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Open AccessArticle

Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins

1
Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia
2
Pirogov Russian National Research Medical University, Ostrovitianov 1, 117997 Moscow, Russia
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2018, 19(12), 3778; https://doi.org/10.3390/ijms19123778
Received: 8 November 2018 / Revised: 23 November 2018 / Accepted: 24 November 2018 / Published: 28 November 2018
(This article belongs to the Special Issue Imaging with Fluorescent Proteins)
Fluorogens are an attractive type of dye for imaging applications, eliminating time-consuming washout steps from staining protocols. With just a handful of reported fluorogen-protein pairs, mostly in the green region of spectra, there is a need for the expansion of their spectral range. Still, the origins of solvatochromic and fluorogenic properties of the chromophores suitable for live-cell imaging are poorly understood. Here we report on the synthesis and labeling applications of novel red-shifted fluorogenic cell-permeable green fluorescent protein (GFP) chromophore analogs. View Full-Text
Keywords: fluorogenic dyes; arylmethane dyes; GFP chromophore; fluorescent labeling; fluorescence microscopy; protein-PAINT fluorogenic dyes; arylmethane dyes; GFP chromophore; fluorescent labeling; fluorescence microscopy; protein-PAINT
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MDPI and ACS Style

Bozhanova, N.G.; Baranov, M.S.; Baleeva, N.S.; Gavrikov, A.S.; Mishin, A.S. Red-Shifted Aminated Derivatives of GFP Chromophore for Live-Cell Protein Labeling with Lipocalins. Int. J. Mol. Sci. 2018, 19, 3778.

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