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Molecular Insights into Antimicrobial Agents: Mechanisms and Applications

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: 20 May 2026 | Viewed by 1058

Special Issue Editor


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Special Issue Information

Dear Colleagues,

We can announce an upcoming Special Issue entitled “Molecular Insights into Antimicrobial Agents: Mechanisms and Applications”. In an era of increasing bacterial resistance to the most available antibiotics, it is necessary to search for new substances with antibacterial activities. In addition to finding novel compounds effective regardless bacterial acquired resistance, it is vital to understand the mechanisms of action of these substances’ influence on microbial cells.

In this Special Issue, we encourage scholars in the field to submit scientific papers, including research articles, scientific communications, and review articles, dealing both with the discovery of new antibacterial molecules and their possible applications and scope of action, as well as papers explaining their possible mechanisms. Publications should focus on molecular aspects, but this Special Issue is not limited to that topic. We also request articles on current solutions or new strategies for combating resistant pathogens based on the nature of their developed resistance. 

Submissions should cover topic such as the following:

  • The development of novel antimicrobial agents accompanied by the comprehensive elucidation of their modes of action;
  • The in-depth investigation of resistance mechanisms and formulation of strategic interventions to mitigate them;
  • Cutting-edge methods for assessing the effectiveness of antimicrobial treatments both qualitatively and quantitatively;
  • Combining known and innovative antibacterial compounds and other strategies for the more effective inhibition of pathogens and biofilms;
  • Interdisciplinary research combining microbiology with emerging fields such as nanotechnology, synthetic biology, bioengineering, etc., as well as sciences devoted to designing possible new antimicrobials and predicting their efficacy;
  • The applications and modes of use of antibacterial substances in medicine, agriculture, industry, and aquaculture. 

Dr. Aleksandra Maria Kocot
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • antimicrobial compounds
  • antibacterial activity
  • antibiotic resistance
  • pathogen-tailored therapeutic targeting
  • novel antimicrobials
  • molecular aspects of the mechanisms of action of antibacterial substances
  • effectiveness of antibacterial substances

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Published Papers (2 papers)

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Research

21 pages, 5766 KB  
Article
Activity and Biocompatibility Evaluation of Enzybiotic Compositions Formulated with Azotobacter vinelandii Alginate for Topical Use
by Arina A. Klimova, Ekaterina A. Trusova, Elizaveta A. Akoulina, Nataliia P. Antonova, Andrei A. Dudun, Michail Yu. Orlov, Marina Yu. Kochevalina, Vera V. Voinova, Anton P. Bonartsev and Daria V. Vasina
Int. J. Mol. Sci. 2026, 27(9), 3856; https://doi.org/10.3390/ijms27093856 - 27 Apr 2026
Abstract
Due to their availability and environmental friendliness, alginate polymers are widely used in pharmaceuticals and cosmetics. The most common type of alginate is derived from seaweed and is used to develop topical dosage forms, among other things. However, variability in the seaweed material [...] Read more.
Due to their availability and environmental friendliness, alginate polymers are widely used in pharmaceuticals and cosmetics. The most common type of alginate is derived from seaweed and is used to develop topical dosage forms, among other things. However, variability in the seaweed material can lead to instability in the physicochemical parameters. Biotechnologically produced alginate minimizes this drawback through controlled synthesis. However, unlike algal alginates, the safety profile of such polymers has not been well studied. When developing dosage forms intended for wound surfaces, safety is of primary importance. In this study, we developed enzybiotic compositions based on bacterial alginate as an excipient and a novel recombinant modified endolysin, LysSi3-LK, as an antibacterial agent, and assessed their antibacterial properties and safety profile. The study included an in vitro evaluation of the activity spectrum, as well as the cytotoxicity and biocompatibility, of gel and hydrogel compositions. It was demonstrated that bacterial alginate is acceptable for the encapsulation of endolysin. It exhibited medium cytotoxic effects on the HaCaT cells, which were significantly reduced by the LysSi3-LK addition. The migration of cells was diminished following exposure to the gel and hydrogel formulations. However, an improvement in biocompatibility was observed in the cell proliferation assay. Full article
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15 pages, 981 KB  
Article
CGL, a Lectin from Crenomytilus grayanus, Exhibits Antibiofilm and Synergistic Antibacterial Activity Against Escherichia coli and Staphylococcus aureus
by Irina V. Chikalovets, Tatyana O. Mizgina, Olga I. Nedashkovskaya, Linhe Su, Kuo-Feng Hua, Xiangqian Jia, Yanlong Zhang and Oleg V. Chernikov
Int. J. Mol. Sci. 2026, 27(4), 1961; https://doi.org/10.3390/ijms27041961 - 18 Feb 2026
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Abstract
Lectins are carbohydrate-binding proteins that specifically bind to sugar groups associated with other molecules. Several studies have reported that these proteins can also modulate the activity of antibiotics against multidrug-resistant (MDR) strains in addition to interacting with carbohydrates. This study reports that gentamicin [...] Read more.
Lectins are carbohydrate-binding proteins that specifically bind to sugar groups associated with other molecules. Several studies have reported that these proteins can also modulate the activity of antibiotics against multidrug-resistant (MDR) strains in addition to interacting with carbohydrates. This study reports that gentamicin exhibits enhanced antibacterial activity against Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive) bacterial strains when complexed with Crenomytilus grayanus lectin (CGL). Enzyme-linked lectin, thermofluor, and isothermal titration calorimetry assays revealed that gentamicin interacts with CGL through a domain distinct from the carbohydrate recognition domain. An increase in antibacterial activity was observed when lectin and antibiotic were used together against S. aureus in living systems—specifically, sea urchin (Strongylocentrotus nudus) embryos. Full article
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