Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology
1
Institut de Pharmacologie et de Biologie Structurale, IPBS, Université de Toulouse, CNRS, UPS, 31077 Toulouse, France
2
Centre de Recherche en Cancérologie de Toulouse (CRCT), Inserm, Université Paul Sabatier-Toulouse III, CNRS, 31037 Toulouse, France
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(14), 3479; https://doi.org/10.3390/ijms20143479
Received: 15 June 2019 / Revised: 8 July 2019 / Accepted: 8 July 2019 / Published: 15 July 2019
(This article belongs to the Special Issue Imaging with Fluorescent Proteins)
Molecular engineering of the green fluorescent protein (GFP) into a robust and stable variant named Superfolder GFP (sfGFP) has revolutionized the field of biosensor development and the use of fluorescent markers in diverse area of biology. sfGFP-based self-associating bipartite split-FP systems have been widely exploited to monitor soluble expression in vitro, localization, and trafficking of proteins in cellulo. A more recent class of split-FP variants, named « tripartite » split-FP, that rely on the self-assembly of three GFP fragments, is particularly well suited for the detection of protein–protein interactions. In this review, we describe the different steps and evolutions that have led to the diversification of superfolder and split-FP reporter systems, and we report an update of their applications in various areas of biology, from structural biology to cell biology.
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Keywords:
fluorescent protein; superfolder; split-GFP; bipartite; tripartite; folding; PPI
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
MDPI and ACS Style
Pedelacq, J.-D.; Cabantous, S. Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology. Int. J. Mol. Sci. 2019, 20, 3479. https://doi.org/10.3390/ijms20143479
AMA Style
Pedelacq J-D, Cabantous S. Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology. International Journal of Molecular Sciences. 2019; 20(14):3479. https://doi.org/10.3390/ijms20143479
Chicago/Turabian StylePedelacq, Jean-Denis; Cabantous, Stéphanie. 2019. "Development and Applications of Superfolder and Split Fluorescent Protein Detection Systems in Biology" Int. J. Mol. Sci. 20, no. 14: 3479. https://doi.org/10.3390/ijms20143479
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