Imaging with Fluorescent Proteins

Dear Colleagues,

Green fluorescent protein (GFP) opened an era of genetically-encoded fluorescent probes. The labeling of target proteins with GFP and homologous fluorescent proteins made it possible to directly visualize their localization, dynamics and interactions in live cells. Besides, genetically-encoded sensors shed light on diverse signalling events. Novel types of bipartite fluorescent tags based on combinations of protein hosts with endogenous or artificial fluorophores are the focus of recent developments. Still, to study complex biological phenomena in detail using different modalities—from whole-body imaging to nanoscopy—researchers need new fluorescent tags and sensors of various colors, fluorescence lifetimes and photoswitching behavior, possessing enhanced brightness and photostability at the ensemble and single-molecule levels.

This Special Issue aims to collect original and review papers on the development and applications of fluorescent protein-based tools. Works on multiparameter imaging and nanoscopy of live cells as well as the development of novel types of fluorescent proteins are especially welcome.

Prof. Dr. Konstantin A. Lukyanov
Guest Editor

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