culture and describes protocols for the regeneration of plantlets from various explants and differing concentrations of plant growth regulators. It also describes callus induction protocols as well as the role of calli in plantlet regeneration. Protocols for establishing hairy root cultures with the use of
Agrobacterium rhizogens are useful in the synthesis of secondary metabolites, as well as protocols used for transgenic plants. The review also focuses on the future prospects of buckwheat in tissue culture and the challenges researchers are addressing.
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