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Molecular Research and Utilization of Environmental Microbial Resources

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 20 September 2026 | Viewed by 1263

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Guest Editor
Department of Health Sciences, University of Jaén, Jaén, Spain
Interests: probiotics; prebiotics; intestinal health; microbiology; antimicrobials; microbial molecular biology; antibiotic resistance; food microbiology; lactic acid bacteria; bacteriocins
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Special Issue Information

Dear Colleagues,

Microorganisms represent one of the most abundant and versatile resources in the natural environment, playing essential roles in ecosystem functioning, nutrient cycling and biochemical processes. Environmental microbial resources, encompassing bacteria, archaea, fungi and microalgae from diverse habitats such as soil, water, plants and extreme environments, hold enormous potential for biotechnological innovation and sustainable applications. Their metabolic diversity enables their use in agriculture, food production, bioenergy, bioremediation and the development of novel bio-based products.

Recent advances in cultivation techniques, high-throughput sequencing, omics technologies and systems biology have greatly expanded our ability to discover, characterize and utilize environmental microbial communities. Beyond community-level insights, molecular research has revealed key microbial resources such as genes, enzymes, metabolites and biosynthetic gene clusters that drive these applications. Examples include nitrogen fixation genes in biofertilizer development, lytic enzymes and antimicrobial peptides for biocontrol, hydrolytic enzymes and lignocellulolytic pathways for bioenergy, as well as novel secondary metabolites with pharmaceutical or agricultural potential. Such findings provide the molecular blueprint for exploiting microbial biodiversity and accelerating translation into practice.

This Special Issue, Molecular Research and Utilization of Environmental Microbial Resources”, invites original research articles, reviews and perspectives covering all aspects of environmental microbiology on a molecular level related to microbial diversity, functional potential and sustainable applications. Contributions may address microbial resource discovery, functional characterization, ecological roles, molecular mechanisms, biotechnological exploitation and the development of innovative tools to explore and utilize environmental microbiomes.

Prof. Dr. Hikmate Abriouel
Guest Editor

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Keywords

  • environmental microbiology
  • molecular microbial resources
  • microbial metabolites
  • microbial diversity
  • microbial ecology
  • bioremediation
  • agricultural microbiology
  • omics technologies
  • biofilm formation
  • microbiome applications

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Published Papers (2 papers)

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Research

19 pages, 3985 KB  
Article
Biotransformation of Aliphatic and Aromatic Hydrocarbons by Aerobic Bacterial Strains Isolated from Brown Coal
by Paulina Supel, Katarzyna Starzec, Piotr Kapusta, Joanna Brzeszcz and Paweł Kaszycki
Int. J. Mol. Sci. 2026, 27(3), 1407; https://doi.org/10.3390/ijms27031407 - 30 Jan 2026
Viewed by 272
Abstract
Lignite collected from a brown coal deposit was colonized with fully aerobic bacteria exhibiting hydrocarbon biodegradation pathways. Six autochthonous strains were isolated and tested for tolerance and biotransformation potential towards various xenobiotics such as hexadecane, squalane, pristane, benzoic acid, naphthalene, phenanthrene, and diesel [...] Read more.
Lignite collected from a brown coal deposit was colonized with fully aerobic bacteria exhibiting hydrocarbon biodegradation pathways. Six autochthonous strains were isolated and tested for tolerance and biotransformation potential towards various xenobiotics such as hexadecane, squalane, pristane, benzoic acid, naphthalene, phenanthrene, and diesel oil. After preliminary screening, four xenobiotic-resistant strains were selected (Rhodococcus opacus CUP11, Pseudomonas fluorescens CUP15, Sphingobacterium sp. CUP16, and Rhodococcus sp. CUP17) and further treated for 14 days under aerobic conditions with variant concentrations of each compound (1, 2.5, 5 and 10 g/dm3). Microbial population dynamics and xenobiotic level changes were monitored. Rhodococcus opacus CUP11 and Rhodococcus sp. CUP17 were the most metabolically versatile bacteria capable of biotransforming several xenobiotics. Among the best-performing strains, the highest degradation yields were obtained for CUP17 (81% removal of diesel oil applied at 10 g/dm3, 99% of 2.5 g/dm3 hexadecane and 27% of 1 g/dm3 squalane), and CUP11 (49% of 10 g/dm3 hexadecane and 48% of 1 g/dm3 pristane). The strain CUP16 utilized squalane (33% at 1 g/dm3). The results suggest that the lignite-indigenous bacteria may be applicable for bioremediation of persistent xenobiotics in environmental cleanup projects. Full article
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25 pages, 2567 KB  
Article
Unravelling the Molecular Responses of the Yeast Schwanniomyces etchellsii to Hyperosmotic Stress in Seawater Medium Using Omic Approaches
by Cecilia Andreu, Èlia Obis and Marcel·lí del Olmo
Int. J. Mol. Sci. 2026, 27(1), 183; https://doi.org/10.3390/ijms27010183 - 23 Dec 2025
Viewed by 660
Abstract
Schwanniomyces etchellsii is an unconventional, halotolerant microorganism. Like some other yeasts, it can efficiently perform various biocatalytic transformations of organic compounds in seawater more effectively than in freshwater. In seawater, conversion rates are higher, by-product production is minimized, greater substrate loading is possible, [...] Read more.
Schwanniomyces etchellsii is an unconventional, halotolerant microorganism. Like some other yeasts, it can efficiently perform various biocatalytic transformations of organic compounds in seawater more effectively than in freshwater. In seawater, conversion rates are higher, by-product production is minimized, greater substrate loading is possible, and cells can be recycled for further use. To identify the molecular features that explain this behavior, comparative proteomic and lipidomic studies were conducted on cells grown in seawater and freshwater at various growth stages. The results showed higher expression of proteins involved in the stress response, such as glycerol-3-phosphate dehydrogenase, the glycerol transporter Stl1 and the P-type ATPase sodium pump Ena1, and several phospholipid biosynthesis proteins, including inositol-3-phosphate synthase and phosphatidate cytidylyltransferase, in seawater. Changes in metabolic enzymes and other proteins involved in responding to stimuli were also observed between the two conditions. Overall, cells grown in a freshwater medium exhibited higher levels of enzymes involved in biosynthetic processes. Differences in lipid profiles were also observed between cells grown in the two media. Higher levels of monoacyl and diacylglycerols were found in seawater, while higher levels of phospholipids containing serine and ethanolamine were found in freshwater. Consistent with more permeable membranes, cells grown in seawater exhibited lower levels of ergosterol. Full article
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