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Fluorescent Protein-Based Indicators for Functional Super-Resolution Imaging of Biomolecular Activities in Living Cells
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Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging

1
Sorbonne Université, École Normale Supérieure, PSL University, CNRS, Laboratoire des Biomolécules, LBM, 75005 Paris, France
2
Institut Universitaire de France (IUF), 1 rue Descartes, 75005 Paris, France
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(24), 6142; https://doi.org/10.3390/ijms20246142
Received: 22 November 2019 / Revised: 4 December 2019 / Accepted: 4 December 2019 / Published: 5 December 2019
(This article belongs to the Special Issue Imaging with Fluorescent Proteins)
Our ability to observe biochemical events with high spatial and temporal resolution is essential for understanding the functioning of living systems. Intrinsically fluorescent proteins such as the green fluorescent protein (GFP) have revolutionized the way biologists study cells and organisms. The fluorescence toolbox has been recently extended with new fluorescent reporters composed of a genetically encoded tag that binds endogenously present or exogenously applied fluorogenic chromophores (so-called fluorogens) and activates their fluorescence. This review presents the toolbox of fluorogen-based reporters and biosensors available to biologists. Various applications are detailed to illustrate the possible uses and opportunities offered by this new generation of fluorescent probes and sensors for advanced bioimaging. View Full-Text
Keywords: fluorogenic systems; protein labeling; RNA labeling; advanced microscopy fluorogenic systems; protein labeling; RNA labeling; advanced microscopy
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Péresse, T.; Gautier, A. Next-Generation Fluorogen-Based Reporters and Biosensors for Advanced Bioimaging. Int. J. Mol. Sci. 2019, 20, 6142.

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